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Mkl1 (NM_153049) Mouse Tagged ORF Clone
CAT＃: MG227406
- TrueORF®
Mkl1 (tGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_153049" in other vectors (3)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AMKL; Bsac; Mal; Mrtf-A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227406 representing NM_153049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTGCTGGAGCCTGAGATGCTAATGATGGCTGTGCAGTCAGTGCTGCAGTTGAAGCTCCAGCAGC GGCGGACCCGGGAGGAACTGGTGAGCCAAGGGATCATGCCGCCTTTGAAAAGCCCCGCTGCATTTCATGA GCAGAGAAGAAGCCTGGAGCGGGCCAGGACCGAGGACTATTTGAAACGGAAGATCCGTTCCCGGCCCGAG AGAGCAGAGCTGGTCAGGATGCACATTCTGGAAGAGACCTCGGCTGAGCCTTCGCTCCAGGCCAAGCAGC TGAAGCTGAAGAGAGCCAGGCTGGCTGATGACCTCAATGAAAAGATTGCACAGAGGCCTGGCCCCATGGA GCTGGTGGAGAAGAATATCCTGCCTGTGGAGTCCAGCCTGAAGGAGGCTATCATTGTGGGCCAGGTAAAT TACCCAAAGGTAGCAGACAGTTCCTCCTTCGACGAGGACAGCAGCGATGCCCTGTCTCCTGAGCAGCCTG CCAGCCATGAGTCCCAGGGTTCAGTGCCATCACCCTTGGAGTCCCGAGTCAGTGATCCACTGCCCAGTGC CACCTCCATATCACCCACTCAGGTTCTTTCTCAACTCCCAATGGCTCCGGATCCTGGAGAGACGCTTTTT CTGGCAGAGCAGCCTCCTCTGCCTCCCGCACCTCTGCTGCCCCCAAGCCTAGCCAATGGAAGCATCGTCC CCACTGCCAAGCCTGCTCCCACACTCATCAAGCAAAGCCAACCCAAGTCTGCCAGCGAGAAATCACAGCG CAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGAAGAAGCTCAAGTACCACCAGTACATCCCCCCGGAC CAGAAGCAGGACAAGGGGGCGCCCGCCATGGACTCCTCCTATGCCAAGATCCTGCAGCAGCAGCAGCTCT TCCTGCAGCTGCAGATCCTCAACCAGCAGCAGCAGCAGCAGCAGCAACAGCACTACAACTACCAGGCCAT CCTGCCTGCCCCTCCCAAGCCCTCGGCTGAGACTCCTGGAAGCAGTGCCCCTACCCCATCACGCAGCCTC TCCACCAGTAGCAGCCCCAGCTCAGGCACCCCAGGGCCCAGCGGGCTGGCACGCCAGAGCAGCACCGCAC TAGCTGCCAAACCAGGAGCCCTGCCAGCCAACCTGGATGACATGAAGGTGGCAGAGCTGAAGCAAGAACT GAAGTTGCGGTCCCTTCCCGTCTCAGGCACCAAGACAGAGCTGATAGAACGCCTGCGTGCCTACCAAGAC CAAGTCAGCCCAGCTCCAGGAGCCCCCAAGGCCCCTGCCACCACCTCTGTGCTGTCCAAGGCTGGTGAGG TAGTGGTCGCCTTCCCTGCGGCCCTGCTAAGCACAGGGTCAGCTCTTGTAACAGCAGGCCTTGCACCAGC TGAGATGGTGGTGGCCACAGTAACCAGCAATGGCATGGTGAAGTTTGGCAGCACAGGCTCCACACCCCCC GTGTCTCCCACCCCTTCAGAGCGCTCACTGCTCAGCACGGGTGATGAGAATTCCACACCTGGGGATGCCT TTGGTGAAATGGTGACATCGCCGCTGACACAGCTCACCCTGCAGGCCTCCCCACTGCAGATCGTGAAGGA GGAGGGTGCCCGTGCTGCGTCCTGCTGTCTAAGCCCTGGTGCTCGGGCTGAGCTGGAGGGACTGGACAAG GACCAGATGCTGCAGGAGAAGGACAAGCAGATTGAGGAGCTGACCCGAATGCTCCAACAGAAGCAGCAGC TGGTTGAGCTGCTGCGGCTACAGCTGGAGCAGCAGAAGCGGGCCCAGCAGCCAGCCCCAGCCAGCAGCCC TGTGAAGAGGGAAAGTGGTTTCTCCAGTTGCCAGCTGAGCTGCCAGCCCCAGGGCTCTGCCCATGCTTTT GGCTCTGGCCTAGTGGTTCCCACTACCAACCATGGAGACACTCAGGCCCCAGCGCCAGAGTCCCCACCTG TGGTGGTGAAGCAGGAAGCTGGGCCACCTGAGCCAGATCTGGCCCCCAGCTCCCAGCTGCTCTTGGGCTC CCAGGGCACCAGCTTCCTCAAGAGGGTCAGCCCTCCTACCCTGGTCACTGACTCTACAGGGACTCACCTC ATCCTCACTGTGACCAATAAGAGTGCTGATGGCCCTGGCTTGCCTGCAGGGAGCCCCCAGCAGCCCTTGT CCCAGCCTGGTTCTCCAGCCCCTGGTCCACCTGCCCAGATGGACCTGGAGCACCCACCTCAGCCTCCGTT TGCAACCCCCACATCTCTGCTGAAGAAGGAGCCCCCTGGTTATGAAGAGACTGTGACCCAGCAGCCTAAG CAGCAGGAAAATGGCTCCTCCAGTCAGCACATGGATGATCTGTTTGATATTCTTATTCAGAGTGGAGAGA TTTCAGCAGATTTCAAAGAGCCACCATCCCTACCAGGCAAGGAAAAGTCACCTCCAGCAGCAGCAGCGTA TGGGCCTCCATTGACACCACAACCCTCGCCTTTGAGTGAACTCCCCCAAGCTGCTCCTCCACCAGGTTCC CCCACCCTCCCAGGGCGCCTTGAAGACTTCCTGGAGAGCAGCACAGGGCTGCCCCTGCTGACAAGTGGGC ACGAGGGACCAGAACCCCTTTCCCTCATTGATGACCTCCACAGCCAGATGCTGAGCAGCTCCGCCATCCT GGACCACCCCCCATCACCCATGGACACCTCTGAATTGCACTTTGCTCCTGAGCCCAGCAGTGGTATGGGC CTGGACCTGGCTGTTGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCTGGTGGCCCTGTGCTCA GCCTGGCTCCCCTCAGCACTGCAGCCCCCAGCCTCTTCTCGATGGACTTCCTGGATGGACACGACTTGCA GCTCCACTGGGATTCCTGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227406 representing NM_153049
Red=Cloning site Green=Tags(s) MTLLEPEMLMMAVQSVLQLKLQQRRTREELVSQGIMPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPE RAELVRMHILEETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVN YPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLESRVSDPLPSATSISPTQVLSQLPMAPDPGETLF LAEQPPLPPAPLLPPSLANGSIVPTAKPAPTLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPD QKQDKGAPAMDSSYAKILQQQQLFLQLQILNQQQQQQQQQHYNYQAILPAPPKPSAETPGSSAPTPSRSL STSSSPSSGTPGPSGLARQSSTALAAKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQD QVSPAPGAPKAPATTSVLSKAGEVVVAFPAALLSTGSALVTAGLAPAEMVVATVTSNGMVKFGSTGSTPP VSPTPSERSLLSTGDENSTPGDAFGEMVTSPLTQLTLQASPLQIVKEEGARAASCCLSPGARAELEGLDK DQMLQEKDKQIEELTRMLQQKQQLVELLRLQLEQQKRAQQPAPASSPVKRESGFSSCQLSCQPQGSAHAF GSGLVVPTTNHGDTQAPAPESPPVVVKQEAGPPEPDLAPSSQLLLGSQGTSFLKRVSPPTLVTDSTGTHL ILTVTNKSADGPGLPAGSPQQPLSQPGSPAPGPPAQMDLEHPPQPPFATPTSLLKKEPPGYEETVTQQPK QQENGSSSQHMDDLFDILIQSGEISADFKEPPSLPGKEKSPPAAAAYGPPLTPQPSPLSELPQAAPPPGS PTLPGRLEDFLESSTGLPLLTSGHEGPEPLSLIDDLHSQMLSSSAILDHPPSPMDTSELHFAPEPSSGMG LDLAVGHLDSMDWLELSSGGPVLSLAPLSTAAPSLFSMDFLDGHDLQLHWDSCL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153049 |
| ORF Size | 2892 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153049.3, NP_694629.2 |
| RefSeq Size | 4111 bp |
| RefSeq ORF | 2895 bp |
| Locus ID | 223701 |
| UniProt ID | Q8K4J6 |
| Gene Summary | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR227406 | Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 1 |
CNY 7,064.00 |
|
| MR227406L3 | Lenti ORF clone of Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 1 |
CNY 9,030.00 |
|
| MR227406L4 | Lenti ORF clone of Mkl1 (mGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 1 |
CNY 9,030.00 |
