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Nefm (NM_008691) Mouse Tagged ORF Clone
CAT＃: MG227367
- TrueORF®
Nefm (tGFP-tagged) - Mouse neurofilament medium polypeptide (Nefm), (10ug)
ORF Plasmid: DDK
"NM_008691" in other vectors (4)
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CNY 7,808.00
CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nef3; NF-M; NF160; NF165; Nfm |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227367 representing NM_008691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCT TCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCG GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAAC TGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGT GCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCG CACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACC ACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAG TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGA GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAA AGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGAC CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA ACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT CGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCAC AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGG AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGC CGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCT CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGA AATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCC GAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG AGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTC AGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAA GAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGG GAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTAT GCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAG GAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAA AGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGA GGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAG CCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCC CGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGA AACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCT GCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCA CCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGA GCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATA GTCAAGGAAGTCACCCAGGGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227367 representing NM_008691
Red=Cloning site Green=Tags(s) MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSS AESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQAS HAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEE SSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSD QNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERH NHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGP LYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEETLTAIAEELAASAKEEKEEA EEKEEEPEAEKSPVKSPEAKEEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEE EEGETEAEGEGEEAEAKEEKKIEGKVEEVAVKEEIKVEKPEKAKSPMPKSPVEEVKPKPEAKAGKGEHKE EEKVEEEKKEVTKESPKEEKVEKKEEKPKDVADKKKAESPVKEKAVEEVITISKSVKVSLEKDTKEEKLQ PQEKVKEKAEEEGGSEEEGSDRSPQESKKEDIAINGEVEGKEEEEQETQEKGSGREEEKGVVTNGLDVSP AEEKKGEDSSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAI VKEVTQGD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008691 |
| ORF Size | 2544 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008691.2, NP_032717.2 |
| RefSeq Size | 3244 bp |
| RefSeq ORF | 2547 bp |
| Locus ID | 18040 |
| UniProt ID | P08553 |
| Gene Summary | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222041 | Nefm (untagged) - Mouse neurofilament, medium polypeptide (Nefm), (10ug) |
CNY 10,830.00 |
|
| MR227367 | Nefm (Myc-DDK-tagged) - Mouse neurofilament, medium polypeptide (Nefm) |
CNY 6,208.00 |
|
| MR227367L3 | Lenti ORF clone of Nefm (Myc-DDK-tagged) - Mouse neurofilament, medium polypeptide (Nefm) |
CNY 9,310.00 |
|
| MR227367L4 | Lenti ORF clone of Nefm (mGFP-tagged) - Mouse neurofilament, medium polypeptide (Nefm) |
CNY 9,310.00 |
