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Ptk6 (NM_009184) Mouse Tagged ORF Clone
CAT＃: MG227251
- TrueORF®
Ptk6 (tGFP-tagged) - Mouse PTK6 protein tyrosine kinase 6 (Ptk6), (10ug)
ORF Plasmid: DDK
"NM_009184" in other vectors (4)
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CNY 5,256.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BRK; Sik; tks; Tksk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227251 representing NM_009184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTTGGGACAAGGCTCACCTGGGTCCTAAGTATGTGGGCCTCTGGGACTTCAAGGCACGGACAG ATGAGGAGCTGAGCTTTCAGGCAGGAGACCTCCTCCATGTTACCAAGAAGGAGGAACTGTGGTGGTGGGC CACCCTGCTGGATGCAGAAGGCAAGGCCTTGGCTGAGGGCTATGTGCCTCACAACTACCTGGCTGAGAAG GAAACTGTGGAGTCTGAACCGTGGTTCTTTGGTTGCATCTCCCGCTCAGAGGCCATGCACAGGCTGCAGG CTGAGGACAACTCGAAGGGTGCCTTCCTGATCAGAGTCAGCCAGAAGCCAGGAGCAGACTATGTCCTCTC TGTCCGGGATGCTCAGGCCGTGCGACATTACAGGATCTGGAAGAACAACGAGGGCCGGCTGCACCTGAAT GAGGCGGTATCCTTCTCCAATCTGTCTGAGCTTGTGGACTACCATAAGACCCAGAGCCTGTCTCATGGCC TACAGCTGTCCATGCCCTGCTGGAAGCACAAAACTGAGCCCTTGCCCCACTGGGATGACTGGGAGAGGCC GAGGGAGGAGTTCACACTCTGTAAGAAGCTGGGGGCCGGCTACTTTGGGGAGGTCTTTGAAGCGCTCTGG AAAGGCCAGGTCCATGTGGCTGTGAAGGTGATCTCTAGAGACAATCTCCTGCACCAGCACACCTTCCAGG CTGAGATTCAGGCCATGAAGAAGCTGCGGCACAAGCACATCCTGTCACTGTACGCTGTGGCGACTGCAGG GGACCCGGTCTACATCATCACGGAGCTCATGCCCAAGGGGAACCTGCTGCAGCTACTGCGTGACTCTGAT GAGAAAGCCCTGCCTATTTTGGAGCTGGTGGACTTTGCATCACAGGTTGCTGAGGGCATGTGCTACCTGG AATCTCAGAATTACATCCACCGTGACCTGGCTGCAAGGAACGTTCTTGTTACAGAGAACAATCTCTGCAA AGTGGGGGACTTTGGGCTTGCCAGGCTTGTCAAGGAGGACATCTACCTTTCCCATGAGCACAATGTCCCC TACAAATGGACAGCACCTGAGGCACTTTCCCGAGGGCATTACTCCATCAAGTCTGATGTCTGGTCTTTTG GAGTTCTTCTTCATGAAATTTTCAGCAGGGGGCAGATGCCCTACCCAGGCATGTCCAATCATGAAACCTT CCTGAGGGTGGATGCCGGCTACCGCATGCCCTGCCCTCTGGAGTGCCCACCCAACATACACAAGCTGATG CTCAGCTGCTGGAGCAGAGACCCCAAGCAGAGACCTTGCTTCAAAGACCTGTGTGAGAAACTCACAGGTA TCACCAGGTATGAGAACCTGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227251 representing NM_009184
Red=Cloning site Green=Tags(s) MVSWDKAHLGPKYVGLWDFKARTDEELSFQAGDLLHVTKKEELWWWATLLDAEGKALAEGYVPHNYLAEK ETVESEPWFFGCISRSEAMHRLQAEDNSKGAFLIRVSQKPGADYVLSVRDAQAVRHYRIWKNNEGRLHLN EAVSFSNLSELVDYHKTQSLSHGLQLSMPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALW KGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSD EKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHEHNVP YKWTAPEALSRGHYSIKSDVWSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLM LSCWSRDPKQRPCFKDLCEKLTGITRYENLV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009184 |
| ORF Size | 1353 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009184.2, NP_033210.1 |
| RefSeq Size | 2286 bp |
| RefSeq ORF | 1356 bp |
| Locus ID | 20459 |
| UniProt ID | Q64434 |
| Gene Summary | Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TNFa/IFN? Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells
,Haines, RJ;Beard, RS;Eitner, RA;Chen, L;Wu, MH;,
PLoS ONE
,PubMed ID 27119373
[Ptk6]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215963 | Ptk6 (untagged) - Mouse PTK6 protein tyrosine kinase 6 (Ptk6), (10ug) |
CNY 5,800.00 |
|
| MR227251 | Ptk6 (Myc-DDK-tagged) - Mouse PTK6 protein tyrosine kinase 6 (Ptk6) |
CNY 3,656.00 |
|
| MR227251L3 | Lenti ORF clone of Ptk6 (Myc-DDK-tagged) - Mouse PTK6 protein tyrosine kinase 6 (Ptk6) |
CNY 5,890.00 |
|
| MR227251L4 | Lenti ORF clone of Ptk6 (mGFP-tagged) - Mouse PTK6 protein tyrosine kinase 6 (Ptk6) |
CNY 5,890.00 |
