Apod (NM_007470) Mouse Tagged ORF Clone
CAT#: MG227214
- TrueORF®
Apod (tGFP-tagged) - Mouse apolipoprotein D (Apod), (10ug)
ORF Plasmid: DDK
"NM_007470" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227214 representing NM_007470
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACCATGCTGATGTTCCTGGCCACGCTGGCGGGTCTCTTCACCACAGCCAAAGGACAAAATTTCC ATCTTGGGAAATGCCCGTCTCCTCCTGTGCAAGAGAATTTTGACGTGAAAAAGTATCTTGGAAGATGGTA CGAAATTGAGAAGATCCCAGCGAGCTTTGAGAAAGGAAACTGCATTCAAGCCAACTACTCGCTGATGGAG AACGGAAACATCGAAGTGCTAAACAAGGAGCTGAGTCCTGATGGAACCATGAACCAAGTAAAGGGTGAAG CCAAACAGAGCAACGTCTCAGAGCCAGCCAAGCTGGAAGTCCAGTTCTTCCCGTTGATGCCACCGGCACC CTACTGGATCCTGGCCACTGATTATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTC TTCCATGTGGATTTTGTTTGGATTCTTGGAAGAAATCCTTATCTCCCTCCAGAAACAATAACCTACCTAA AAGATATCCTTACTTCTAATGGCATCGACATCGAAAAAATGACAACAACAGATCAAGCGAACTGCCCGGA CTTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227214 representing NM_007470
Red=Cloning site Green=Tags(s) MVTMLMFLATLAGLFTTAKGQNFHLGKCPSPPVQENFDVKKYLGRWYEIEKIPASFEKGNCIQANYSLME NGNIEVLNKELSPDGTMNQVKGEAKQSNVSEPAKLEVQFFPLMPPAPYWILATDYENYALVYSCTTFFWL FHVDFVWILGRNPYLPPETITYLKDILTSNGIDIEKMTTTDQANCPDFL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007470 |
ORF Size | 567 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007470.3, NP_031496.2 |
RefSeq Size | 1862 bp |
RefSeq ORF | 570 bp |
Locus ID | 11815 |
UniProt ID | P51910 |
Gene Summary | The protein encoded by this gene is a component of high-density lipoprotein (HDL), but is unique in that it shares greater structural similarity to lipocalin than to other members of the apolipoprotein family, and has a wider tissue expression pattern. The encoded protein is involved in lipid metabolism, and ablation of this gene results in defects in triglyceride metabolism. Elevated levels of this gene product have been observed in multiple tissues of Niemann-Pick disease mouse models, as well as in some tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208138 | Apod (untagged) - Mouse apolipoprotein D (Apod), (10ug) |
CNY 3,990.00 |
|
MR227214 | Apod (Myc-DDK-tagged) - Mouse apolipoprotein D (Apod) |
CNY 2,400.00 |
|
MR227214L3 | Lenti ORF clone of Apod (Myc-DDK-tagged) - Mouse apolipoprotein D (Apod) |
CNY 4,750.00 |
|
MR227214L4 | Lenti ORF clone of Apod (mGFP-tagged) - Mouse apolipoprotein D (Apod) |
CNY 4,750.00 |