Rp1 (NM_001195662) Mouse Tagged ORF Clone
CAT#: MG226963
- TrueORF®
Rp1 (tGFP-tagged) - Mouse retinitis pigmentosa 1 (human) (Rp1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001195662" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Dcdc3; Gm38717; mG145; O; Orp1; Rp; Rp1h |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226963 representing NM_001195662
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGAGAAATTGCAGGTCTCACCCAAAATGAGTGACACACCTTCTACTAGTTTCTCCATGATTCATC TGACTTCTGAAGGTCAAGTTCCTTCCCCTCGCCATTCAAATATCACTCATCCTGTAGTGGCTAAACGCAT CAGTTTCTATAAGAGTGGAGACCCACAGTTTGGCGGCGTTCGGGTGGTGGTCAACCCTCGTTCCTTTAAG ACTTTTGACGCTCTGCTGGACAGTTTATCCAGGAAGGTACCCCTGCCCTTTGGGGTAAGGAACATCAGCA CGCCCCGTGGACGACACAGCATCACCAGGCTGGAGGAGCTAGAGGACGGCAAGTCTTATGTGTGCTCCCA CAATAAGAAGGTGCTGCCAGTTGACCTGGACAAGGCCCGCAGGCGCCCTCGGCCCTGGCTGAGTAGTCGC TCCATAAGCACGCATGTGCAGCTCTGTCCTGCAACTGCCAATATGTCCACCATGGCACCTGGCATGCTCC GTGCCCCAAGGAGGCTCGTGGTCTTCCGGAATGGTGACCCGAAGAATAAGCATGTGGTCCTTCTTAGCCG GAGGATCACTCAGAGCTTCGAAGCCTTTCTGCAGTACCTGACACAGGTTATGCAGTGTCCTGTGGCCAAG CTGTATGCCACAGATGGAAGAAAAGTTCCTAGTCTCCAGGCAGTGATACTCAGCTCTGGAGCTGTGGTGG CAGCTGGAAGGGAGCCATTTAAACCAGGAAATTACGACATACAAAAGTACTTGCTTCCTGCCAAGTTACC AGGAATCTCTCATCGTGTGCACCAGAAGGGAAAAGCCAAGATAGAAAAAAGAAAGAATTATGGTTGGGGA CATAGGAACGCCGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226963 representing NM_001195662
Red=Cloning site Green=Tags(s) MLEKLQVSPKMSDTPSTSFSMIHLTSEGQVPSPRHSNITHPVVAKRISFYKSGDPQFGGVRVVVNPRSFK TFDALLDSLSRKVPLPFGVRNISTPRGRHSITRLEELEDGKSYVCSHNKKVLPVDLDKARRRPRPWLSSR SISTHVQLCPATANMSTMAPGMLRAPRRLVVFRNGDPKNKHVVLLSRRITQSFEAFLQYLTQVMQCPVAK LYATDGRKVPSLQAVILSSGAVVAAGREPFKPGNYDIQKYLLPAKLPGISHRVHQKGKAKIEKRKNYGWG HRNAV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001195662 |
ORF Size | 855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001195662.1, NP_001182591.1 |
RefSeq Size | 3047 bp |
RefSeq ORF | 858 bp |
Locus ID | 19888 |
Gene Summary | This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jun 2019] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209321 | Rp1 (untagged) - Mouse retinitis pigmentosa 1 (human) (Rp1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR226963 | Rp1 (Myc-DDK-tagged) - Mouse retinitis pigmentosa 1 (human) (Rp1), transcript variant 2 |
CNY 2,400.00 |
|
MR226963L3 | Lenti ORF clone of Rp1 (Myc-DDK-tagged) - Mouse retinitis pigmentosa 1 (human) (Rp1), transcript variant 2 |
CNY 4,800.00 |
|
MR226963L4 | Lenti ORF clone of Rp1 (mGFP-tagged) - Mouse retinitis pigmentosa 1 (human) (Rp1), transcript variant 2 |
CNY 4,750.00 |