Pld1 (NM_008875) Mouse Tagged ORF Clone
CAT#: MG226799
- TrueORF®
Pld1 (tGFP-tagged) - Mouse phospholipase D1 (Pld1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_008875" in other vectors (5)
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CNY 9,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA536939; C85393; mPLD1; Pld1a; Pld1b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226799 representing NM_008875
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACTGAAAAGCGAGACCAGGGTGAACACGTCTACACTGCAGAAAATCGCCGCAGATATGAGCAACC TGATAGAAAATCTCGACACCCGGGAGCTTCATTTTGAGGGAGAGGAGGTGGAATATGACGCATCTCCAGG AGACCCCAAGGCCCAGGAAGGGTGCATCCCATTCTCTTCTATTTACAACACTCAAGGATTTAAGGAGCCC AACATCCAGACCTACCTCTCTGGCTGTCCCATAAAAGCACAAGTACTGGAAGTGGAGCGCTTCACATCGA CCTCAAGGGTGCCAAGTATCAACCTGTACACGATCGAGTTAACGCACGGGGAATTCACATGGCAAGTTAA GAGGAAATTCAAGCACTTCCAAGAGTTTCACAGAGAGCTGCTCAAGTACAAAGCCTTCATCCGGATCCCC ATCCCCACTAAAAGACATACATTTAGAAGACAAAATGTCAAAGAAGAACCCCGAGAGATGCCCAGTCTGC CCCGTTCATCAGAAAACGCAATCCAGGAAGAGCAGTTCTTTGGCAGGAGGAAGCAACTGGAAGATTACTT GACAAAAATACTAAAAATGCCCATGTACAGAAACTATCATGCCACGACAGAGTTCCTTGATGTAAGCCAG CTGTCCTTCATCCATGATCTGGGACCAAAGGGCCTGGAAGGAATGATCATGAAAAGGTCTGGGGGACACA GGATACCAGGTGTGAATTGCTGTGGCCATGGACGAGCCTGCTACCGGTGGTCAAAGAGGTGGTTGATAGT AAAAGACTCTTTCCTGCTCTATATGAAACCAGATAGCGGGGCTATTGCCTTCGTCCTCCTTGTAGATAAA GAATTCAGAGTCAAAGTGGGAAGGAAAGAGACAGAAACGAAATATGGACTTCGGATCGATAATCTTTCAA GGACCCTGATTTTAAAATGTAACAGCTACAGACACGCCCGGTGGTGGGGAGGCGCCATCGAGGAGTTCAT CCGGAAGCATGGAGCTGACTTCCTCAAAGACCATCGCTTCGGGTCCTACGCCGCTCTCCATGAGAACACG TTAGCTAAGTGGTATGTGAACGCCAAAGGTTACTTTGAAGACATCGCAAATGCAATGGAAGAGGCATCGG AAGAGATTTTTATCACAGATTGGTGGTTAAGTCCAGAAATCTTCCTGAAGCGCCCAGTGGTGGAAGGCAA CCGCTGGAGGCTGGACTGCATCCTCAAACGGAAAGCACAACAAGGTGTGCGGATCTTCATAATGCTTTAC AAAGAGGTGGAGCTAGCCCTCGGGATCAACAGCGAGTACAGCAAGCGGACGTTGATGAGGCTGCACCCCA ACATAAAGGTGATGAGACACCCAGACCACGTGTCCTCCAGCGTGTACCTGTGGGCTCACCATGAGAAGCT TGTCATTATCGACCAATCAGTGGCTTTCGTGGGTGGGATTGACCTGGCCTACGGAAGGTGGGATGACAAT GAGCACAGACTCACAGACGTGGGCAGTGTCAAGCGGGTCACCTCAGGACTGTCTCTGGGCTCCCTCACGG CAGCATCAGTAGAGTCTATGGAATCCTTAAGCCTCAAGGATAAACATGAATTTCATAAAAAGGAGCCCAT CTCAAAGATTGTTGATGAGACTGACATGAAGCTGAAAGGGATAGGAAAGTCCAGGAAATTCTCCAAATTT AGTCTCTATCGCCAGCTGCACCGTCACCACCTGCACAACGCGGACAGCATCAGCAGCATCGACAGCACCT CCAACACTGGCTCCATCCGCAGCGTGCAGACCGGAGTGGGAGAACTCCACGGGGAGACTAGGTTTTGGCA TGGGAAGGATTACTGCAACTTTGTCTTCAAGGACTGGGTTCAACTGGACAAACCTTTTGCTGATTTCATT GACAGGTACTCAACCCCCCGGATGCCTTGGCATGATATTGGCTCTGTGGTCCATGGAAAGGCAGCTCGGG ATGTGGCTCGACACTTCATCCAGCGCTGGAACTTCACGAAGATTATGAAGCCAAAATACCGCTCCCTTTC TTATCCCTTCCTGCTACCAAAGTCTCAAGCGACAGCACACGAGTTGAGATATCAGGTGCCAGGGGCCGTC CCCGCCAAAGTGCAGTTGCTCCGATCTGCTGCTGATTGGTCTGCTGGTATAAAGCACCATGAGGAATCCA TCCACGCTGCCTACATCCATGTAATAGAGAACAGCAAGCACTACATCTATATTGAAAACCAATTTTTCAT AAGCTGTGCTGATGATAAAGTTGTGTTCAACAAGGTGGGAGATGCTATCGCCCAGAGAATCCTGAAAGCC CACAGGGAAGGCCAGAGATATCGGGTGTACATTGTGATACCGCTGCTGCCGGGATTCGAGGGAGACATTT CCACAGGCGGGGGGAACGCTCTACAGGCAATAATGCACTTCAACTACAGAACCATGTGCAGAGGGGAGAG CTCCATCCTTGAACAGTTAAAGCCAGAGCTTGGTAATAAGTGGATAAATTACATATCGTTTTGTGGACTG AGAACACATGCAGAGCTGGAAGGAAACCTAGTCACCGAGCTCATCTACGTCCACAGCAAGTTGTTAATTG CTGACGACAACACAGTTATTATTGGTTCTGCCAACATCAATGACCGGAGCATGCTGGGGAAGCGAGACAG CGAAATGGCTGTCATAGTGCAGGACACAGAGACGGTCCCATCGGTGATGGATGGAAAGGAATATCAGGCT GGTCGCTTTGCCCGAGACCTTCGCCTGGAGTGCTTCAGACTTGTCCTGGGTTATCTTTCTGACCCAAGTG AGGACCTTCAGGATCCTGTGAGTGACAAATTCTTCAAGGAGATTTGGGTTTCAACAGCAGCTCGAAACGC TACCATATATGATAAGGTCTTCCGGTGCCTTCCCAATGATGAAGTACACAATTTAATCCAGCTGCGGGAC TTTATAAACAAGCCCATATTAGCAAAAGAGGATCGCCTTCGCGCCGAGGAAGAACTGAGGAAGATCCGCG GGTTCTTGGTGCAGTTCCCTCTCTATTTCTTATCAGAAGAGAACCTGCTGCCTTCTGTTGGAACCAAAGA AGCCATTGTGCCTATGGAGGTTTGGACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226799 representing NM_008875
Red=Cloning site Green=Tags(s) MSLKSETRVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPKAQEGCIPFSSIYNTQGFKEP NIQTYLSGCPIKAQVLEVERFTSTSRVPSINLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIP IPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQ LSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDK EFRVKVGRKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIRKHGADFLKDHRFGSYAALHENT LAKWYVNAKGYFEDIANAMEEASEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLY KEVELALGINSEYSKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDN EHRLTDVGSVKRVTSGLSLGSLTAASVESMESLSLKDKHEFHKKEPISKIVDETDMKLKGIGKSRKFSKF SLYRQLHRHHLHNADSISSIDSTSNTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFI DRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAV PAKVQLLRSAADWSAGIKHHEESIHAAYIHVIENSKHYIYIENQFFISCADDKVVFNKVGDAIAQRILKA HREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGL RTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQA GRFARDLRLECFRLVLGYLSDPSEDLQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRD FINKPILAKEDRLRAEEELRKIRGFLVQFPLYFLSEENLLPSVGTKEAIVPMEVWT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008875 |
ORF Size | 3108 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008875.4, NP_032901.2 |
RefSeq Size | 4932 bp |
RefSeq ORF | 3111 bp |
Locus ID | 18805 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223273 | Pld1 (untagged) - Mouse phospholipase D1 (Pld1), transcript variant 2, (10ug) |
CNY 13,300.00 |
|
MG211495 | Pld1 (tGFP-tagged) - Mouse phospholipase D1 (Pld1) |
CNY 9,220.00 |
|
MR226799 | Pld1 (Myc-DDK-tagged) - Mouse phospholipase D1 (Pld1), transcript variant 2 |
CNY 7,224.00 |
|
MR226799L3 | Lenti ORF clone of Pld1 (Myc-DDK-tagged) - Mouse phospholipase D1 (Pld1), transcript variant 2 |
CNY 10,260.00 |
|
MR226799L4 | Lenti ORF clone of Pld1 (mGFP-tagged) - Mouse phospholipase D1 (Pld1), transcript variant 2 |
CNY 10,260.00 |