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Dact1 (NM_021532) Mouse Tagged ORF Clone
CAT＃: MG226688
- TrueORF®
Dact1 (tGFP-tagged) - Mouse dapper homolog 1 antagonist of beta-catenin (xenopus) (Dact1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_021532" in other vectors (4)
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CNY 8,550.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921528D17Rik; AI115603; DAPPER; DAPPER1; Frd1; FRODO; Frodo1; MDpr1; MTNG3; THYEX3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226688 representing NM_021532
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGGACGCAGCGCGCGAGCCGGAGCCGCTGAGCCCCGGCCGGGGCGCGGAGGCCGAGGGGCGCT GGCGCGAGAGGGGCGAGGCGGACACGGAGCGGCAGCGTACCCGCGAGCGCCAGGAGGCCACGCTGGCGGG GCTGGCGGAGCTGGGGTACCTGCGGCAACGCCAAGAGCTGCTGGTGCGCGGTGCGCTGCGCTGCTCCGGG ACCGTGGGGACCGTCGCGCCGCGCTCCGGGGAGCTGCGGGGAGACGCGGCGCAGCGCAGCCGCCTGGAGG AGAAGTTCCTGGAAGAGAACATCTTGCTGCTGCGAAGGCAGTTGAATTGTTTGAGGAGAAGAGATGCCGG TTTGTTGAATCAGTTGCAAGAACTTGACAAGCAGATAAGTGACCTGAGACTGGATGTGGAGAAGACATCT GAAGAGCACCTGGAGACAGACAGCCGGCCTAGCTCAGGGTTTTATGAGCTGAGTGATGGAGCTTCGGGCT CCCTCTCTAACTCCTCCAACTCCGTGTTCAGTGAGTGTTTGTCCAGTTGCCATTCCAGCACCTGCTTCTG CAGCCCCTTGGAGGCGGCCTTGACCATCTCAGACGGTTGCCCCAAATCTGCAGATGTGAATCCTAAATAC CAGTGTGATCTTGTGTCTAAAAACGGGAATGACGTATATCGCTACCCCAGTCCACTTCATGCTGTGGCTG TGCAGAGCCCAATGTTTCTCCTTTGTCTGACGGGCAACACTCTGAGGGAAGAGGAGGGGCTTGGGAGCCA TGCCAGCGACATCTGCATTGGATCTGAACTGAACGCCACCAAAACAGACAATTCCCTGCCATCTCCAAGC AGTTTGTGGTCCGCTTCCCATCCTGCATCCAGTAAGAAAATGGATGGGTATATTTTGAGCCTCGTGCAGA AGAAAACACACCCTGTAAGGACCAATAAACCTAGAACCAGTGTGAACGCTGACCCTACCAAGGGCCTTCT GAGGAATGGAAGTGTGTGTGTCAGGGCCCCTAGTGGCGTCCCACCGGGCAGTAGTGTGAACTTTAAGAAT ACAAAACAGATGTGTTTGCCCGCTGGGGGAATAACCTCTTTGGAAAACGGGCCATTCTCCCCTCCTAAGC AGAGGTCCAAAGACTCAAAGACAGACCAGTTAGAAAGCAAGAGGTTGGCTCTGCCGGAGAGCTGCTCGGC AGGCGCCGCCATGGAACCCCAAAGCAAGCATGTGCCCAAAGCCGCCAAGGCAGCCTCTCAAGAGCTCACA AGGTGTCAAGCCGGGCTGGGGGAATCCATGAAGGAAAGCAATCAGGCCTCCGCTGTTTCTCCTAAAACAA GTCCTGGCAGAGGCCCTGTCGCCCCCGCAGAGAGCAAAGCCCTGCAGCTCCCGAAAAAGATGTCGCAGAA GAACAGCCTCCAGGCTGTGCCCGCGCTGGACAGGCCGGCCTTGGACTTCAAAAGCGAGGGCTCATCTCAA AGCCTCGAGGAAGGGCATCTGGTGAAAGCTCAGTTCATTCCGGGGCAGCAGGCGGCCGCCAGGCCTCACC GTGCACACAGGAACCCGGGTGTCGCAAGGAGCGCCACCTTGAAGGCCCGCGGCCAGGCAGCCATGGAACA CGGCCTGCCCACCGTCAGGGAGAAACCGCGGACAGCAGGCAAGAAGTGCCGTTTCCCAGACGACTCGGAT ACAAATAAGAAATTCAGGAAGACCTCCGCCAAGGGCCGGCGCAGTGGCGGGCTGCAGGACGCTGGCCTTC CCGGTAGGGCCCTGGGCACCGGCGGCCATCGGGCGGGTAGCAGGGCGCACGCGCATGGCCGGGAGCCCGT GGTGGCCAAACCGAAGCACAAGCGAACCGACTACCGGCGGTGGAAATCGTCAGCCGAGGTCTCCTACGAA GAAGCCCTGCGGAGGGCCCGGAGGGCTCGCAGGGAGCACGGGGCTGCCTACCCGGTGGCTGTCGCCCTGC CTTACGCCAGCCCCTATGCCTACGTGCCCAGCGACTCCGAGTACTCGGCGGAGTGCGAGTCGCTCTTCCA CTCCACGGTGGTGGACACCAGCGAGGACGAGCAGAGCAACTACACCACCAACTGCTTCGGCGACAGCGAG TCCAGCGTGAGCGAAGGCGACTTCGTGGGCGAGAGCACCACCACCAGCGACTCAGAGGAGAGCGGGGGTT TAATCTGGTCCCAGTTTGTCCAGACTCTCCCGATTCAAGCGGTCACGGCCCCAGACCTCCACACCCGTCC CACAAAAACCTTTGTCAAAATCAAGGCTTCGCACAACCTCAAGAAGAAGATCCTCCGTTTCCGCTCTGGC TCTTTGAAACTGATGACTACCGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226688 representing NM_021532
Red=Cloning site Green=Tags(s) MKPDAAREPEPLSPGRGAEAEGRWRERGEADTERQRTRERQEATLAGLAELGYLRQRQELLVRGALRCSG TVGTVAPRSGELRGDAAQRSRLEEKFLEENILLLRRQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTS EEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEAALTISDGCPKSADVNPKY QCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNTLREEEGLGSHASDICIGSELNATKTDNSLPSPS SLWSASHPASSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPSGVPPGSSVNFKN TKQMCLPAGGITSLENGPFSPPKQRSKDSKTDQLESKRLALPESCSAGAAMEPQSKHVPKAAKAASQELT RCQAGLGESMKESNQASAVSPKTSPGRGPVAPAESKALQLPKKMSQKNSLQAVPALDRPALDFKSEGSSQ SLEEGHLVKAQFIPGQQAAARPHRAHRNPGVARSATLKARGQAAMEHGLPTVREKPRTAGKKCRFPDDSD TNKKFRKTSAKGRRSGGLQDAGLPGRALGTGGHRAGSRAHAHGREPVVAKPKHKRTDYRRWKSSAEVSYE EALRRARRARREHGAAYPVAVALPYASPYAYVPSDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSE SSVSEGDFVGESTTTSDSEESGGLIWSQFVQTLPIQAVTAPDLHTRPTKTFVKIKASHNLKKKILRFRSG SLKLMTTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021532 |
| ORF Size | 2334 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021532.4, NP_067507.2 |
| RefSeq Size | 3650 bp |
| RefSeq ORF | 2337 bp |
| Locus ID | 59036 |
| UniProt ID | Q8R4A3 |
| Gene Summary | Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB (By similarity). Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling (By similarity). May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221495 | Dact1 (untagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 2, (10ug) |
CNY 9,980.00 |
|
| MR226688 | Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 2 |
CNY 7,790.00 |
|
| MR226688L3 | Lenti ORF clone of Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 2 |
CNY 9,690.00 |
|
| MR226688L4 | Lenti ORF clone of Dact1 (mGFP-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 2 |
CNY 9,690.00 |
