Nrxn1 (NM_020252) Mouse Tagged ORF Clone
CAT#: MG226542
- TrueORF®
Nrxn1 (tGFP-tagged) - Mouse neurexin I (Nrxn1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_020252" in other vectors (4)
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CNY 13,300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1700062G21Rik; 9330127H16Rik; A230068P09Rik; mKIAA0578 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226542 representing NM_020252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGGCGCTGGTCCAGCGCGGGGGCTGCTGTCTCCTCTGCCTGTCGCTGCTGCTGCTGGGCTGCT GGGCAGAGCTGGGCAGCGGGCTGGAGTTCCCGGGCGCCGAGGGCCAGTGGACGCGCTTCCCCAAGTGGAA CGCGTGCTGCGAGAGCGAGATGAGCTTCCAGCTGAAGACGCGCAGTGCCCGCGGCCTCGTGCTCTACTTC GACGACGAGGGCTTCTGCGACTTCCTCGAGCTCATCCTGACGCGCGGCGGCCGCCTGCAGCTCAGCTTCT CCATCTTCTGCGCCGAGCCCGCCACGCTGTTGGCCGACACGCCGGTCAACGACGGCGCCTGGCACAGCGT GCGCATCCGCCGCCAGTTCCGCAACACCACGCTCTACATCGACCGCGCCGAGGCCAAGTGGGTGGAGGTC AAGTCCAAGCGCAGGGACATGACGGTGTTCAGCGGGCTCTTCGTGGGCGGCCTGCCACCCGAACTGCGCG CCGCGGCCCTCAAGCTCACGCTGGCCTCGGTGCGCGAGCGCGAGCCCTTCAAGGGCTGGATCCGTGACGT CCGGGTCAACTCGTCACAGGCCCTGCCGGTGGACGGCGGCGAGGTCAAGCTGGACGACGAGCCTCCCAAC AGCGGTGGCGGGAGCCCATGCGAGGCGGGCGAAGAAGGCGAGGGCGGCGTGTGCCTCAACGGGGGCGTGT GCTCCGTGGTCGACGACCAGGCCGTATGCGACTGCTCGAGGACCGGCTTCCGTGGCAAGGACTGCAGCCA AGAAGACAACAATGTGGAAGGTCTGGCACACCTGATGATGGGCGACCAAGGTAAAAGTAAAGGAAAAGAA GAGTATATTGCCACGTTCAAGGGATCTGAATACTTCTGCTACGACTTATCTCAAAACCCCATTCAAAGCA GCAGTGATGAAATAACTCTGTCCTTTAAAACTCTTCAAAGGAACGGACTGATGCTTCACACAGGGAAATC AGCTGATTATGTCAATCTTGCATTGAAAAATGGAGCTGTCTCTCTGGTCATTAATTTGGGATCAGGGGCC TTTGAAGCACTAGTGGAGCCTGTGAATGGAAAGTTTAATGATAATGCCTGGCATGATGTGAAAGTCACCA GGAATCTGCGTCAGCACTCAGGCATTGGACACGCTATGGTGACAATATCAGTGGATGGAATTCTTACCAC AACGGGCTACACGCAAGAAGATTACACCATGCTGGGGTCTGATGACTTTTTCTATGTTGGAGGCAGCCCC AGCACAGCCGACCTTCCAGGGTCACCAGTCAGTAACAACTTTATGGGCTGTCTCAAAGAGGTTGTATATA AAAATAATGATGTAAGGCTGGAATTATCGCGACTTGCCAAGCAAGGAGATCCTAAGATGAAGATTCACGG TGTGGTGGCTTTTAAGTGTGAAAATGTGGCAACCTTAGACCCAATAACTTTTGAGACCCCGGAATCTTTC ATTTCTTTGCCTAAGTGGAATGCAAAGAAAACAGGCTCAATATCATTTGATTTCCGTACAACGGAGCCAA ATGGCCTCATCCTGTTTAGCCATGGCAAGCCAAGACATCAGAAGGATGCCAAGCACCCACAGATGATTAA GGTTGACTTTTTTGCTATTGAGATGCTGGATGGCCACTTGTACCTCCTCTTAGACATGGGATCAGGTACT ATAAAAATCAAAGCCCTGCAGAAGAAAGTCAATGATGGAGAATGGTATCACGTGGATTTCCAACGGGATG GACGGTCAGGTACCATTTCTGTCAACACACTACGCACTCCCTACACAGCTCCCGGTGAGAGTGAGATCCT GGACCTAGATGATGAGTTGTACCTGGGTGGCTTGCCAGAAAATAAGGCTGGCCTAGTCTTCCCTACCGAG GTGTGGACTGCTCTGCTCAACTACGGCTACGTGGGCTGCATCCGGGACCTATTTATTGATGGCCAGAGCA AAGACATCCGGCAGATGGCGGAAATTCAGAGCACTGCTGGAGTGAAGCCATCCTGCTCAAAAGAGACAGC AAAACCGTGCCTTAGCAATCCTTGCAAAAACAATGGCATGTGCAGAGATGGCTGGAACAGATACGTCTGC GATTGCTCCGGAACAGGCTATCTCGGCAGGTCCTGTGAGAGAGAGGCAACGGTTTTGAGCTACGACGGGA GCATGTTCATGAAAATCCAGCTTCCGGTGGTGATGCACACTGAGGCTGAGGACGTGTCCTTGCGGTTCCG ATCCCAGCGTGCATATGGTATCCTGATGGCGACCACTTCTAGAGACTCTGCAGACACCCTTCGATTGGAG CTAGATGCAGGACGTGTGAAACTCACGGTCAATCTAGATTGTATCAGGATTAACTGTAATTCCAGCAAAG GTCCTGAGACCCTTTTTGCTGGCTATAACCTCAATGATAATGAATGGCACACAGTGCGTGTGGTTCGGCG AGGAAAAAGTTTAAAGCTAACCGTGGATGATCAACAAGCCATGACAGGCCAAATGGCAGGTGATCATACA AGGCTGGAGTTTCATAACATAGAGACAGGCATCATCACCGAACGACGCTACCTTTCTTCTGTCCCCTCCA ATTTCATTGGGCACCTGCAAAGCCTGACATTTAATGGAATGGCATACATAGACTTGTGCAAAAATGGTGA TATAGATTACTGTGAACTCAATGCCAGGTTTGGATTCAGGAACATCATCGCAGATCCTGTCACCTTCAAG ACCAAATCGAGCTATGTTGCCTTAGCTACATTGCAAGCCTACACCTCTATGCATCTATTTTTCCAATTCA AGACAACATCTCTAGATGGACTAATTCTGTATAACAGTGGGGATGGAAATGACTTTATTGTGGTTGAATT AGTTAAAGGGTACTTACATTATGTGTTTGACTTGGGAAACGGTGCTAACCTCATCAAAGGGAGCTCAAAC AAACCACTCAACGACAATCAGTGGCACAATGTGATGATTTCACGGGACACCAGCAATCTCCACACAGTAA AGATCGACACAAAAATCACAACACAAATCACTGCGGGAGCCAGAAATCTAGACCTCAAGAGTGACTTGTA TATTGGAGGAGTGGCTAAAGAAACATATAAATCCCTGCCAAAACTGGTCCATGCCAAAGAAGGCTTTCAA GGTTGCCTGGCATCTGTGGACTTGAATGGCAGACTTCCAGATCTCATCTCGGATGCTCTCTTCTGCAATG GGCAAATTGAGAGAGGATGCGAAGGGCCCAGCACAACCTGCCAAGAGGATTCATGTTCAAATCAAGGCGT TTGCTTGCAGCAATGGGATGGCTTCAGCTGTGACTGTAGCATGACTTCCTTCAGTGGCCCACTCTGCAAT GACCCTGGGACAACATATATCTTTAGCAAAGGTGGTGGACAGATTACATATAAGTGGCCTCCCAATGACC GCCCCAGTACACGAGCAGACAGGCTGGCCATCGGATTTAGCACTGTCCAGAAGGAAGCCGTGTTGGTGCG TGTGGACAGTTCCTCAGGACTGGGTGACTACCTGGAGCTGCACATACACCAAGGAAAAATTGGAGTTAAG TTTAATGTTGGGACAGATGACATCGCCATCGAAGAGTCTAATGCAATCATTAATGATGGGAAATACCATG TAGTACGTTTCACAAGGAGTGGTGGCAATGCCACGTTACAGGTGGACAGCTGGCCAGTTATCGAACGCTA CCCTGCAGGAAACAATGATAACGAGCGCCTGGCGATTGCTAGACAGCGAATTCCATATCGACTTGGTCGA GTAGTTGATGAATGGCTACTCGACAAAGGGCGTCAGCTCACAATCTTCAATAGCCAAGCAACCATAATAA TTGGCGGGAAAGAGCAGGGCCAGCCCTTCCAGGGCCAGCTCTCTGGTCTTTACTACAATGGCTTGAAAGT TCTGAATATGGCAGCAGAGAACGATGCCAACATCGCCATAGTGGGGAATGTGAGGCTGGTCGGTGAAGTG CCTTCCTCTATGACAACTGAGTCGACAGCCACTGCCATGCAGTCTGAGATGTCCACCTCAATCATGGAGA CCACCACAACCCTGGCTACCAGCACAGCTAGAAGAGGCAAGCCCCCCACAAAGGAACCCATCAGCCAGAC CACAGATGACATCCTTGTGGCCTCGGCAGAGTGTCCCAGTGACGATGAGGACATTGACCCCTGTGAGCCG AGCTCAGGTGGGTTAGCCAACCCCACCAGAGTAGGTGGCCGTGAACCATACCCAGGCTCTGCAGAGGTGA TTCGGGAGTCCAGCAGTACCACTGGCATGGTGGTGGGGATTGTCGCAGCAGCCGCTCTGTGCATCCTCAT CCTCCTCTATGCCATGTACAAGTACAGGAACCGGGATGAAGGGTCGTACCACGTGGATGAGAGTCGAAAC TACATCAGTAACTCAGCACAGTCCAATGGGGCTGTGGTCAAGGAGAAGCAACCCAGCAGTGCTAAAAGCG CCAACAAAAACAAGAAAAACAAGGATAAGGAGTATTACGTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226542 representing NM_020252
Red=Cloning site Green=Tags(s) MGTALVQRGGCCLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPKWNACCESEMSFQLKTRSARGLVLYF DDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIRRQFRNTTLYIDRAEAKWVEV KSKRRDMTVFSGLFVGGLPPELRAAALKLTLASVREREPFKGWIRDVRVNSSQALPVDGGEVKLDDEPPN SGGGSPCEAGEEGEGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKE EYIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGA FEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSP STADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQGDPKMKIHGVVAFKCENVATLDPITFETPESF ISLPKWNAKKTGSISFDFRTTEPNGLILFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGT IKIKALQKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAGLVFPTE VWTALLNYGYVGCIRDLFIDGQSKDIRQMAEIQSTAGVKPSCSKETAKPCLSNPCKNNGMCRDGWNRYVC DCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHTEAEDVSLRFRSQRAYGILMATTSRDSADTLRLE LDAGRVKLTVNLDCIRINCNSSKGPETLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHT RLEFHNIETGIITERRYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFK TKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGANLIKGSSN KPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGGVAKETYKSLPKLVHAKEGFQ GCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCN DPGTTYIFSKGGGQITYKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVK FNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGR VVDEWLLDKGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEV PSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEP SSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRN YISNSAQSNGAVVKEKQPSSAKSANKNKKNKDKEYYV SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020252 |
ORF Size | 4521 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020252.3, NP_064648.3 |
RefSeq Size | 9040 bp |
RefSeq ORF | 4524 bp |
Locus ID | 18189 |
UniProt ID | Q9CS84 |
Gene Summary | This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are synaptic transmembrane receptors that bind endogenous ligands that include neuroligins, dystroglycan, and neurexophilins. Neurexin complexes are required for efficient neurotransmission and are involved in synaptogenesis. In vertebrates, alternate promoter usage results in multiple isoform classes, of which the alpha and beta classes are the best characterized. In humans, allelic variants in this gene are associated with Pitt-Hopkins-like syndrome-2, while deletions have been associated with autism and schizophrenia. Mouse knockouts display decreased spontaneous and evoked vesicle release resulting in impaired synaptic transmission. In addition, knockout mice show altered social approach, reduced social investigation, reduced locomotor activity, and in males, increased aggression. Alternative splicing and promoter usage result in multiple transcript variants. [provided by RefSeq, Nov 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224615 | Nrxn1 (untagged) - Mouse neurexin I (Nrxn1), transcript variant 1, (10ug) |
CNY 19,290.00 |
|
MR226542 | Nrxn1 (Myc-DDK-tagged) - Mouse neurexin I (Nrxn1), transcript variant 1 |
CNY 10,312.00 |
|
MR226542L3 | Lenti ORF clone of Nrxn1 (Myc-DDK-tagged) - Mouse neurexin I (Nrxn1), transcript variant 1 |
CNY 13,970.00 |
|
MR226542L4 | Lenti ORF clone of Nrxn1 (mGFP-tagged) - Mouse neurexin I (Nrxn1), transcript variant 1 |
CNY 13,970.00 |