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Usp37 (NM_176972) Mouse Tagged ORF Clone
CAT＃: MG226485
- TrueORF®
Usp37 (tGFP-tagged) - Mouse ubiquitin specific peptidase 37 (Usp37), (10ug)
ORF Plasmid: DDK
"NM_176972" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4932415L06Rik; C330008N13Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226485 representing NM_176972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCCCTGAAGATCTATGGTCCTATCAGAATTCGAAGCATGCAGACTGGGATTACAAAGTGGAAAG AAGGGTCCTTTGAAATTGTGGAAAAAGACAATAGAGTCAGCCTGCTCGTTCACTACAACACTGGAGGAAT TCCAAGAGTGTTTCAGCTAAGTCATAACATTAAAAATGTGGTACTACGACCCAGTGGAATAAAACAAAGC CGCCTAATGTTGACTTTACAAGATAACAGCTTCTTGTCTATTGACAAAGTACCAAGTAAGGATGCAGAGG AAATGAGGTTGTTCCTAGATGCCGTCCATCAAAACAGACTTCATGCAGCTATGAAAGCTTCTCAAGGTTC TGGTAGTTTTGGAACTATTCTGGGCAGCAGGACCTCTCAGAAGGAAACTAACAGACAGCTTTCTTACTCA GACAATCAGGCCTCTTCAAAAAGAGGAAGTTTAGAAACTAAAGATGAAATTCCATTTCGAAAAGTTCTTG GCAGTCCTGGTAGAGGACCAATTAAGACTGTAACTGGAGGTGGAATGGCTGTTACCCGGACTATTCCTTC TTTGACGCTGACATCAACACCTCTTAGATCAGGATTATTAGAAAACCGCACTGAAAAGAGGAAAAGAATG TTATCTGGTTCAGAGCTGACTGAAGATTACCCTAAGGAAAATGACTCATCATCGAACAACAAAGCTATGA CAGACCCTTCAAGAAAATATTTAACCAGCTGTAGAGAAAAGCAGCTGAGTTTGAAACAGGCAGAAGAAAA TCGAACATCAGGGCTTTTACCTCTGCAGTCATCCTCATTCTATGGGAGCAGAGCTGGATCAAAGGACTAC TCTTCTGGTGTCACTAACCTGGACAGGTGTAATGTTTCAAGCCAGACTCCTTCTGCCAAAAGAAGCCTGG GGTTTCTTCCTCAGCCAACTCCTCTGTCTGTTAAAAAACTGAGGTGTAACCAGGATTACGCGGGCTGGAA CAGACCTAGAGTGCCCCTTTCCTCCCATCAACAGCAGCTGCAGGGATTCTCCAACTTGGGAAATACATGC TATATGAATGCTATCTTACAATCTCTGTTTTCACTCCAGTCATTTGCAAATGATTTGCTTAAACAAAGTA TCCCATGGAAGAAAATTCCATTCAATGCACTTATCAGACGCTTTGCAAACCTGCTTATTAAAAAAGATAT TTGTAATTCAGAGACCAAAAAAGAACTACTTAAGAAGGTTAAAAATGCTATTTCAGCTACTGCAGAGAGA TTCTCTGGCTATGTCCAAAATGATGCTCATGAATTTTTAAGTCAGTGCTTAGATCAGCTAAAGGAGGATA TGGAAAAACTGAATAAAACTTGGAAGACAGAACCTGTCCTTGGAGAAGAAAATTTGCCAGATACATCAGC TACCAAAGTGTTCACGTGCCCTGTTATTACAAACTTGGAGTTTGAAGTTCAGCACTCCATTATCTGTAAA GCATGTGGAGAAACTATTCCCAAAAGAGAACAGTTCAATGACCTCTCCATTGACCTTCCTCGGAGGAAAA AGCCACTCCCTCCTCGTTCAATCCAAGATTCTCTCGATCTTTTCTTTAGGGCAGAAGAACTTGAATACTC ATGTGAAAAGTGTGGTGGAAAATGTGCTCTTGTCAGGCACAAATTTAACAGGCTGCCCAGGGTCCTCATC CTTCATTTGAAGCGATACAGCTTCAATGTGGCTCTCTCACTTAACAACAAGCTCGGACAGCAAGTCATCA TCCCAAGATTCCTGACCCTGGCCTCCCACTGCACTGAGAGCACAAAGCCGCCGGTCACCCTTGGGTGGAG CGCACCCGTGGCAATTTCTAGACCATTGAGAGCCTGTCAAATGGTGAATTCCTGCATCACCAGCCCTTCT GCACCTTCAAAGAAATTCACCTTCAAATCCAAGAGCTCCGTGACATCATGCCTTGATTCAGACAGTGAGG ATGAACTTAAACGCTCTGTGGTCCTCAGTCAGAGACTTTGTGATTTGCCAGGCAATGAACAGTACCAGGA AGATGTGGAAAAAGATTTGAAATTGTGCAGATTAGAGCCTGGGAAGGCAGAACTGGAGAACTCAGGATTT GACAGAATGAGTGAGGAAGAGGTGCTGGCAGCTGTGTTAGAGATAAGCAGGAGAGAAGCTTCGCCGGTAC TTAGCCCTGAAGATGATGATAAGCCAACCAGCAGTCCGGACACCGGCTTTGCAGAAGATGATATTCCAGA AATGCCTGAAAATCCAGATGCTATGGAAATTGAGAAGTCCAAAACAATCACTGAGCCAGGTCCTGCTAGT TTTACTGAGATAACAAAAGACTGTGATGAGAATAAAGAAAACAAAACTCCAGAAGGCTCTCAGGGAGAGG TTGATTGGCTCCAGCAGTATGACGTAGACCGGGAAAGAGAAGAGCAGGAGCTGCAGCAAGCACTGGCTCA GAGCCTTCAAGAGCAAGAGGCTTGGGAACAGAAGGAAGATGACGACCTCAAAAGGGCTACAGAATTAAGT CTTCAAGAGTTTAACAACTCTTTTCTGGATTCCCTGGGCTCAGATGAGGACTCTGGAAATGAGGATGTTT TTGATATGGAGTACACAGAAGCTGAAGCTGAGGAGCTGAAAAGAAATGCTGAGACAGGAGCTCTTCCTCA TTCCTATCGGCTCATCAGTGTTGTCAGTCACATTGGGAGCACTTCTTCCTCAGGTCATTACATTAGTGAT GTGTATGACATTAAGAAACAGGCATGGTTCACTTACAATGACCTGGAGGTCTCTAAAATCCAAGAGGCTG CCGTGCAGAGTGATCGAGATCGCAGCGGCTATATCTTCTTCTATATGCACAAGGAGATCTTTGATGAGTT GCTGGAGACAGAAAAAACCTCTCAGGCGCTTAGCATGGAGGTGGGGAGAGCTGCCCGTCAGGCTTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226485 representing NM_176972
Red=Cloning site Green=Tags(s) MSPLKIYGPIRIRSMQTGITKWKEGSFEIVEKDNRVSLLVHYNTGGIPRVFQLSHNIKNVVLRPSGIKQS RLMLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLHAAMKASQGSGSFGTILGSRTSQKETNRQLSYS DNQASSKRGSLETKDEIPFRKVLGSPGRGPIKTVTGGGMAVTRTIPSLTLTSTPLRSGLLENRTEKRKRM LSGSELTEDYPKENDSSSNNKAMTDPSRKYLTSCREKQLSLKQAEENRTSGLLPLQSSSFYGSRAGSKDY SSGVTNLDRCNVSSQTPSAKRSLGFLPQPTPLSVKKLRCNQDYAGWNRPRVPLSSHQQQLQGFSNLGNTC YMNAILQSLFSLQSFANDLLKQSIPWKKIPFNALIRRFANLLIKKDICNSETKKELLKKVKNAISATAER FSGYVQNDAHEFLSQCLDQLKEDMEKLNKTWKTEPVLGEENLPDTSATKVFTCPVITNLEFEVQHSIICK ACGETIPKREQFNDLSIDLPRRKKPLPPRSIQDSLDLFFRAEELEYSCEKCGGKCALVRHKFNRLPRVLI LHLKRYSFNVALSLNNKLGQQVIIPRFLTLASHCTESTKPPVTLGWSAPVAISRPLRACQMVNSCITSPS APSKKFTFKSKSSVTSCLDSDSEDELKRSVVLSQRLCDLPGNEQYQEDVEKDLKLCRLEPGKAELENSGF DRMSEEEVLAAVLEISRREASPVLSPEDDDKPTSSPDTGFAEDDIPEMPENPDAMEIEKSKTITEPGPAS FTEITKDCDENKENKTPEGSQGEVDWLQQYDVDREREEQELQQALAQSLQEQEAWEQKEDDDLKRATELS LQEFNNSFLDSLGSDEDSGNEDVFDMEYTEAEAEELKRNAETGALPHSYRLISVVSHIGSTSSSGHYISD VYDIKKQAWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMHKEIFDELLETEKTSQALSMEVGRAARQAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_176972 |
| ORF Size | 2937 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_176972.4, NP_795946.2 |
| RefSeq Size | 7196 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 319651 |
| UniProt ID | Q8C0R0 |
| Gene Summary | Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222978 | Usp37 (untagged) - Mouse ubiquitin specific peptidase 37 (Usp37), (10ug) |
CNY 12,640.00 |
|
| MR226485 | Usp37 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 37 (Usp37) |
CNY 7,176.00 |
|
| MR226485L3 | Lenti ORF clone of Usp37 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 37 (Usp37) |
CNY 9,030.00 |
|
| MR226485L4 | Lenti ORF clone of Usp37 (mGFP-tagged) - Mouse ubiquitin specific peptidase 37 (Usp37) |
CNY 9,030.00 |
