Srsf4 (NM_020587) Mouse Tagged ORF Clone
CAT#: MG226398
- TrueORF®
Srsf4 (tGFP-tagged) - Mouse serine/arginine-rich splicing factor 4 (Srsf4), (10ug)
ORF Plasmid: DDK
"NM_020587" in other vectors (2)
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CNY 5,420.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5730499P16Rik; AW550192; MNCb-261; MNCb-2616; Sf; Sfrs4; SR; SRp75 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226398 representing NM_020587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGGGTGTACATCGGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGGAGCGCTTCTTTAAGG GCTACGGGAAGATCCTGGAGGTGGATCTGAAGAACGGGTATGGCTTTGTGGAGTTTGATGATCTGCGTGA TGCAGACGATGCTGTTTATGAACTGAACGGCAAAGACCTGTGTGGCGAGCGAGTCATTGTTGAGCACGCT CGAGGCCCGCGGCGGGATGGCAGCTACGGCTCGGGACGCAGTGGATATGGTTATCGAAGAAGTGGCCGAG ATAAATACGGTCCCCCTACTCGCACAGAATACAGACTTATTGTGGAGAATTTGTCAAGTCGATGCAGCTG GCAGGACCTAAAGGATTACATGCGTCAGGCAGGAGAAGTGACGTATGCAGATGCTCACAAGGGACGCAAA AACGAAGGAGTGATTGAATTTGTGTCTTACTCTGATATGAAAAGAGCTTTGGAAAAGCTGGACGGAACTG AAGTCAACGGCAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCTAGGCGGCGCCGGTCTTACTCCAG AAGCCGGAGTCACTCAAGGTCTCGCTCTCGAAGCAGACATTCCCGGAAGAGCAGAAGCCGGAGTGGTAGC AGTAAAAGCAGCCATTCGAAGAGCCGGTCTCGATCCAGGTCAGGTTCCCACTCCCGCAGCAAGAGCCGCA GTCGCAGTCAGAGCCGCAGCCGCAGTAAGAAGGAGAAAAGCCGGAGCCCCAGCAAAGACAACAAGAGCCG CAGTCGCAGCCGCAGCCCTGACAAGAGCCGCAGTAAGAGTAAAGACCACGCCGAGGACAAGCTCCAAAAC AACGACAGCGCCGGCAAAGCCAAGAGCCACAGCCCCAGCCGCCATGACAGTAAAAGTCGGAGCAGGAGCC AGGAGAGGAGAGCAGAGGAGGAGAGGCGAAGGAGCGTGAGCAGGGCTCGCAGCCAGGAGAAGAGCCGCAG CCAGGAGAAGAGCCTTCTCAAGAGCCGCAGCCGCAGCCGGAGCAAGGTGGGCAGCCGGAGCCGCAGCCGC AGCAAGGACAAGCGGAAGGGAAGGAAGAGAAGCAGGGACGAAAGTCGAAGCCGCAGCCGCAGCAAGAGCG AGCGCAGCCGAAAACACAGCAGCAAGCGGGACAGCAAAGTCAGTAGCAGCAGCAGCAAGAAGAAGAAGGA CACCGACCACTCCCGGTCACCGTCCCGCTCGGTGTCAAAGGAGCGCGAACATGCCAAGGCAGAGTCTGGC CAGAGGGAAAGCCGGGCAGAGGGTGAGAGCGAGGCCCCCAATCCAGAGCCCCGGGCCAGGTCAAGATCCA CCTCCAAATCCAAACCCAATGTCCCGGCAGAGTCGCGCTCCAGGTCAAAATCGGCTTCAAAAACACGCTC TCGGTCCAAGTCTCCATCCAGGTCTGCTTCCAGGTCGCCCTCCCGGTCTAGATCCAGGTCCCACTCAAGG TCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226398 representing NM_020587
Red=Cloning site Green=Tags(s) MPRVYIGRLSYQARERDVERFFKGYGKILEVDLKNGYGFVEFDDLRDADDAVYELNGKDLCGERVIVEHA RGPRRDGSYGSGRSGYGYRRSGRDKYGPPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRK NEGVIEFVSYSDMKRALEKLDGTEVNGRKIRLVEDKPGSRRRRSYSRSRSHSRSRSRSRHSRKSRSRSGS SKSSHSKSRSRSRSGSHSRSKSRSRSQSRSRSKKEKSRSPSKDNKSRSRSRSPDKSRSKSKDHAEDKLQN NDSAGKAKSHSPSRHDSKSRSRSQERRAEEERRRSVSRARSQEKSRSQEKSLLKSRSRSRSKVGSRSRSR SKDKRKGRKRSRDESRSRSRSKSERSRKHSSKRDSKVSSSSSKKKKDTDHSRSPSRSVSKEREHAKAESG QRESRAEGESEAPNPEPRARSRSTSKSKPNVPAESRSRSKSASKTRSRSKSPSRSASRSPSRSRSRSHSR S TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020587 |
ORF Size | 1473 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020587.2, NP_065612.2 |
RefSeq Size | 2254 bp |
RefSeq ORF | 1476 bp |
Locus ID | 57317 |
Gene Summary | The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Sep 2010] |
Documents
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SDS |