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Dclk2 (NM_027539) Mouse Tagged ORF Clone
CAT＃: MG226381
- TrueORF®
Dclk2 (tGFP-tagged) - Mouse doublecortin-like kinase 2 (Dclk2) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_027539" in other vectors (5)
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CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330415M09Rik; AU044875; CL2; Clic; Click-II; CLICK2; Dcamk; Dcamkl2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226381 representing NM_027539
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACAAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCAC GGAGAGGGGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTATTTCAAAGGCCTGGTCTTTGCCATCTCCAACG ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCACTGTCTGACAATGTGAACCTGCC CCAGGGCGTCCGCACCATCTACACCATAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTTCGTAAAGTTGATTACACCAAAAACGTTAATCCGA ACTGGTCTGTGAACATCAAGGGCGGAACCACCCGCACCCTGGCCGTGGCCTCTGCGAAGAGTGAGGTGAA AGAAAGCAAAGACTTCATTAAACCCAAGTTAGTGACTGTGATTCGAAGCGGAGTGAAGCCTAGAAAGGCC GTGCGGATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCTTAACAGATATCACCGAAGCCA TTAAACTAGACTCAGGCGTGGTCAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT GTCCTGGATCATAGCGAATGCCGTGTCCTGAAATCGTCCTACTCTCGAGCCTCAGCTGCGAAGTATTCTG GATCCAGAAGCCCAGGGTTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTGAATGGAACTCCCAGTAGCCA GCTTTCCACTCCGAAGTCCACCAAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGATTG AAGCAGATTTCTGCTCAGGGCAGATCTTCTTCCAACGTAAACGGTGGGCCTGAACTTGACCGTTGCCTGA GCCCTGAAGGTGTGAATGGAAACCGGTGCTCCGAGTCGTTCCCCCTTCTGGAGAAATACAGAATAGGGAA GGTCATCGGGGACGGCAACTTCGCGGTAGTTAAGGAGTGCGTGGACAGGTACACTGGAAAAGAGTTTGCA TTAAAGATTATAGACAAAGCCAAATGCTGTGGAAAGGAGCATCTGATTGAGAACGAAGTGTCAATCCTGC GCCGAGTGAAGCACCCCAACATCATCATGTTGGTTGAAGAGATGGAAACAGCAACTGACCTCTTTCTAGT GATGGAACTGGTCAAAGGTGGAGATCTCTTTGATGCGATTACCTCTTCAACCAAGTACACTGAGAGAGAT GGAAGCGCCATGGTGTACAACCTAGCCAATGCCCTCCGGTACCTGCACAGCCTCAGCATCGTCCACAGGG ACATCAAGCCTGAGAATCTGCTGGTGTGCGAATACCCAGATGGAACCAAGTCTTTGAAGCTGGGAGACTT TGGGCTGGCGACGGTGGTTGAAGGCCCGTTGTACACGGTCTGTGGCACGCCAACTTATGTGGCACCAGAG ATCATAGCTGAAACAGGTTATGGCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACATACATACTTC TCTGTGGATTCCCACCATTCCGGAGTGAGAACAATCTCCAGGAAGATCTCTTTGACCAGATCTTGGCTGG AAAGCTGGAATTCCCAGCCCCCTACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATG CTTCAGGTAAACGTTGAAGCTCGCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATG CATCCCAGGAGAACAATATGCAAGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCC CAAACAGAACAGCACCACCACCGGGGTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGATT TTCTGCAGCAAGCTCTGTCAAGACAGCAGCAGACCATCCAGGGAGCAGACCTCGCCAGTCCCTCCCTCAG CCCAGGAGGCCCCTCCCCCACTGGAGTCTCCCAGGCCCCCTGGTCCTCCAGCCACCTCTGGCTGTGATCT GGCAGGGACCTGGCGCCGCCACCGAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226381 representing NM_027539
Red=Cloning site Green=Tags(s) MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK AKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLE GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF VLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGL KQISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFA LKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERD GSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPE IIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQM LQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQI FCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027539 |
| ORF Size | 2268 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027539.5, NP_081815.3 |
| RefSeq Size | 4047 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 70762 |
| UniProt ID | Q6PGN3 |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203532 | Dclk2 (untagged) - Mouse doublecortin-like kinase 2 (Dclk2), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MG210491 | Dclk2 (tGFP-tagged) - Mouse doublecortin and CaM kinase-like 2 (Dcamkl2) |
CNY 8,270.00 |
|
| MR226381 | Dclk2 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 2 (Dclk2), transcript variant 2 |
CNY 7,510.00 |
|
| MR226381L3 | Lenti ORF clone of Dclk2 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 2 (Dclk2), transcript variant 2 |
CNY 9,410.00 |
|
| MR226381L4 | Lenti ORF clone of Dclk2 (mGFP-tagged) - Mouse doublecortin-like kinase 2 (Dclk2), transcript variant 2 |
CNY 9,410.00 |
