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Rnf2 (NM_011277) Mouse Tagged ORF Clone
CAT＃: MG226095
- TrueORF®
Rnf2 (tGFP-tagged) - Mouse ring finger protein 2 (Rnf2), (10ug)
ORF Plasmid: DDK
"NM_011277" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI326319; AI450156; AU019207; dinG; Ring1B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226095 representing NM_011277
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAGGCTGTGCAGACAAATGGAACTCAACCATTAAGCAAAACATGGGAACTCAGTTTGTATGAGT TACAACGAACACCTCAGGAGGCAATAACAGATGGCTTGGAAATTGTGGTTTCACCTAGAAGTCTACACAG TGAATTAATGTGCCCAATTTGTTTGGATATGTTAAAGAACACCATGACTACAAAGGAGTGTTTACATCGG TTTTGCGCGGATTGTATTATCACAGCCCTTAGAAGTGGCAACAAAGAGTGTCCTACCTGTCGAAAAAAAC TGGTTTCTAAAAGATCACTAAGGCCAGACCCGAACTTTGATGCACTCATCAGCAAGATTTATCCCAGTCG TGATGAGTATGAAGCGCATCAGGAAAGGGTCTTAGCAAGGATCAACAAACACAACAATCAGCAGGCTCTC AGCCACAGCATCGAGGAGGGGCTGAAGATACAGGCCATGAACAGATTACAGCGAGGCAAAAAGCAGCAGA TAGAAAATGGTAGTGGAGCAGAAGATAATGGTGACAGCTCCCACTGTAGTAACGCATCCACACACAGCAA CCAGGAAGCGGGCCCGAGTAACAAACGGACCAAAACCTCTGATGACTCTGGGCTTGAACTTGATAACAAC AATGCAGCAGTGGCGATTGATCCAGTCATGGACGGTGCCAGTGAGATTGAGTTAGTCTTCAGGCCCCATC CAACTCTTATGGAAAAGGACGACAGCGCACAGACGAGATACATAAAGACTTCAGGCAATGCCACTGTTGA TCACTTATCCAAGTATCTGGCTGTGAGGTTAGCTTTAGAAGAACTTCGAAGCAAAGGAGAATCAAACCAG ATGAACCTGGATACAGCCAGTGAGAAGCAGTACACCATTTACATAGCCACAGCCAGTGGCCAGTTCACCG TTTTAAATGGCTCCTTTTCTTTGGAATTGGTCAGTGAGAAATACTGGAAAGTGAACAAACCCATGGAACT TTATTATGCACCCACCAAGGAGCACAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226095 representing NM_011277
Red=Cloning site Green=Tags(s) MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHR FCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQAL SHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNN NAAVAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQ MNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011277 |
| ORF Size | 1008 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011277.2, NP_035407.1 |
| RefSeq Size | 3028 bp |
| RefSeq ORF | 1011 bp |
| Locus ID | 19821 |
| UniProt ID | Q9CQJ4 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15525528, PubMed:22325148, PubMed:28596365). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals (PubMed:15525528, PubMed:28596365). May be involved in the initiation of both imprinted and random X inactivation (PubMed:15525528). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:22325148, PubMed:16710298). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:15525528, PubMed:22325148, PubMed:16710298). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:16710298). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (PubMed:15525528, PubMed:16710298). Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers (PubMed:22226355). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209318 | Rnf2 (untagged) - Mouse ring finger protein 2 (Rnf2), (10ug) |
CNY 3,990.00 |
|
| MR226095 | Rnf2 (Myc-DDK-tagged) - Mouse ring finger protein 2 (Rnf2) |
CNY 3,330.00 |
|
| MR226095L3 | Lenti ORF clone of Rnf2 (Myc-DDK-tagged) - Mouse ring finger protein 2 (Rnf2) |
CNY 5,230.00 |
|
| MR226095L4 | Lenti ORF clone of Rnf2 (mGFP-tagged) - Mouse ring finger protein 2 (Rnf2) |
CNY 5,230.00 |
