Keap1 (NM_001110306) Mouse Tagged ORF Clone
CAT#: MG225871
- TrueORF®
Keap1 (tGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001110306" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | INRF2; mKIAA0132 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225871 representing NM_001110306
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCGAACCCAAGCTTAGCGGGGCTCCCCGCAGCAGCCAGTTCCTGCCCCTGTGGTCAAAGTGCC CCGAGGGGGCCGGGGACGCAGTGATGTATGCCTCCACGGAGTGCAAGGCAGAGGTGACGCCCTCGCAGGA CGGTAACCGAACCTTCAGCTACACACTAGAGGATCACACCAAGCAGGCTTTTGGCGTCATGAACGAGCTT CGCCTGAGCCAGCAACTCTGTGACGTGACCCTGCAGGTCAAATATGAGGACATCCCAGCTGCCCAATTCA TGGCTCACAAAGTGGTGCTGGCCTCCTCCAGCCCAGTCTTTAAAGCCATGTTCACCAACGGGCTTCGGGA GCAGGGCATGGAGGTGGTGTCCATCGAAGGCATCCACCCTAAGGTCATGGAAAGGCTTATTGAGTTCGCC TACACGGCCTCCATCTCCGTGGGCGAGAAGTGTGTCCTGCACGTGATGAACGGGGCGGTCATGTACCAGA TTGACAGCGTGGTTCGAGCCTGCAGCGACTTCCTCGTGCAGCAGCTGGACCCCAGCAACGCCATTGGCAT CGCCAACTTCGCGGAGCAGATCGGCTGCACTGAACTGCACCAGCGTGCCCGGGAGTATATCTACATGCAC TTCGGGGAGGTGGCCAAGCAGGAGGAGTTCTTCAACCTGTCACACTGCCAGCTGGCCACGCTCATCAGCC GGGATGATCTGAACGTACGCTGCGAGTCCGAGGTGTTCCACGCGTGCATCGACTGGGTCAAATACGACTG CCCGCAGCGGCGCTTCTACGTGCAGGCACTGCTGCGGGCCGTGCGCTGCCATGCGCTCACGCCGCGCTTC CTGCAGACACAGCTGCAGAAGTGTGAGATCCTGCAGGCCGACGCGCGCTGCAAGGACTACCTGGTGCAGA TATTCCAGGAGCTCACGCTGCACAAGCCCACGCAGGCAGTGCCCTGCCGCGCGCCCAAAGTGGGCCGCCT CATCTACACAGCGGGCGGTTACTTCCGACAGTCGCTCAGCTACCTGGAGGCCTACAACCCGAGCAATGGC TCCTGGCTGCGCCTGGCCGATCTACAGGTGCCGCGCAGTGGGCTGGCAGGCTGCGTGGTGGGTGGGCTGC TATACGCTGTGGGCGGCCGCAACAACTCTCCGGATGGCAACACTGACTCCAGCGCCCTGGACTGCTACAA CCCCATGACCAACCAGTGGTCGCCCTGTGCCTCTATGAGCGTGCCACGCAACCGCATCGGGGTGGGGGTC ATAGATGGCCACATCTACGCAGTCGGGGGTTCCCACGGCTGCATCCACCACAGCAGCGTGGAGAGATATG AGCCAGAGCGGGACGAGTGGCATCTAGTCGCGCCAATGTTGACACGGAGGATTGGCGTGGGCGTGGCAGT GCTCAACCGCTTGCTGTATGCAGTGGGGGGCTTTGACGGGACTAACCGGCTTAACTCCGCAGAATGTTAC TATCCAGAGAGGAATGAGTGGCGGATGATCACACCGATGAATACCATCCGGAGCGGGGCCGGGGTCTGCG TGCTGCACAACTGTATCTATGCAGCAGGGGGCTACGATGGGCAGGACCAGTTGAACAGTGTGGAGCGCTA CGACGTGGAGACAGAGACCTGGACTTTCGTAGCCCCCATGAGGCATCACCGTAGTGCGCTGGGGATTACT GTGCACCAGGGCAAGATCTACGTCCTCGGAGGCTATGATGGCCACACTTTTCTGGACAGTGTGGAATGCT ATGACCCGGACAGTGATACCTGGAGTGAGGTGACCCGCATGACATCTGGCCGCAGCGGGGTGGGTGTGGC CGTCACCATGGAACCCTGTCGGAAGCAAATTGATCAACAAAACTGTACCTGC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225871 representing NM_001110306
Red=Cloning site Green=Tags(s) MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTPSQDGNRTFSYTLEDHTKQAFGVMNEL RLSQQLCDVTLQVKYEDIPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFA YTASISVGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMH FGEVAKQEEFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHALTPRF LQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSNG SWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCASMSVPRNRIGVGV IDGHIYAVGGSHGCIHHSSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECY YPERNEWRMITPMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRHHRSALGIT VHQGKIYVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001110306 |
ORF Size | 1872 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001110306.1, NP_001103776.1 |
RefSeq Size | 3319 bp |
RefSeq ORF | 1875 bp |
Locus ID | 50868 |
UniProt ID | Q9Z2X8 |
Gene Summary | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219821 | Keap1 (untagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 3, (10ug) |
CNY 7,980.00 |
|
MR225871 | Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 3 |
CNY 4,648.00 |
|
MR225871L3 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 3 |
CNY 6,460.00 |
|
MR225871L4 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 3 |
CNY 6,460.00 |