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Pcdh8 (NM_001042726) Mouse Tagged ORF Clone
CAT＃: MG225812
- TrueORF®
Pcdh8 (tGFP-tagged) - Mouse protocadherin 8 (Pcdh8) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001042726" in other vectors (4)
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CNY 9,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700080P15Rik; P; Papc |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225812 representing NM_001042726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCCTGCGAAGCGCTGGGGCAGCCCTTGCCTTTTCCCCTTGCAGCTATTTAGTCTCTGCTGGGTGC TCTCAGTGGCCCAGAGCAAGACAGTCCGATACAGCACGTTCGAAGAGGATGCCCCTGGCACAGTCATCGG AACCCTTGCAGAAGACCTGCACATGAAAGTGTCTGGAGACACAAGCTTCCGCCTGATGAAGCAATTCAAC AGCTCTCTGCTCCGGGTACGCGAGGGCGACGGGCAGCTGACCGTCGGGGATGCGGGCCTGGACCGTGAGC GTCTGTGTGGCCCGTCCCCACAGTGCGTGCTGGCCTTCGATGTTGTCAGCTTCTCGCAGGAACAGTTCCG CCTGGTGCACGTGGAGGTGGAGGTGAGGGATGTAAACGACCACGCGCCCCGCTTTCCCCGAGCCCAGATC CCGGTGGAGGTCTCAGAGAGCGCTCCGGTAGGCACACGCATCCCCCTGGAGGTGCCCGTGGACGAGGACG TGGGTGCCAACGGGCTGCAGAGTGTACGCCTAGCCGAGCCTCACAGCCCCTTCCGCGTGGAGCTGCAGAC GCGCGCGGACGGTGCCCAGTGTGCGGACCTGGTGCTGCTACAGGAGTTGGACCGCGAGAGCCAGGCCTCC TACAGCCTGGAGCTGGTGGCTCAGGATGGCGGGCGACCGCCGCGTTCCGCCACAGCAGCCCTCAGCGTGC GTGTGCTAGATGCCAATGACCACAGCCCGGCCTTCCCACAGGGTGCCGTGGCAGAGGTGGAATTGGCCGA GGACGCACCTGTGGGATCGCTGCTGCTCGACCTGGACGCTGCCGATCCCGACGAAGGTCCCAACGGCGAC GTTGTGTTCACCTTCGGCGCCCGCACCCCTCCCGAAGCCCGCCACCTCTTCCGGCTCGACCCGCGCTCTG GCCGCCTCACCTTGGCTGGGCAGGTGGACTACGAGCGCCAGGACACCTACGAGCTGGACGTGCGGGCTCA AGACCGAGGTCCAGGGCCCCGGACAGCCACCTGCAAGGTTATCGTGCGCATCCGAGACGTCAACGACAAC GCTCCTGAAATTTCTATCACTCCTCTGGCCGCCCCGGGGGCTCCAGCCACCTCGCCCTTCGCCGCCGCCG CCGCTGCCGCCGCACTCGGAGGAGCGGACGCGGCCTCGTCGACTGGGTCCGGGACACAGGAAGCCGGTAT CACCTCGCTGGTGCCAGAGGGGGCGGCGCGCGAGAGTCTAGTGGCGCTGGTCAGCACCTCGGACAGGGAC TCGGGCGCCAACGGGCAGGTGCGCTGCGCCCTCTACGGGCACGAGCACTTTAGGCTGCAGCCGGCCTATG CTGGCAGCTACCTGGTGGTGACCGCTGCATCTCTGGACCGGGAGCGCATCGCGGAGTACAACCTGACGCT GGTGGCCGAAGACCGTGGCACACCCCCTCTTCGCACCGTCAGGCCCTACACCGTGCGAGTGGGGGACGAG AACGACAACGCACCGATCTTCACCAAGCCAGTCTATGAGGTGTCGGTCCGCGAAAACAACCCTCCAGGCG CCTACCTGGCCACAGTGGCAGCCCGCGACCCTGACGTGGGTCGCAACGGTCAAGTCACCTACCGCCTGGT AGAGGCCGAAGTGGGCCGCTCTGGGGAAGCCGTGTCCACTTACGTCTCAGTAGACCCGGCTACCGGAGCC ATCTACGCTCTCCGTAGTTTTGACTATGAGACTCTGCGCCAGCTTGACGTGCGTGTCCAGGCCAGCGACG GCGGTTCCCCTCAGCTTTCCAGCAATGCTCTGGTGCAAGTGCGGGTGCTGGACCAGAATGATCATTCTCC GATCCTTGTGCATCCGGCGCCGGCTAATGGCTCCCTAGAAGTAGCGGTCCCTGGACGTTCCACCAAGGAC ACAGCTGTGGCGCGCATCCAGGCCCGGGATGCTGACGAAGGAGCCAACGGGGAACTGGCCTTTGATCTGC TGCAGCAGGAGCCACGCGAGGCCTTCTCCATAGGCCGCCACACGGGGGAGATCATGCTTACTGGAGACCT CTCGCAGGAGCCTCCTGGCCGCGTGTTCAAGGCCCTGCTGGTCATATCCGACGGCGGCCGTCCCCCTCTC ACCACCACTGCAACCGTCAGTTTCGTGGTAACAGCCGGGGGCGGGTCAGCTGTGCCTGCCAGCTCCGGGA GCCCAGAGCACTCCCGGCCACCCGGCTCTCGCCTCGCGCCGTCGGGGCCTTCGCTGCAGTGGGACACGCC GCTGATCGTCATCATCGTGTTAGCCGGGAGCTGCACGCTGCTGCTTGCAGCCATCATCGCCATCGCCACC ACCTGCAACCGCCGCAAGAAGGAGCCTTACGGTGCCTCTCCAGGCTTCGGGAAGGAGCCTGCTGCGCCCC CTGTTGCAGTCTGGAAGGGTCATTCATTCAACACCATCTCGGGCCGAGAAGCTGAGAAGTTCAGTGGGAA AGACAGCGGCAAAGGAGACAGTGATTTCAATGACAGTGACTCGGACATCAGCGGGGACGCTTTGAAAAAA GACCTCATCAACCACATGCAGAGTGGACTGTGGGCGTGTACTGCTGAGTGCAAGATCCTAGGTCATTCTG ATCGCTGCTGGAGCCCATCCTGTGCCGGACCCAACGTACACCCCCCTCCTCACCCACCAGCCCAGATGTC AACCTTCTGTAAGAGCACGTCCCTGCCTCGGGATCCTCTGCGCAGGGACAATTACTATCAGGCCCAGCTC CCCAAGACAGTAGGGTTGCAGAGCGTCTATGAGAAAGTACTGCATAGAGACTATGACAGGACAGTCACTT TACTCTCCCCTCCCCGTCCAGGAAGGCTCCCAGACCTGCAGGAGATTGGAGTACCCCTCTATGAATCCCC TCCTGGTAGCAGATACGTGTCCCCGAAGAAGGGAATCAATGAAAATGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225812 representing NM_001042726
Red=Cloning site Green=Tags(s) MSPAKRWGSPCLFPLQLFSLCWVLSVAQSKTVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFN SSLLRVREGDGQLTVGDAGLDRERLCGPSPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQI PVEVSESAPVGTRIPLEVPVDEDVGANGLQSVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAS YSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGD VVFTFGARTPPEARHLFRLDPRSGRLTLAGQVDYERQDTYELDVRAQDRGPGPRTATCKVIVRIRDVNDN APEISITPLAAPGAPATSPFAAAAAAAALGGADAASSTGSGTQEAGITSLVPEGAARESLVALVSTSDRD SGANGQVRCALYGHEHFRLQPAYAGSYLVVTAASLDRERIAEYNLTLVAEDRGTPPLRTVRPYTVRVGDE NDNAPIFTKPVYEVSVRENNPPGAYLATVAARDPDVGRNGQVTYRLVEAEVGRSGEAVSTYVSVDPATGA IYALRSFDYETLRQLDVRVQASDGGSPQLSSNALVQVRVLDQNDHSPILVHPAPANGSLEVAVPGRSTKD TAVARIQARDADEGANGELAFDLLQQEPREAFSIGRHTGEIMLTGDLSQEPPGRVFKALLVISDGGRPPL TTTATVSFVVTAGGGSAVPASSGSPEHSRPPGSRLAPSGPSLQWDTPLIVIIVLAGSCTLLLAAIIAIAT TCNRRKKEPYGASPGFGKEPAAPPVAVWKGHSFNTISGREAEKFSGKDSGKGDSDFNDSDSDISGDALKK DLINHMQSGLWACTAECKILGHSDRCWSPSCAGPNVHPPPHPPAQMSTFCKSTSLPRDPLRRDNYYQAQL PKTVGLQSVYEKVLHRDYDRTVTLLSPPRPGRLPDLQEIGVPLYESPPGSRYVSPKKGINENV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042726 |
| ORF Size | 2919 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042726.3, NP_001036191.1 |
| RefSeq Size | 3725 bp |
| RefSeq ORF | 2922 bp |
| Locus ID | 18530 |
| UniProt ID | Q7TSK3 |
| Gene Summary | This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a type I transmembrane protein composed of an extracellular domain including 6 cadherin ectodomains, a single-pass transmembrane domain and a cytoplasmic tail. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons with the first large exon encoding the extracellular and transmembrane region. Although this gene product is capable of homophilic interaction, it appears to affect cell-cell adhesion indirectly by initiating signaling events that regulate classical cadherin-mediated adhesion. Based on studies on this protein and its orthologs, this protocadherin mainly functions in developing embryos and the central nervous system, but can also function as a tumor suppressor. Alternative splicing yielding isoforms with unique cytoplasmic tails has been reported. [provided by RefSeq, Sep 2009] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222897 | Pcdh8 (untagged) - Mouse protocadherin 8 (Pcdh8), transcript variant 2, (10ug) |
CNY 12,540.00 |
|
| MR225812 | Pcdh8 (Myc-DDK-tagged) - Mouse protocadherin 8 (Pcdh8), transcript variant 2 |
CNY 7,128.00 |
|
| MR225812L3 | Lenti ORF clone of Pcdh8 (Myc-DDK-tagged) - Mouse protocadherin 8 (Pcdh8), transcript variant 2 |
CNY 10,450.00 |
|
| MR225812L4 | Lenti ORF clone of Pcdh8 (mGFP-tagged) - Mouse protocadherin 8 (Pcdh8), transcript variant 2 |
CNY 10,450.00 |
