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Dock1 (NM_001033420) Mouse Tagged ORF Clone
CAT＃: MG225743
- TrueORF®
Dock1 (tGFP-tagged) - Mouse dedicator of cytokinesis 1 (Dock1), (10ug)
ORF Plasmid: DDK
"NM_001033420" in other vectors (2)
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CNY 15,580.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9130006G06Rik; AI854900; D630004B07Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225743 representing NM_001033420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCTGGGTCCCCACCAAGCGAGAGGAGAAGTACGGCGTGGCCTTTTACAACTACGATGCCAGGG GAGCAGATGAACTGTCCCTTCAAATTGGAGACACCGTGCACATCCTAGAAACTTATGAAGGGTGGTACAG AGGCTACACCTTAAGGAAAAAGTCTAAAAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCC ATTGTTGAAGGAAAAGGGCAACATGAAACCGTCATCCCTGGGGACCTCCCCCTTATACAGGAAGTCACCA CAACGCTTCGAGAGTGGTCTACCATCTGGAGGCAACTTTATGTGCAAGATAATAGAGAGATGTTTCGAAG TGTGCGACACATGATCTATGACCTTATTGAATGGCGGTCGCAGATCCTTTCTGGAACTCTGCCCCAGGAT GAGCTCAAAGAGCTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTTGATTTGGACC TGGTGGTGAGGGATGAAGATGGGAACATTTTGGACCCGGAACTAACTAGCACCATCAGTCTCTTCCGAGC ACACGAAGTTGCTTCTAAACAAGTGGAGGAGAGACTGCAAGAGGAGAAATCTCAAAAGCAGAACATGGAT ATCAACAGACAAGCCAAGTTTGCAGCCACCCCATCGCTGGCCTTGTTTGTTAACCTCAAAAATGTGGTTT GTAAAATAGGAGAAGATGCTGAAGTTCTCATGTCTCTTTATGACCCCATGGAGTCCAAATTCATCAGTGA GAACTATCTGGTCCGCTGGTCAAGTTCAGGATTACCAAAAGATATTGATCGGTTACATAACTTGCGAGCT GTGTTTACGGATCTGGGGAGCAAAGACTTGAAAAGGGAGAAAATTAGTTTCGTCTGCCAGATTGTCCGTG TCGGTCGCATGGAGCTGAGGGACAGCAACACGAGGAAGCTGACTTCGGGGCTGCGGCGACCTTTCGGCGT GGCTGTGATGGATGTGACAGACATAATAAACGGGAAAGTCGATGATGAAGATAAACAGCATTTCATCCCC TTCCAGGCGGTGGCAGGGGAGAATGACTTCCTTCAAACTGTCATAAACAAAGTCATTGCAGCCAAAGAAG TCAACCACAAGGGACAAGGTTTGTGGGTAACCTTGAAATTACTTCCTGGAGACATCCATCAGATTCGGAA GGAGTTTCCTCACTTGGTGGACAGGACCACAGCTGTGGCCCGCAAGACAGGGTTTCCTGAGATTATTATG CCAGGTGATGTTCGAAATGATATCTATGTGACATTAGTTCAAGGGGATTTTGATAAAGGCAGTAAAACAA CTGCGAAGAACGTGGAGGTGACAGTGTCTGTCTATGATGAAGATGGGAAGCGATTGGAGCACGTGATTTT CCCCGGTGCTGGCGACGAGGCGATCTCCGAGTACAAATCGGTGATTTATTATCAAGTGAAGCAGCCTCGC TGGTTTGAGACCCTTAAGGTGGCCATCCCCATTGAGGACGTGAACCGGAGTCACCTTCGATTCACCTTCC GCCATAGATCCTCGCAGGACTCCAAGGATAAATCTGAAAAGATCTTCGCACTGGCCTTTGTGAAGCTGAT GAGATACGATGGGACCACCCTGAGAGACGGGGAGCATGACCTTATTGTCTACAAGGCTGAAGCCAAAAAG CTCGAAGATGCAGCCACATACTTGAGCCTCCCTAGTACCAAGGGGGAGTTGGAAGAAAAGGGCCACTCAG CCACAGGCAAAGGAATGCAGAGTCTTGGGAGTTGTACCATCAGCAAAGACTCTTTCCAGATCTCCACTCT GGTGTGTTCCACGAAACTGACCCAGAACGTGGACCTCCTGGGGCTGTTGAAGTGGAGATCCAACACCAAC CTTCTGCAGCAGAACTTGAGGCAGTTGATGAAGGTGGACGGTGGTGAAGTGGTGAAGTTCCTTCAGGACA CCTTGGATGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACGTTTGATACCTTGGTGTTTGA TGCTCTGGTCTTTATTATTGGACTAATCGCGGATAGGAAATTTCAGCATTTTAATCCTGTTTTGGAGACT TACATTAAGAAACACTTCAGTGCAACATTAGCCTACACGAAGCTGACCAAGGTGTTACGGACCTACGTGG CCAGCGCAGAGAAGCCGGGAGTCAACGAGCAGCTGTACAAAGCCATAAAAGCCTTGGAGTACATCTTCAA GTTCATAGTGCGCTCACGGGTACTGTTCAACCAGCTGTATGAAAACAAGGGGGAAGCTGACTTCGTGGAG TCCTTGTTGCAGCTGTTCCGGTCCATCAACGACATGATGAGCAGCCTGTCAGAGCTCACAGTCAGGGTGA AGGGGGCAGCCCTGAAGTACTTGCCGACGATCGTCAATGATGTGAAGTTGGTGTTTGATCCCAAGGAGCT CAGCAAAATGTTCACGGAATTCATCCTGAATGTCCCTGCGGGCTTGCTGACGGTCCAGAAGCTCTCCTGC CTGATTGAGATTGTTCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTCCCCATGATGA CAGACCAGCTCAAGTACCACCTGGAGAGGCAGGAGGAGCTGGAGGCTTGTTGCCAGCTGCTTAGCAACAT CCTGGAGGTGCTATACCGGAAGGATGTGGGACCGACTCAGAGGCACGTCCAGATCATCATGGAGACGCTG CTTAGGACTGTGAACAGGACGGTCATTTCCATGGGGCGGGACTCCGAGCTCATTGGGAACTTTGTGGCTT GCATGACAGCCATTTTACGGCAGATGGAAGACTACCATTATGCACACCTGATCAAGACCTTTGGGAAAAT GAGGACAGATGTCGTGGATTTTCTGATGGAGACATTCATCATGTTTAAGAACCTCATCGGGAAGAACGTC TACCCCTTCGACTGGGTGATAATGAACACGATGCAGAACAAAGTCTTTCTGCGGGCGATTAATCAGTATG CAGATATGCTGAACAAGAGATTTCTGGATCAAGCCAACTTTGAGCTGCAGCTGTGGAACAACTACTTTCA CCTGGCCGTGGCTTTCCTCACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCTAAGAGAGCTAAA ATCCTTAACAAGTATGGAGACATGAGGAGGCAGATTGGCTTTGAGATCAGAGACATGTGGTACAACCTGG GTCAACACAAGATAAAATTCATCCCAGAAATGGTTGGCCCAATATTGGAAATGACATTGATTCCTGAGAC AGAGCTTCGAAAAGCCACCCTTCCCATCTTCTTCGACATGATGCAGTGTGAATTCCACTCCACCCGGAGC TTTCAAATGTTTGAAAATGAGATCATCACTAAGCTGGATCATGAAGTGGAAGGAGGCAGAGGAGACGAGC AATACAAAGTGTTATTTGATAAAATCCTCCTGGAGCACTGCAGGAAGCATAAATACCTCGCCAAGACAGG AGAAACTTTTGTGAAGCTCGTAGTGCGGTTGATGGAGAGGCTCCTGGACTACAGGACCATCATGCATGAC GAAAACAAAGACAATCGGATGAGCTGCACCGTCAACGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAG AGATGTATATAAGGTACTTGTATAAGCTCTGTGACCTGCACAAAGAGTGTGACAACTACACAGAGGCGGC CTACACTTTGCTTCTGCACGCGAAACTTCTAAAGTGGTCAGAGGACGTGTGTGCAGCTCACCTCACTCAG CGGGACGGGTTCCAGGCGACAACACAGGGACAGCTGAAAGAGCAGCTCTACCAAGAAATCATCCACTACT TTGACAAAGGCAAGATGTGGGAGGAGGCCATCGCCCTGGGCAAGGAACTTGCGGAGCAGTACGAAACAGA GATGTTTGATTATGAGCAGCTCAGTGAATTGCTGAAAAAACAGGCACAATTTTACGAGAACATCGTGAAG GTGATAAGACCCAAGCCTGACTATTTTGCTGTTGGCTACTATGGGCAAGGGTTCCCGTCATTCCTTCGGG GCAAAGTGTTCATTTACCGAGGCAAGGAATATGAGCGACGGGAAGATTTTGAAGCTCGGCTGTTAACTCA GTTTCCAAATGCTGAAAAAATGAAGACGACGTCTCCACCGGGCGATGACATTAAGACTTCTCCCGGCCAG TACATCCAGTGCTTCACAGTGAAGCCCAAGCTAGACTTACCTCCGAGATTTCACCGACCCGTGTCTGAAC AGATTGTAAGTTTTTACAGGGTGAATGAGGTACAGCGATTCGAGTATTCACGGCCCATCCGGAAAGGAGA GAAAAACCCAGACAATGAATTTGCGAATATGTGGATTGAAAGAACCATCTACACTACGGCCTATAAACTG CCAGGAATTTTAAGGTGGTTTGAAGTCAAATCTGTCTTCATGGTAGAGATCAGCCCACTGGAGAATGCCA TCGAGACAATGCAGTTGACCAATGACAAGATCAGCAGCATGGTCCAGCAGCACCTGGATGACCCGGGCCT GCCTATCAACCCCCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGTGGTTTTGCCAAT TATGAGAAGGCTTTCTTCACAGACAGATACCTGCAGGAGCATCCTGAGGCCCATGGGCAGATTGAGAAGC TCAAGGACCTGATAGCCTGGCAGATTCCATTTTTGGCTGAAGGGATCAGGATTCATGGAGACAAAGTCAC AGAGGCCCTGAGGCCATTCCACGAGCGCATGGAAGCCTGCTTCAAGCAACTGAAGGAAAAAGTGGAAAAG CAATATGGCGTCAGGACCATGCCCTCAGGCTTGGATGACAGGAGAGGCAGCCGTCCACGTTCAATGGTGC GATCTTTCACCATGCCATCATCCTCCCGGCCCCTGTCAGTGGCTTCCGTCTCTTCTTTCTCCTCTGACAG CACTCCTTCCAGGCCAGGCTCTGATGGATTTGCTCTAGAGCCTCTGCTGCCGAAGAAGATGCACTCCAGG TCCCAGGACAAGCTAGACAAGGACGATCCAGATAAAGAGAAGAAGGACAAGAAGAAGGAGAAAAGGAACA GCAAACACCAAGAGATATTTGATAAGGAATTCAAGCCCGCTGACAGCTCCCTGCAGCAGTCTGAGGCTGT GATCCTTTCTGAAACGATAAGTCCCCTGCGGCCCCAAAGACCCAAGAGCCAGGTGATCAATGTCATTGGA AATGAAAGGCGCTTCTCTGTGTCTCCAGCATCACCGTCCTCCCAGCAGACGCCCCCTCCAGTCACACCAA GAGCCAAGCTCAGCTTCAGCATTCAGCCCAGTCTGGAGCTGAACGGCATGATGGGAATGGATGTGGCCGA TGTCCCACCCCCTCTGCCCCTCAAAGGCAACATGGCAGATTATGGAAATCTGATGGAAAACCAGGATATG ATGGTCTCACCCACATCTCCACCTCCTCCTCCCCCACAACGGCAACAGCCACCTCCACTGCCCAGCAAAA CACCACCTCCTCCCCCTCCGAAGACGACCCGAAAGCAGACCTCAGTGGACTCTGGAATCGTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225743 representing NM_001033420
Red=Cloning site Green=Tags(s) MTRWVPTKREEKYGVAFYNYDARGADELSLQIGDTVHILETYEGWYRGYTLRKKSKKGIFPASYIHLKEA IVEGKGQHETVIPGDLPLIQEVTTTLREWSTIWRQLYVQDNREMFRSVRHMIYDLIEWRSQILSGTLPQD ELKELKKKVTAKIDYGNRILDLDLVVRDEDGNILDPELTSTISLFRAHEVASKQVEERLQEEKSQKQNMD INRQAKFAATPSLALFVNLKNVVCKIGEDAEVLMSLYDPMESKFISENYLVRWSSSGLPKDIDRLHNLRA VFTDLGSKDLKREKISFVCQIVRVGRMELRDSNTRKLTSGLRRPFGVAVMDVTDIINGKVDDEDKQHFIP FQAVAGENDFLQTVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKTGFPEIIM PGDVRNDIYVTLVQGDFDKGSKTTAKNVEVTVSVYDEDGKRLEHVIFPGAGDEAISEYKSVIYYQVKQPR WFETLKVAIPIEDVNRSHLRFTFRHRSSQDSKDKSEKIFALAFVKLMRYDGTTLRDGEHDLIVYKAEAKK LEDAATYLSLPSTKGELEEKGHSATGKGMQSLGSCTISKDSFQISTLVCSTKLTQNVDLLGLLKWRSNTN LLQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSESETFDTLVFDALVFIIGLIADRKFQHFNPVLET YIKKHFSATLAYTKLTKVLRTYVASAEKPGVNEQLYKAIKALEYIFKFIVRSRVLFNQLYENKGEADFVE SLLQLFRSINDMMSSLSELTVRVKGAALKYLPTIVNDVKLVFDPKELSKMFTEFILNVPAGLLTVQKLSC LIEIVHSDLFTQHDCREILLPMMTDQLKYHLERQEELEACCQLLSNILEVLYRKDVGPTQRHVQIIMETL LRTVNRTVISMGRDSELIGNFVACMTAILRQMEDYHYAHLIKTFGKMRTDVVDFLMETFIMFKNLIGKNV YPFDWVIMNTMQNKVFLRAINQYADMLNKRFLDQANFELQLWNNYFHLAVAFLTQESLQLENFSSAKRAK ILNKYGDMRRQIGFEIRDMWYNLGQHKIKFIPEMVGPILEMTLIPETELRKATLPIFFDMMQCEFHSTRS FQMFENEIITKLDHEVEGGRGDEQYKVLFDKILLEHCRKHKYLAKTGETFVKLVVRLMERLLDYRTIMHD ENKDNRMSCTVNVLNFYKEIEREEMYIRYLYKLCDLHKECDNYTEAAYTLLLHAKLLKWSEDVCAAHLTQ RDGFQATTQGQLKEQLYQEIIHYFDKGKMWEEAIALGKELAEQYETEMFDYEQLSELLKKQAQFYENIVK VIRPKPDYFAVGYYGQGFPSFLRGKVFIYRGKEYERREDFEARLLTQFPNAEKMKTTSPPGDDIKTSPGQ YIQCFTVKPKLDLPPRFHRPVSEQIVSFYRVNEVQRFEYSRPIRKGEKNPDNEFANMWIERTIYTTAYKL PGILRWFEVKSVFMVEISPLENAIETMQLTNDKISSMVQQHLDDPGLPINPLSMLLNGIVDPAVMGGFAN YEKAFFTDRYLQEHPEAHGQIEKLKDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEK QYGVRTMPSGLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSFSSDSTPSRPGSDGFALEPLLPKKMHSR SQDKLDKDDPDKEKKDKKKEKRNSKHQEIFDKEFKPADSSLQQSEAVILSETISPLRPQRPKSQVINVIG NERRFSVSPASPSSQQTPPPVTPRAKLSFSIQPSLELNGMMGMDVADVPPPLPLKGNMADYGNLMENQDM MVSPTSPPPPPPQRQQPPPLPSKTPPPPPPKTTRKQTSVDSGIVQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033420 |
| ORF Size | 5595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033420.2, NP_001028592.1 |
| RefSeq Size | 6815 bp |
| RefSeq ORF | 5598 bp |
| Locus ID | 330662 |
| UniProt ID | Q8BUR4 |
| Gene Summary | Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.[UniProtKB/Swiss-Prot Function] |
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