Dock1 (NM_001033420) Mouse Untagged Clone

CAT#: MC224993

Dock1 (untagged) - Mouse dedicator of cytokinesis 1 (Dock1), (10ug)



  "NM_001033420" in other vectors (2)

CNY 29,930.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9130006G06Rik; AI854900; D630004B07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224993 representing NM_001033420
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCGCTGGGTCCCCACCAAGCGAGAGGAGAAGTACGGCGTGGCCTTTTACAACTACGATGCCAGGG
GAGCAGATGAACTGTCCCTTCAAATTGGAGACACCGTGCACATCCTAGAAACTTATGAAGGGTGGTACAG
AGGCTACACCTTAAGGAAAAAGTCTAAAAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCC
ATTGTTGAAGGAAAAGGGCAACATGAAACCGTCATCCCTGGGGACCTCCCCCTTATACAGGAAGTCACCA
CAACGCTTCGAGAGTGGTCTACCATCTGGAGGCAACTTTATGTGCAAGATAATAGAGAGATGTTTCGAAG
TGTGCGACACATGATCTATGACCTTATTGAATGGCGGTCGCAGATCCTTTCTGGAACTCTGCCCCAGGAT
GAGCTCAAAGAGCTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTTGATTTGGACC
TGGTGGTGAGGGATGAAGATGGGAACATTTTGGACCCGGAACTAACTAGCACCATCAGTCTCTTCCGAGC
ACACGAAGTTGCTTCTAAACAAGTGGAGGAGAGACTGCAAGAGGAGAAATCTCAAAAGCAGAACATGGAT
ATCAACAGACAAGCCAAGTTTGCAGCCACCCCATCGCTGGCCTTGTTTGTTAACCTCAAAAATGTGGTTT
GTAAAATAGGAGAAGATGCTGAAGTTCTCATGTCTCTTTATGACCCCATGGAGTCCAAATTCATCAGTGA
GAACTATCTGGTCCGCTGGTCAAGTTCAGGATTACCAAAAGATATTGATCGGTTACATAACTTGCGAGCT
GTGTTTACGGATCTGGGGAGCAAAGACTTGAAAAGGGAGAAAATTAGTTTCGTCTGCCAGATTGTCCGTG
TCGGTCGCATGGAGCTGAGGGACAGCAACACGAGGAAGCTGACTTCGGGGCTGCGGCGACCTTTCGGCGT
GGCTGTGATGGATGTGACAGACATAATAAACGGGAAAGTCGATGATGAAGATAAACAGCATTTCATCCCC
TTCCAGGCGGTGGCAGGGGAGAATGACTTCCTTCAAACTGTCATAAACAAAGTCATTGCAGCCAAAGAAG
TCAACCACAAGGGACAAGGTTTGTGGGTAACCTTGAAATTACTTCCTGGAGACATCCATCAGATTCGGAA
GGAGTTTCCTCACTTGGTGGACAGGACCACAGCTGTGGCCCGCAAGACAGGGTTTCCTGAGATTATTATG
CCAGGTGATGTTCGAAATGATATCTATGTGACATTAGTTCAAGGGGATTTTGATAAAGGCAGTAAAACAA
CTGCGAAGAACGTGGAGGTGACAGTGTCTGTCTATGATGAAGATGGGAAGCGATTGGAGCACGTGATTTT
CCCCGGTGCTGGCGACGAGGCGATCTCCGAGTACAAATCGGTGATTTATTATCAAGTGAAGCAGCCTCGC
TGGTTTGAGACCCTTAAGGTGGCCATCCCCATTGAGGACGTGAACCGGAGTCACCTTCGATTCACCTTCC
GCCATAGATCCTCGCAGGACTCCAAGGATAAATCTGAAAAGATCTTCGCACTGGCCTTTGTGAAGCTGAT
GAGATACGATGGGACCACCCTGAGAGACGGGGAGCATGACCTTATTGTCTACAAGGCTGAAGCCAAAAAG
CTCGAAGATGCAGCCACATACTTGAGCCTCCCTAGTACCAAGGGGGAGTTGGAAGAAAAGGGCCACTCAG
CCACAGGCAAAGGAATGCAGAGTCTTGGGAGTTGTACCATCAGCAAAGACTCTTTCCAGATCTCCACTCT
GGTGTGTTCCACGAAACTGACCCAGAACGTGGACCTCCTGGGGCTGTTGAAGTGGAGATCCAACACCAAC
CTTCTGCAGCAGAACTTGAGGCAGTTGATGAAGGTGGACGGTGGTGAAGTGGTGAAGTTCCTTCAGGACA
CCTTGGATGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACGTTTGATACCTTGGTGTTTGA
TGCTCTGGTCTTTATTATTGGACTAATCGCGGATAGGAAATTTCAGCATTTTAATCCTGTTTTGGAGACT
TACATTAAGAAACACTTCAGTGCAACATTAGCCTACACGAAGCTGACCAAGGTGTTACGGACCTACGTGG
CCAGCGCAGAGAAGCCGGGAGTCAACGAGCAGCTGTACAAAGCCATAAAAGCCTTGGAGTACATCTTCAA
GTTCATAGTGCGCTCACGGGTACTGTTCAACCAGCTGTATGAAAACAAGGGGGAAGCTGACTTCGTGGAG
TCCTTGTTGCAGCTGTTCCGGTCCATCAACGACATGATGAGCAGCCTGTCAGAGCTCACAGTCAGGGTGA
AGGGGGCAGCCCTGAAGTACTTGCCGACGATCGTCAATGATGTGAAGTTGGTGTTTGATCCCAAGGAGCT
CAGCAAAATGTTCACGGAATTCATCCTGAATGTCCCTGCGGGCTTGCTGACGGTCCAGAAGCTCTCCTGC
CTGATTGAGATTGTTCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTCCCCATGATGA
CAGACCAGCTCAAGTACCACCTGGAGAGGCAGGAGGAGCTGGAGGCTTGTTGCCAGCTGCTTAGCAACAT
CCTGGAGGTGCTATACCGGAAGGATGTGGGACCGACTCAGAGGCACGTCCAGATCATCATGGAGACGCTG
CTTAGGACTGTGAACAGGACGGTCATTTCCATGGGGCGGGACTCCGAGCTCATTGGGAACTTTGTGGCTT
GCATGACAGCCATTTTACGGCAGATGGAAGACTACCATTATGCACACCTGATCAAGACCTTTGGGAAAAT
GAGGACAGATGTCGTGGATTTTCTGATGGAGACATTCATCATGTTTAAGAACCTCATCGGGAAGAACGTC
TACCCCTTCGACTGGGTGATAATGAACACGATGCAGAACAAAGTCTTTCTGCGGGCGATTAATCAGTATG
CAGATATGCTGAACAAGAGATTTCTGGATCAAGCCAACTTTGAGCTGCAGCTGTGGAACAACTACTTTCA
CCTGGCCGTGGCTTTCCTCACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCTAAGAGAGCTAAA
ATCCTTAACAAGTATGGAGACATGAGGAGGCAGATTGGCTTTGAGATCAGAGACATGTGGTACAACCTGG
GTCAACACAAGATAAAATTCATCCCAGAAATGGTTGGCCCAATATTGGAAATGACATTGATTCCTGAGAC
AGAGCTTCGAAAAGCCACCCTTCCCATCTTCTTCGACATGATGCAGTGTGAATTCCACTCCACCCGGAGC
TTTCAAATGTTTGAAAATGAGATCATCACTAAGCTGGATCATGAAGTGGAAGGAGGCAGAGGAGACGAGC
AATACAAAGTGTTATTTGATAAAATCCTCCTGGAGCACTGCAGGAAGCATAAATACCTCGCCAAGACAGG
AGAAACTTTTGTGAAGCTCGTAGTGCGGTTGATGGAGAGGCTCCTGGACTACAGGACCATCATGCATGAC
GAAAACAAAGACAATCGGATGAGCTGCACCGTCAACGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAG
AGATGTATATAAGGTACTTGTATAAGCTCTGTGACCTGCACAAAGAGTGTGACAACTACACAGAGGCGGC
CTACACTTTGCTTCTGCACGCGAAACTTCTAAAGTGGTCAGAGGACGTGTGTGCAGCTCACCTCACTCAG
CGGGACGGGTTCCAGGCGACAACACAGGGACAGCTGAAAGAGCAGCTCTACCAAGAAATCATCCACTACT
TTGACAAAGGCAAGATGTGGGAGGAGGCCATCGCCCTGGGCAAGGAACTTGCGGAGCAGTACGAAACAGA
GATGTTTGATTATGAGCAGCTCAGTGAATTGCTGAAAAAACAGGCACAATTTTACGAGAACATCGTGAAG
GTGATAAGACCCAAGCCTGACTATTTTGCTGTTGGCTACTATGGGCAAGGGTTCCCGTCATTCCTTCGGG
GCAAAGTGTTCATTTACCGAGGCAAGGAATATGAGCGACGGGAAGATTTTGAAGCTCGGCTGTTAACTCA
GTTTCCAAATGCTGAAAAAATGAAGACGACGTCTCCACCGGGCGATGACATTAAGACTTCTCCCGGCCAG
TACATCCAGTGCTTCACAGTGAAGCCCAAGCTAGACTTACCTCCGAGATTTCACCGACCCGTGTCTGAAC
AGATTGTAAGTTTTTACAGGGTGAATGAGGTACAGCGATTCGAGTATTCACGGCCCATCCGGAAAGGAGA
GAAAAACCCAGACAATGAATTTGCGAATATGTGGATTGAAAGAACCATCTACACTACGGCCTATAAACTG
CCAGGAATTTTAAGGTGGTTTGAAGTCAAATCTGTCTTCATGGTAGAGATCAGCCCACTGGAGAATGCCA
TCGAGACAATGCAGTTGACCAATGACAAGATCAGCAGCATGGTCCAGCAGCACCTGGATGACCCGGGCCT
GCCTATCAACCCCCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGTGGTTTTGCCAAT
TATGAGAAGGCTTTCTTCACAGACAGATACCTGCAGGAGCATCCTGAGGCCCATGGGCAGATTGAGAAGC
TCAAGGACCTGATAGCCTGGCAGATTCCATTTTTGGCTGAAGGGATCAGGATTCATGGAGACAAAGTCAC
AGAGGCCCTGAGGCCATTCCACGAGCGCATGGAAGCCTGCTTCAAGCAACTGAAGGAAAAAGTGGAAAAG
CAATATGGCGTCAGGACCATGCCCTCAGGCTTGGATGACAGGAGAGGCAGCCGTCCACGTTCAATGGTGC
GATCTTTCACCATGCCATCATCCTCCCGGCCCCTGTCAGTGGCTTCCGTCTCTTCTTTCTCCTCTGACAG
CACTCCTTCCAGGCCAGGCTCTGATGGATTTGCTCTAGAGCCTCTGCTGCCGAAGAAGATGCACTCCAGG
TCCCAGGACAAGCTAGACAAGGACGATCCAGATAAAGAGAAGAAGGACAAGAAGAAGGAGAAAAGGAACA
GCAAACACCAAGAGATATTTGATAAGGAATTCAAGCCCGCTGACAGCTCCCTGCAGCAGTCTGAGGCTGT
GATCCTTTCTGAAACGATAAGTCCCCTGCGGCCCCAAAGACCCAAGAGCCAGGTGATCAATGTCATTGGA
AATGAAAGGCGCTTCTCTGTGTCTCCAGCATCACCGTCCTCCCAGCAGACGCCCCCTCCAGTCACACCAA
GAGCCAAGCTCAGCTTCAGCATTCAGCCCAGTCTGGAGCTGAACGGCATGATGGGAATGGATGTGGCCGA
TGTCCCACCCCCTCTGCCCCTCAAAGGCAACATGGCAGATTATGGAAATCTGATGGAAAACCAGGATATG
ATGGTCTCACCCACATCTCCACCTCCTCCTCCCCCACAACGGCAACAGCCACCTCCACTGCCCAGCAAAA
CACCACCTCCTCCCCCTCCGAAGACGACCCGAAAGCAGACCTCAGTGGACTCTGGAATCGTGCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001033420
Insert Size 5598 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001033420.2, NP_001028592.1
RefSeq Size 6815 bp
RefSeq ORF 5598 bp
Locus ID 330662
UniProt ID Q8BUR4
Gene Summary Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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