Lamp1 (NM_010684) Mouse Tagged ORF Clone
CAT#: MG225631
- TrueORF®
Lamp1 (tGFP-tagged) - Mouse lysosomal-associated membrane protein 1 (Lamp1), (10ug)
ORF Plasmid: DDK
"NM_010684" in other vectors (4)
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CNY 5,256.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CD107a; Lamp-1; LGP-120; LGP-A; P2B; Perk |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225631 representing NM_010684
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCCCGGCGCCCGGCGGCCGCTGCTCCTGCTGCTGCTGGCAGGCCTTGCACATGGCGCCTCAG CACTCTTTGAGGTGAAAAACAATGGCACAACGTGTATAATGGCCAGCTTCTCTGCCTCCTTTCTGACCAC CTACGAGACTGCGAATGGTTCTCAGATCGTGAACATTTCCCTGCCAGCCTCTGCAGAAGTACTGAAAAAT GGCAGTTCTTGTGGTAAAGAAAATGTTTCTGACCCCAGCCTCACAATTACTTTTGGAAGAGGATATTTAC TGACACTCAACTTCACAAAAAATACAACACGTTACAGTGTCCAGCATATGTATTTTACATATAACTTGTC AGATACAGAACATTTTCCCAATGCCATCAGCAAAGAGATCTACACCATGGATTCCACAACTGACATCAAG GCAGACATCAACAAAGCATACCGGTGTGTCAGTGATATCCGGGTCTACATGAAGAATGTGACCGTTGTGC TCCGGGATGCCACTATCCAGGCCTACCTGTCGAGTGGCAACTTCAGCAAGGAAGAGACACACTGCACACA GGATGGACCTTCCCCAACCACTGGGCCACCCAGCCCCTCACCACCACTTGTGCCCACAAACCCCACTGTA TCCAAGTACAATGTTACTGGTAACAACGGAACCTGCCTGCTGGCCTCTATGGCACTGCAACTGAATATCA CCTACCTGAAAAAGGACAACAAGACGGTGACCAGAGCGTTCAACATCAGCCCAAATGACACATCTAGTGG GAGTTGCGGTATCAACTTGGTGACCCTGAAAGTGGAGAACAAGAACAGAGCCCTGGAATTGCAGTTTGGG ATGAATGCCAGCTCTAGCCTGTTTTTCCTGCAAGGAGTGCGCTTGAATATGACTCTTCCTGATGCCCTAG TGCCCACATTCAGCATCTCCAACCATTCACTGAAAGCTCTTCAGGCCACTGTGGGAAACTCATACAAGTG CAACACTGAGGAACACATCTTTGTCAGCAAGATGCTCTCCCTCAATGTCTTCAGTGTGCAGGTCCAGGCT TTCAAGGTGGACAGTGACAGGTTTGGGTCTGTGGAAGAGTGTGTTCAGGATGGTAACAACATGTTGATCC CCATTGCTGTGGGCGGTGCCCTGGCAGGGCTGGTCCTCATCGTCCTCATTGCCTACCTCATTGGCAGGAA GAGGAGTCACGCCGGCTATCAGACCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225631 representing NM_010684
Red=Cloning site Green=Tags(s) MAAPGARRPLLLLLLAGLAHGASALFEVKNNGTTCIMASFSASFLTTYETANGSQIVNISLPASAEVLKN GSSCGKENVSDPSLTITFGRGYLLTLNFTKNTTRYSVQHMYFTYNLSDTEHFPNAISKEIYTMDSTTDIK ADINKAYRCVSDIRVYMKNVTVVLRDATIQAYLSSGNFSKEETHCTQDGPSPTTGPPSPSPPLVPTNPTV SKYNVTGNNGTCLLASMALQLNITYLKKDNKTVTRAFNISPNDTSSGSCGINLVTLKVENKNRALELQFG MNASSSLFFLQGVRLNMTLPDALVPTFSISNHSLKALQATVGNSYKCNTEEHIFVSKMLSLNVFSVQVQA FKVDSDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKRSHAGYQTI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010684 |
ORF Size | 1218 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010684.3 |
RefSeq Size | 2265 bp |
RefSeq ORF | 1221 bp |
Locus ID | 16783 |
UniProt ID | P11438 |
Gene Summary | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Direct homophilic interaction of LAMP2A with the two-domain architecture revealed by site-directed photo-crosslinks and steric hindrances in mammalian cells
,Terasawa, K;Kato, Y;Ikami, Y;Sakamoto, K;Ohtake, K;Kusano, S;Tomabechi, Y;Kukimoto-Niino, M;Shirouzu, M;Guan, JL;Kobayashi, T;Iwata, T;Watabe, T;Yokoyama, S;Hara-Yokoyama, M;,
Autophagy
,PubMed ID 33849387
[LAMP1]
|
Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes
,Terasawa, K;Tomabechi, Y;Ikeda, M;Ehara, H;Kukimoto-Niino, M;Wakiyama, M;Podyma-Inoue, KA;Rajapakshe, AR;Watabe, T;Shirouzu, M;Hara-Yokoyama, M;,
Biochem. Biophys. Res. Commun.
,PubMed ID 27663661
[Lamp1]
|
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208861 | Lamp1 (untagged) - Mouse lysosomal-associated membrane protein 1 (Lamp1), (10ug) |
CNY 3,656.00 |
|
MR225631 | Lamp1 (Myc-DDK-tagged) - Mouse lysosomal-associated membrane protein 1 (Lamp1) |
CNY 3,656.00 |
|
MR225631L3 | Lenti ORF clone of Lamp1 (Myc-DDK-tagged) - Mouse lysosomal-associated membrane protein 1 (Lamp1) |
CNY 4,750.00 |
|
MR225631L4 | Lenti ORF clone of Lamp1 (mGFP-tagged) - Mouse lysosomal-associated membrane protein 1 (Lamp1) |
CNY 6,056.00 |