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Tirap (NM_001177846) Mouse Tagged ORF Clone
CAT＃: MG225273
- TrueORF®
Tirap (tGFP-tagged) - Mouse toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001177846" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA407980; C130027E04Rik; Mal; Tlr4ap; Wyatt |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225273 representing NM_001177846
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCATCCTCCTCCGTCCCAGCCTCCTCCACTCCGTCCAAGAAGCCTCGAGACAAGATAGCTGACT GGTTCAGGCAGGCTCTGTTGAAGAAGCCCAAGAAGATGCCGATCTCCCAGGAAAGCCACCTCTATGATGG TTCACAGACAGCCACACAGGATGGTCTCTCACCCTCGAGCTGCAGCTCACCCCCGAGTCACAGTTCACCC GAGAGCCGTAGCTCACCCTCGAGCTGCAGTTCAGGAATGTCACCTACCTCGCCACCAACACACGTGGACA GCAGCAGCAGCAGCAGTGGCCGCTGGAGCAAAGACTACGATGTCTGCGTGTGCCACAGTGAGGAGGACTT GGAGGCGGCCCAGGAGCTGGTCTCCTACTTGGAGGGTAGCCAGGCCAGTCTACGCTGCTTCCTGCAGCTT CGGGATGCAGCCCCGGGTGGCGCCATTGTTTCGGAGCTATGCCAGGCACTGAGTCGTAGTCACTGCCGTG CGCTGCTCATCACTCCAGGCTTCCTTCGGGACCCCTGGTGCAAGTACCAGATGCTGCAGGCCCTGACGGA GGCCCCGGCGTCGGAGGGTTGCACCATACCCCTGCTGTCCGGCCTGTCCAGAGCCGCCTATCCGCCGGAA CTCCGATTCATGTACTATGTGGATGGCAGAGGCAAGGACGGAGGCTTTTACCAAGTCAAGGAGGCTGTTA TACACTATCTGGAGACACTAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225273 representing NM_001177846
Red=Cloning site Green=Tags(s) MASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSP ESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQL RDAAPGGAIVSELCQALSRSHCRALLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPE LRFMYYVDGRGKDGGFYQVKEAVIHYLETLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177846 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177846.1, NP_001171317.1 |
| RefSeq Size | 4731 bp |
| RefSeq ORF | 750 bp |
| Locus ID | 117149 |
| Gene Summary | Adapter involved in the TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response (By similarity). Positively regulates the production of TNF-alpha and interleukin-6 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212300 | Tirap (untagged) - Mouse toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
| MR225273 | Tirap (Myc-DDK-tagged) - Mouse toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3 |
CNY 2,400.00 |
|
| MR225273L3 | Lenti ORF clone of Tirap (Myc-DDK-tagged) - Mouse toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3 |
CNY 4,750.00 |
|
| MR225273L4 | Lenti ORF clone of Tirap (mGFP-tagged) - Mouse toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3 |
CNY 4,750.00 |
