Dlc1 (NM_001194941) Mouse Tagged ORF Clone
CAT#: MG225230
- TrueORF®
Dlc1 (tGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001194941" in other vectors (4)
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CNY 12,350.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A730069N07Rik; Arhgap7; dlc-1; HP; STARD12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225230 representing NM_001194941
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCCGAGGGCCACGTGATGGCTCGGCCCCTGAGAGGCCCGCTGCGGAGGTCCTTCAGCGACC ACATCAGAGACTCCACCGCCAGGGCGCTGGATGCTATCTGGAAAAACACCAGAGAGAGACGGCTAGCAGA AATTGAAGCCAAGGAAGCTTGCGACTGGCTGAGAGTGACTGGTTTCCCCCAGTATGCACAGCTGTATGAA GATCTACTGTTCCCTATTGATATTGCGCTGGTCAAGAGAGAACATGACTTCTTGGACAGAGACGCCATCG AGGCTCTCTGCAGGCGCTTAAATACTCTAAACAAGTGTGCAGTCATGAAGCTGGAGATCAGTCCTCACCG GAAGCGAAGTGAGGATTCGGATGAAGATGAGCCTTGCGCCATAAGCGGCAAGTGGACTTTCCAGAGGGAC AGCAAGCGGTGGTCCCGCCTTGAAGAGTTTGACGTCTTTTCCCCAAAGCAGGATCCAATCCCTGGGTCCC CAGACAACTCTCGTTTGCAAAGCGCCACAAGCCACGAAAGCATGCTGACAGACCTCAGCGAGCACCAGGA GGTGGCCTCTGTCCGAAGCCTCAGCAGCACCAGCAGCAGCGTCCCCACCCACGCAGCCCACAGTGGAGAT GCCACTACGCCCCGAACCAATTCCGTCATCAGCGTCTGCTCCTCCGGACACTTTGTAGGCAATGATGACT CTTTTTCCAGCCTGCCGTCTCCCAAGGAACTGTCCAGCTTCAGTTTTAGCATGAAAGGCCACCACGAGAA GAACACCAAGTCGAAGACGCGGAGCCTGCTCAAACGCATGGAGAGCCTGAAGCTCAAGGGCTCCCACCAC AGCAAGCACAAGGCGCCTTCCAAGCTGGGGTTGATCATCAGTGCTCCCATTCTGCAGGAGGGTATGGATG AGGCAAAGCTGAAGCAGCTGAACTGTGTGGAGATCTCAGCCCTCAATGGCAACCACATCAACGTGCCCAT GGTACGGAAAAGGAGCGTGTCTAACTCCACCCAGACCAGCAGCAGCAGCAGCCAATCAGAGACCAGCAGC GCGGTCAGCACACCCAGCCCGGTCACCAGGACCCGGAGCCTCAGCACCTGTAACAAGCGGGTGGGCATGT ATCTAGAGGGCTTCGACCCATTCAGTCAGTCCACCTTGAACAACGTGACGGAGCAGAACTATAAAAACCG TGAGAGCTACCCAGAGGACACGGTGTTCTACATTCCCGAAGATCACAAGCCCGGCACCTTCCCTAAGGCC CTCTCCCATGGCAGTTTCTGTCCCTCGGGAAACAGTTCTGTGAACTGGAGGACCGGAAGCTTCCATGGCC CTGGCCATCTCAGCCTACGGAGAGAAAACAGCCATGACAGTCCTAAGGAGCTGAAGAGACGCAATTCTTC CAGCTCTCTGAGCAGCCGCCTGAGCATCTATGATAACGTACCGGGTTCTATCCTGTACTCCAGCTCGGGA GAACTGGCCGACCTGGAGAATGAGGACATCTTCCCTGAGCTGGATGACATTCTCTACCACGTGAAGGGGA TGCAGCGGATAGTCAACCAGTGGTCCGAGAAGTTTTCCGACGAGGGAGACTCGGACTCAGCCCTGGACTC TGTCTCTCCTTGCCCGTCATCTCCAAAACAGATACACCTGGATGTGGACCATGACCGAAGGACACCCAGT GACCTGGACAGCACAGGCAACTCCCTCAATGAGCCCGAAGAGCCCACTGATATCCCGGAAAGAAGAGACT CAGGGGTGGGGGCTTCCCTGACCAGGTGCAATAGGCACAGACTGAGGTGGCACAGCTTCCAGAGCTCCCA CCGGCCGAGCCTCAACTCCGTGTCACTGCAGATTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAG AAATACTCGCTCCTGAAATTGACGGCCCTGCTGGAAAAGTACACGCCTTCCAACAAGCACGGCTTCAGCT GGGCTGTGCCCAAGTTCATGAAAAGGATCAAGGTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCC ACTGACTGTGAACGTGCAGCGCTCAGGACAGCCCCTGCCCCAGAGCATCCAGCAGGCCATGCGCTACCTC CGTAACCACTGTCTGGACCAGGTCGGGCTCTTCAGGAAGTCAGGTGTCAAATCCCGGATCCAGGCTCTAC GCCAGATGAATGAAAGCGCTGAAGATAATGTCAACTATGAAGGCCAGTCTGCTTATGATGTGGCAGACAT GTTAAAGCAATATTTTCGAGATCTTCCTGAGCCCCTCATGACGAACAAACTCTCCGAAACCTTCCTGCAG ATCTACCAGTATGTTCCCAAGGACCAGCGCCTCCAAGCCATCAAGGCGGCCATTATGCTCCTGCCCGACG AGAACCGGGAGGTTCTGCAGACACTTCTCTATTTCCTGAGCGATGTCACAGCGGCTGTGAAAGAAAACCA GATGACTCCCACCAACCTGGCTGTGTGCCTAGCTCCGTCCCTCTTCCACCTCAACACCCTGAAGCGAGAG AATTCTTCTCCAAGGGTAATGCAAAGAAAACAGAGTTTGGGCAAACCAGACCAGAAAGACCTGAATGAAA ACCTAGCGGCGACTCAAGGGCTGGCCCACATGATTGCTGAGTGCAAGAAGCTCTTCCAGGTCCCTGAGGA AATGAGCCGGTGCCGTAACTCCTACACTGAACAAGAGCTGAAGCCCCTTACCCTGGAGGCCTTGGGACAC CTGAATAGTGACCAGCCTGCTGACTACAGACACTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAGGAGG TCAAAGAGAAGTTCAAAGGCTGGGTCAGCTACCCCACCTCCGAACAGGCTGAGCTGTCCTATAAGAAGGT CAGCGAAGGACCCCCGTTAAGGCTTTGGAGGTCAACTATCGAAGTCCCCGCTGCACCCGAGGAGATCTTA AAGCGCCTTCTGAAGGAGCAACACCTCTGGGATGTGGACCTGCTGGACTCCAAGGTGATTGAAATCCTGG ACAGCCAGACTGAAATCTACCAATACGTCCAAAACAGCATGGCGCCCCACCCTGCGCGGGACTACGTCGT ATTGAGGACCTGGAGGACTAACTTACCCAGGGGGGCCTGTGCTCTCTTACTCACCTCTGTGGATCACGAC CGGGCCCCCGTGGCGGGGGTGAGGGTTAATGTGCTCCTGTCCAGATATTTGATTGAACCCTGCGGGTCAG GAAAATCCAAGCTTACCTACATGTGCAGAGCTGATTTAAGGGGCCACATGCCGGAGTGGTACTCAAAATC TTTTGGACATTTGTGTGCAGCCGAAGTGGTAAAGATCCGAGACTCCTTCAGTAATCAGAACACTGAAAGC AAGGACACGAGATCTAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225230 representing NM_001194941
Red=Cloning site Green=Tags(s) MGDPEGHVMARPLRGPLRRSFSDHIRDSTARALDAIWKNTRERRLAEIEAKEACDWLRVTGFPQYAQLYE DLLFPIDIALVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRD SKRWSRLEEFDVFSPKQDPIPGSPDNSRLQSATSHESMLTDLSEHQEVASVRSLSSTSSSVPTHAAHSGD ATTPRTNSVISVCSSGHFVGNDDSFSSLPSPKELSSFSFSMKGHHEKNTKSKTRSLLKRMESLKLKGSHH SKHKAPSKLGLIISAPILQEGMDEAKLKQLNCVEISALNGNHINVPMVRKRSVSNSTQTSSSSSQSETSS AVSTPSPVTRTRSLSTCNKRVGMYLEGFDPFSQSTLNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKA LSHGSFCPSGNSSVNWRTGSFHGPGHLSLRRENSHDSPKELKRRNSSSSLSSRLSIYDNVPGSILYSSSG ELADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDHDRRTPS DLDSTGNSLNEPEEPTDIPERRDSGVGASLTRCNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQ KYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRSGQPLPQSIQQAMRYL RNHCLDQVGLFRKSGVKSRIQALRQMNESAEDNVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQ IYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRE NSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGH LNSDQPADYRHFLQDCVDGLFKEVKEKFKGWVSYPTSEQAELSYKKVSEGPPLRLWRSTIEVPAAPEEIL KRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPRGACALLLTSVDHD RAPVAGVRVNVLLSRYLIEPCGSGKSKLTYMCRADLRGHMPEWYSKSFGHLCAAEVVKIRDSFSNQNTES KDTRSR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001194941 |
ORF Size | 3378 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001194941.1, NP_001181870.1 |
RefSeq Size | 6266 bp |
RefSeq ORF | 3381 bp |
Locus ID | 50768 |
UniProt ID | Q9R0Z9 |
Gene Summary | Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223647 | Dlc1 (untagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 3, (10ug) |
CNY 14,350.00 |
|
MR225230 | Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 3 |
CNY 11,210.00 |
|
MR225230L3 | Lenti ORF clone of Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 3 |
CNY 13,110.00 |
|
MR225230L4 | Lenti ORF clone of Dlc1 (mGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 3 |
CNY 13,110.00 |