United States
Germany
Japan
United Kingdom
China
Dagla (NM_198114) Mouse Tagged ORF Clone
CAT＃: MG224697
- TrueORF®
Dagla (tGFP-tagged) - Mouse diacylglycerol lipase alpha (Dagla), (10ug)
ORF Plasmid: DDK
"NM_198114" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 12,504.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nsddr |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224697 representing NM_198114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGCGATGACCTCGTCCTGCCAGCTATCT TCCTCTTCCTGCTACACACCACCTGGTTTGTAATCCTGTCTGTGGTGCTCTTCGGCTTGGTCTATAACCC ACACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGAGGCTACCTGGGCATCCTTCTGAGCTGTATG ATTGCCGAAATGGCTATCATCTGGCTGAGCATGCGTGGGGGCATCCTCTACACAGAACCCCGGGACTCCA TGCAGTACGTGCTCTACGTGCGCCTGGCCATCTTGGTGATTGAGTTCATCTACGCTATCGTGGGCATCGT CTGGCTCACACAGTACTACACCTCCTGCAATGACCTCACTGCTAAGAATGTCACCCTCGGAATGGTCGTC TGCAACTGGGTGGTCATTCTCAGCGTCTGCATCACTGTCCTCTGCGTCTTTGACCCCACGGGCCGCACCT TCGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGCAATCTGCGGACTTACAACCTGCGGCACCGGTTAGA GGAGGGTCAGGCCACCAGCTGGTCCCGCCGTCTAAAAGTGTTCCTCTGTTGCACCCGGACAAAGGATTCC CAGTCAGATGCCTACTCAGAAATCGCCTACCTCTTTGCTGAATTTTTCCGTGACCTCGACATCGTGCCCT CCGACATCATTGCTGGCCTGGTGCTGCTTAGACAGCGGCAGCGAGCCAAACGCAATGCGGTGCTGGACGA GGCAAACAATGACATCTTGGCTTTCCTGTCTGGGATGCCAGTGACCAGAAACACCAAGTACCTCGACCTC AAGAACTCGCACGAGATGCTACGCTACAAAGAAGTCTGCTACTACATGCTCTTTGCCCTAGCCGCCTATG GCTGGCCCATGTACCTGATGAGGAAGCCCACCTGTGGTCTCTGCCAGCTGGCTCGGTCCTGCTCGTGCTG TCTCTGCCCTGCACGACCCCGATTCGCCCCTGGGGTCACCATTGAGGAAGACAACTGTTGTGGCTGCAAC GCAATCGCTATCCGCCGTCACTTCCTGGATGAGAACATGACTGCTGTAGATATTGTCTACACCTCCTGTC ACGATGCGGTCTATGAAACTCCCTTCTACGTAGCTGTGGACCATGACAAGAAGAAAGTGGTGATCAGTAT CCGGGGAACACTGTCCCCCAAGGATGCCCTGACTGACCTGACAGGCGATGCTGAGCGCCTCCCTGTGGAG GGACACAGAGGCACCTGGTTGGGCCACAAGGGCATGGTACTCTCAGCTGAGTACATCAAGAAGAAGTTGG AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGTGGAACCAAACACTATGGCCTGAT TGTGGTAGGCCACTCCTTGGGAGCAGGCACAGCCGCCATCCTCTCCTTCCTCCTGCGCCCCCAGTACCCG ACCCTCAAGTGCTTCGCTTACTCCCCACCAGGAGGCCTGCTAAGTGAGGATGCTATGGAATACTCCAAAG AGTTTGTGACTGCTGTGGTTCTGGGCAAAGACCTTGTCCCCAGGATTGGCCTTTCCCAGCTGGAAGGTTT CCGTCGGCAGCTTCTGGATGTCCTGCAGCGGAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC AAGTGTATCCCCAAGTCGGAGCTGCCCGAGGATCAGGTGGAGGTGACCACTCTGGCCAGCACACGACTCT GGACACACCCCAGTGATTTGACCATCGCCCTTTCGGCTAGCACCCCACTCTACCCACCTGGCCGCATCAT CCACGTGGTTCACAACCATCCTGCTGAGCAGTGCTGCTGCTGTGAGCAGGAGGAGCCCACATACTTTGCC ATCTGGGGCGACAATAAAGCCTTTAACGAGGTGATCATCTCACCAGCCATGCTGCATGAGCACCTGCCCT ATGTGGTCATGGAGGGGCTCAATAAGGTGCTGGAGAATTACAACAAAGGAAAGACAGCGCTGCTGTCTGC TGCAAAGGTCATGGTGAGCCCCACCGAGGTAGACCTCACTCCCGAGCTCATCTTCCAGCAACAGCCGTTG CCCACGGGCCCACCTCTGCCCACTGGCCTGGCCCTGGAGCTGCCTGCCACAGAGCATCGCAACAGCAGTG TCAGGAGCAAGTCTCAGTCTGAGATGAGTCTGGAGGGCTTCTCTGAGGGCCGGCTGCTGTCCCCAGTGGC TGCTGCATCAGCAGCCCGACAAGACCCCGTAGAGCTTCTGCTGCTGTCCACCCAGGAGCGACTGGCAGCA GAGCTGCAGTCCCGGCGGGCACCACTGGCCACCATGGAGAGCCTCTCAGACACTGAGTCACTGTACAGCT TCGACTCACGCCGCTCCTCAGGCTTCCGAAGCATCAGAGGCTCGCCCAGCCTCCATGCAGTACTGGAGCG TGACGAGGGCCACCTGTTTTACATCGACCCGGCCATTCCCGAGGAGAACCCGTCCCTCAGCTCACGCACA GAGCTGCTGGCAGCCGACAGTCTGTCCAAGCACTCACAGGACACCCAACCCCTGGAGGCGGCTCTAGGCA GCGGGGGTGTGACTCCTGAGCGACCACCAAGTGCAACCATTGAAGAGGAGGAGGCCGCAGGTGGCAGTGA GGGTGGTGGGGTGGCCCCCCGGGGAGAGCTGGCACTGCACAATGGGCGCCTGGGGGACTCACCCAGCCCT CAGGTGCTAGAATTCGCCGAGTTCATTGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAGG ACCTCTACTGCATGATGGTGCCTGAGAGCCCCACTAGTGACTACACTGAGGGCCCCAAGTCCCCCAGCCA ACAGGAAATCTTGCTTCGGGCTCAGTTTGAGCCCAACCTGGTACCCAAGCCCCCCAGGCTCTTTGCAGGA TCTGCTGAACCCTCCTCTGGCATCTCGCTCTCACCCTCCTTCCCACTCAGCTCCTCGGGGGAGCTCATGG ACCTGACCCCCACGGGCCTCAGCAGCCAGGAGTGTCTGGCCACAGACAAGATCCGGACTTCCACCCCCAC AGGCCACGGGGCCAGCCCCACCAAGCAGGATGACCTGGTCATCTCGGCACGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224697 representing NM_198114
Red=Cloning site Green=Tags(s) MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCM IAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVV CNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDS QSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDL KNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCN AIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVE GHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYP TLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGAT KCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFA IWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPL PTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAA ELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRT ELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSP QVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAG SAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198114 |
| ORF Size | 3132 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198114.2, NP_932782.2 |
| RefSeq Size | 5652 bp |
| RefSeq ORF | 3135 bp |
| Locus ID | 269060 |
| UniProt ID | Q6WQJ1 |
| Gene Summary | Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223342 | Dagla (untagged) - Mouse diacylglycerol lipase, alpha (Dagla), (10ug) |
CNY 13,400.00 |
|
| MR224697 | Dagla (Myc-DDK-tagged) - Mouse diacylglycerol lipase, alpha (Dagla) |
CNY 10,904.00 |
|
| MR224697L3 | Lenti ORF clone of Dagla (Myc-DDK-tagged) - Mouse diacylglycerol lipase, alpha (Dagla) |
CNY 10,260.00 |
|
| MR224697L4 | Lenti ORF clone of Dagla (mGFP-tagged) - Mouse diacylglycerol lipase, alpha (Dagla) |
CNY 10,260.00 |
