United States
Germany
Japan
United Kingdom
China
Mdc1 (NM_001010833) Mouse Tagged ORF Clone
CAT＃: MG224467
- TrueORF®
Mdc1 (tGFP-tagged) - Mouse mediator of DNA damage checkpoint 1 (Mdc1), (10ug)
ORF Plasmid: DDK
"NM_001010833" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 15,580.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6820401C03; AA413496; mKIAA0170; NFBD; Nfbd1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224467 representing NM_001010833
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCACCCAGGTGATTGACTGGGATGCTGAAGAAGAGGAAGAGACAGAACTATCCAGTGGCTCCT TGGGGTATAGTGTGGAGCCTATAGGGCAGCTACGTCTCTTCAGTGGAACTCATGGACCAGAAAGAGATTT CCCCCTCTACCTTGGCAAGAATGTAGTTGGTCGAAGCCCTGACTGCTCTGTGGCCCTGCCTTTTCCATCC ATCTCCAAACAGCATGCAGTAATTGAAATCTCAGCTTGGAACAAAGCCCCTATCCTCCAGGATTGTGGGA GCCTCAATGGCACTCAAATCGTAAAGCCTCCTAGGGTCCTACCTCCTGGAGTGAGCCATCGTCTGAGGGA CCAGGAATTAATTCTGTTTGCAGATTTTCCCTGCCAGTACCATCGCCTGGATGTCCCACCACCCTTGGTC CCTCGGAGTCTTCTAACTATAGAGAAAACCCCCAGAATACGGATAGAATCCCAAAATTCCAGAGTTTTGT TGGCTGCCGATTCAGAGGAAGAAGGGGATTTTCCTTCTGGAAGGTGTGTGGCGAATGGACAAAGGAATAC AGCATCCCCTTCAGCAACAGTAGTTCCAGAAAGTGATGAAGAGGTCTCTTCTCCGGCCCCAAGTGTCCCC GGGCCATCTTCACCCTTTGGTTTGGGCAGTGACACTGATGAAGAACAAGGTCAGCAACCAGGAGTAGAGG AGTCCTCTTTAGCTGACAGCAGTGGTGCTGCAGGGGAAGCTGAGCAGCCTGAAGCTAACGGAACGACAGC TGGCATACAGGCTCAGCCTACTGAGCACAAATTGAAGGACACAAAAGTCAAGAAGGAGGCAGGCAGGGCA GGGGTCTCAGATGGGTCAGTTCTGGAGAGGAGCCCCACTCTTGGAGAGGACAGTGACACAGAAGTGGACG AAGATCACAAACCTGGGTTTGCGGACAGTGAGACAGATGTGGAAGAAGAGAGGATCCCTGTGACCCCCCC TGTAGCCCCTGTGAAGAAAAACCAAGTGCTACTTGCAGTTGGTATAGGAGATCCTGAAGCACCTGGTGTG GCACATCTGCAGGACTGCCTAGCTGGTAGTGGCACAGATGTGGAGGACAAGACTGCACTGGATGTCCCTC TAGAGAGAAACCACACACCCATGGTGATCAACAGTGATACAGATGAGGAGGAGGAGGAGGAGGAGGAGGA GGTCTCAGCAGCACTCACTTTGGCCCATCTGAAAGAGAGAGGAATTGGTTTGTGGAGTAGAGACCCAGGC GCAGAAGAGGTCAAGTCCCAACCACAGGTCCTTGTAGAACAAAGCCAGAGTGCCTCTGGGAGAGACAGTG ACACGGATGTGGAGGAGGAATCCTCAGGGCGAAAGAGAGAGATTATCCCAGACAGTCCCATGGATGTAGA TGAGGCTCTTACTGTCACACAGCCAGAGAGCCAGCCTCCCCGTAGGCCCAATGATGCAGATGAGTACATG GATATGAGCTCCCCTGGCAGCCATCTAGTGGTAAATCAGGCCTCCTTTGCTGTGGTAGGCAAGACCAGAG CACAAGTGGAGGAGGAAGTCCCAGGGCCATCTGTTATCCTGGGGGAGAAGCACCAGGTGCCTCTAGAGGG AGCCCAGCCTCCTGAGGAAGCCTGGGAAACAGCTGTTCAGGAAGGCTCATCCTCACCAGAGGCAGCAGCC TCTGTAAGGCCTAGCCAGCAGCCAGTAGCAGAAGATGCTGGGACAGAGTGTGCTACAGCTGTTTCTGAGC AGGAAAGTACTCTTGAGGTGAGGTCCCAAAGCGGGTCACCTGCAGCACCAGTGGAGCAAGTGGTGATACA TACAGATACTTCAGGGGATCCCACCCTGCCACAGAGAGAGGGAGCCCAAACCCCCACAGGAAGGGAGAGA GAAGCACATGTGGGCAGGACCAAGAGTGCCAAAGAGTGCTGTGATGCAGAACCTGAAGATCTGTGCCTTC CAGCTACCCAGTGCTTTGTGGAAGGGGAGAGCCAGCACCCAGAAGCTGTCCAGAGTTTGGAAAATGAGCC TACCCAACTCTTCCCATGTACTCTTCCACAAGAGCCAGGACCTTCCCATCTCAGCCTTCAGACTCCAGGT GCAGATACCTTGGATGTGCCTTGGGAAGTCTTGGCTACACAGCCGTTCTGTCTAAGAGAACAGTCAGAGA CTTCTGAGCTTCATGAAGCTCATGGATCTCAACCATCTCTGCCTAGGGAACCACCAGGACACCAACATCT GGTTCATACCAGCCCAGTTCACACAGAGCTGTTGAGAATTGAAGGCAGAGAGATACAGACTGTGGAGAAA GCCATGGGTATACCAAAGGAAATGGCAGACAGGATGACCCCTGAGAGAGAGCCATTGGAGAGGGAAATCA GGGGAAGAACAGAAAACAGTGAGAGAGATGTGATAGGAGAAGAACTAATTCAGGGGACAAAGGACAGAGA GCCAAAGAAGGTGTTAGCTAGAGACAGTCAGAGAAAAGAGGCTGACAAGGATCTTGAAGGGAATAGGGAG AGTTTGGAGGTAGAAATAGAGATGTCCAAAGACTCACAGAAGAGAGAGAGAAAAGTAGAGAAACCAGAAC CAAAGAGAGAATGGGAGCCAGCTGATTTAGAAGTGACTCCAGATAGAGGGGTGACAGAGGAAGGGAGCCA TGATCAGAAAGGGCAAATTGCTAGTCTAACACTAAAGCCTGGAGTTGGGGTGAAGGACCTTGAGGGACTT GCATCAGCCCCAATAATCACAGGGAGCCAGGCAGATGGAGGAAAGGGAGACCCTTTGAGCCCTGGGAGGC AGCAGAGAGGCCGCTTGAGTTGCCAGACGACACCTGCTGGAAAGGCTTCCAGGGGTGATCCAGAGCCCCC AGACCACTGCCTGTTTTCTTCAGTGCCTGAAGCTTCAACTCAGAGTCTCCTCACCTCTCAGAGCCAAAAG CAGTCTACACCTCAGCCTCTGTTTTCGACCTCTTCTTCTGAGATACCCCTTCCTGAGAGTCTTCACACAA AGCCTAATGTCAGGCCACGGCGGTCCTCCAGGATGACCCCCTCCCCGCATTCCTCTGCTGCCCTTAAGCC AAATACTACCTGCCCCACAAACCAGCCTGCTGCCTCTAGACCAACATCCCGACCCACTCGGGGCAGGGCA AATAGGTCCTCTACCAGGACCCCAGAACTGATTGTCCCTGTAGACCCTGAACTCCAGCCTTCCACCTCCA CAGAACAGCCTGTCATCCCAAAACTTACGTCCCAGGTCACTGAGGGCAGGGTACAGATGCCTGAACCACT TCTCACAGGTCCCGAGATCCAGTCTCCCACCTCCACAGAACAGTCTGTCACCCCAGACCGCAAACCTCGG GCCACTCGGGGCAGGCCAAGTAAATCTCCCAACAAGACCCCAGAACCACTTATTTCTACTGGCCCTGAAC TCCAACCTCCCACCTCCATAGAACAACCTGTCATACCTAAACCCACTTCTCGGGTCACTCGGGGCAGACC ACGTAAGTCTTCTGTCAGGACCCCAGAATCAGTTGTCTCCACTGGCCCTGAACTTCAGCCTCTCACCTCT ATAGAACAGCCTGTTATCCCTGAACCTAGGGCCACTCGAGGTAGACCAAGTAAGTCTTCTATCAAGACCC CAGAATCAGTTGTCCCCACTGGTCCTGAGCTGCAGCCTCTTACCTCTGCAAAACAGCCTGTCACCCCTAA CCTCACATCTCGGGCCTCTCGGGGTAGATCAAGTAAGTCTATCAGGACCCCGGAACCAGTTGTCCAAACT GGCCCTGAATTCCATCCATCCACCTCCACAGAACAGCCTGATACCCGTGAGCCCTCATCTCAGGCTAGGA CACGTAGGTCTGCTGTCAAGACTCCTGAAGCGAGTGTCCCCACAACCCCTGAACTCCAGCCTTTCACTTC TAAAAAACAGCCTGCCCCTAAGCCTACAGCTCTGGTCACTCAGGGAAGGACATATAAACCCTCCACTGAA GATTGTGAATCAGTTGGGCCAGTGGCCCCTGATTTTGAGCCTTCCACCTCTACAGACCATCTTGTCACCC CTAAGGTTACAGATCAGAGCTTAACACTACAGTCTTCACCCCTAAGTGCTTCCCCAGTTTCCAGTACCCC TGATCTCAAGCCTCCTGTCCCCATAGCCCAGCCTGTTACCCCAGAGCCCATTCCACAAGCCAATCACCAA AGGAAACGAAGGGCTGCTGGGAAGCAGGGTTCCCGCACAGTCCCTCTGGGTCATAAGTCTTACTCTGCCC TCTCTGAACCTGAGCCCCAATCTTCAGCCAGCCAAAGCTCAGGAGCATCAGAAGCAGACTCACCCCGTCA AAAACGTCCCAGAAGACAAGCCTCCCAGAAAACTGTAGTCATCAAGGAAGAACCTGTAGAAACAGAAGTG AAGGAAGAGCCTCAAGAGACTGCAATTCCAACACCAGAAAAGAGAAAGAGGGACCATGCAGAAGAAGTGA CCCAGGGAAAACCAACTCGAAGCCGGCGAACTAAACCTAACCAAGAAACAGCCCCCAAGGTACTGTTCAC AGGAGTCATGGATTCTCGTGGCGAGCGTGCAGTGCTGGCTCTGGGAGGCAGTCTGGCCAGCTCAGTAAAT GAGGCCTCCCACTTAGTTACTGATCGCATCCGCAGGACAGTCAAGTTCTTGTGTGCCCTGGGGAAGGGAA TTCCCATCCTGTCCCTGAACTGGCTGTACCAGTCCCGAAAGGCTGGTTGCTTCTTGCCGCCTGATGACTA CTTAGTGACTGATCCTGAGCAAGAGAAGAACTTTAGCTTCAGCCTTCGGGATTCCCTGTGCCGGGCTCGG GAACGAAGACTGCTGGAGGACTATGAGATTCATGTGACCCCTGGCGTGCAGCCACCCCCCCCTCAGATGG GCGAGATCATCAGTTGCTGTGGAGGCACTTTCCTGCCCAGCATGCCTCACTCCTATAAGCTTCACCGAGT CATCATAACTTGCACTGAAGACCTACCACGCTGTGCTATTCCATCCCGCCTGGGGCTACCCCTCCTCTCT CCTGAGTTCCTCCTGACTGGAGTGCTGAAGCAGGAAGCCACACCAGAGGCCTTTGTTCTCTCCAATTTGG AAATGTCATCTACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224467 representing NM_001010833
Red=Cloning site Green=Tags(s) MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPS ISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLV PRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVP GPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRA GVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGV AHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPG AEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYM DMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAA SVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGRER EAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPG ADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEK AMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRE SLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGL ASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQK QSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRA NRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPR ATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTS IEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQT GPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTE DCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQ RKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEV KEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVN EASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRAR ERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLS PEFLLTGVLKQEATPEAFVLSNLEMSST TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001010833 |
| ORF Size | 5124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001010833.2, NP_001010833.2 |
| RefSeq Size | 7372 bp |
| RefSeq ORF | 5127 bp |
| Locus ID | 240087 |
| UniProt ID | Q5PSV9 |
| Gene Summary | The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
