Mdc1 (NM_001010833) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6820401C03; AA413496; mKIAA0170; NFBD; Nfbd1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224851 representing NM_001010833 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCACCCAGGTGATTGACTGGGATGCTGAAGAAGAGGAAGAGACAGAACTATCCAGTGGCTCCT TGGGGTATAGTGTGGAGCCTATAGGGCAGCTACGTCTCTTCAGTGGAACTCATGGACCAGAAAGAGATTT CCCCCTCTACCTTGGCAAGAATGTAGTTGGTCGAAGCCCTGACTGCTCTGTGGCCCTGCCTTTTCCATCC ATCTCCAAACAGCATGCAGTAATTGAAATCTCAGCTTGGAACAAAGCCCCTATCCTCCAGGATTGTGGGA GCCTCAATGGCACTCAAATCGTAAAGCCTCCTAGGGTCCTACCTCCTGGAGTGAGCCATCGTCTGAGGGA CCAGGAATTAATTCTGTTTGCAGATTTTCCCTGCCAGTACCATCGCCTGGATGTCCCACCACCCTTGGTC CCTCGGAGTCTTCTAACTATAGAGAAAACCCCCAGAATACGGATAGAATCCCAAAATTCCAGAGTTTTGT TGGCTGCCGATTCAGAGGAAGAAGGGGATTTTCCTTCTGGAAGGTGTGTGGCGAATGGACAAAGGAATAC AGCATCCCCTTCAGCAACAGTAGTTCCAGAAAGTGATGAAGAGGTCTCTTCTCCGGCCCCAAGTGTCCCC GGGCCATCTTCACCCTTTGGTTTGGGCAGTGACACTGATGAAGAACAAGGTCAGCAACCAGGAGTAGAGG AGTCCTCTTTAGCTGACAGCAGTGGTGCTGCAGGGGAAGCTGAGCAGCCTGAAGCTAACGGAACGACAGC TGGCATACAGGCTCAGCCTACTGAGCACAAATTGAAGGACACAAAAGTCAAGAAGGAGGCAGGCAGGGCA GGGGTCTCAGATGGGTCAGTTCTGGAGAGGAGCCCCACTCTTGGAGAGGACAGTGACACAGAAGTGGACG AAGATCACAAACCTGGGTTTGCGGACAGTGAGACAGATGTGGAAGAAGAGAGGATCCCTGTGACCCCCCC TGTAGCCCCTGTGAAGAAAAACCAAGTGCTACTTGCAGTTGGTATAGGAGATCCTGAAGCACCTGGTGTG GCACATCTGCAGGACTGCCTAGCTGGTAGTGGCACAGATGTGGAGGACAAGACTGCACTGGATGTCCCTC TAGAGAGAAACCACACACCCATGGTGATCAACAGTGATACAGATGAGGAGGAGGAGGAGGAGGAGGAGGA GGTCTCAGCAGCACTCACTTTGGCCCATCTGAAAGAGAGAGGAATTGGTTTGTGGAGTAGAGACCCAGGC GCAGAAGAGGTCAAGTCCCAACCACAGGTCCTTGTAGAACAAAGCCAGAGTGCCTCTGGGAGAGACAGTG ACACGGATGTGGAGGAGGAATCCTCAGGGCGAAAGAGAGAGATTATCCCAGACAGTCCCATGGATGTAGA TGAGGCTCTTACTGTCACACAGCCAGAGAGCCAGCCTCCCCGTAGGCCCAATGATGCAGATGAGTACATG GATATGAGCTCCCCTGGCAGCCATCTAGTGGTAAATCAGGCCTCCTTTGCTGTGGTAGGCAAGACCAGAG CACAAGTGGAGGAGGAAGTCCCAGGGCCATCTGTTATCCTGGGGGAGAAGCACCAGGTGCCTCTAGAGGG AGCCCAGCCTCCTGAGGAAGCCTGGGAAACAGCTGTTCAGGAAGGCTCATCCTCACCAGAGGCAGCAGCC TCTGTAAGGCCTAGCCAGCAGCCAGTAGCAGAAGATGCTGGGACAGAGTGTGCTACAGCTGTTTCTGAGC AGGAAAGTACTCTTGAGGTGAGGTCCCAAAGCGGGTCACCTGCAGCACCAGTGGAGCAAGTGGTGATACA TACAGATACTTCAGGGGATCCCACCCTGCCACAGAGAGAGGGAGCCCAAACCCCCACAGGAAGGGAGAGA GAAGCACATGTGGGCAGGACCAAGAGTGCCAAAGAGTGCTGTGATGCAGAACCTGAAGATCTGTGCCTTC CAGCTACCCAGTGCTTTGTGGAAGGGGAGAGCCAGCACCCAGAAGCTGTCCAGAGTTTGGAAAATGAGCC TACCCAACTCTTCCCATGTACTCTTCCACAAGAGCCAGGACCTTCCCATCTCAGCCTTCAGACTCCAGGT GCAGATACCTTGGATGTGCCTTGGGAAGTCTTGGCTACACAGCCGTTCTGTCTAAGAGAACAGTCAGAGA CTTCTGAGCTTCATGAAGCTCATGGATCTCAACCATCTCTGCCTAGGGAACCACCAGGACACCAACATCT GGTTCATACCAGCCCAGTTCACACAGAGCTGTTGAGAATTGAAGGCAGAGAGATACAGACTGTGGAGAAA GCCATGGGTATACCAAAGGAAATGGCAGACAGGATGACCCCTGAGAGAGAGCCATTGGAGAGGGAAATCA GGGGAAGAACAGAAAACAGTGAGAGAGATGTGATAGGAGAAGAACTAATTCAGGGGACAAAGGACAGAGA GCCAAAGAAGGTGTTAGCTAGAGACAGTCAGAGAAAAGAGGCTGACAAGGATCTTGAAGGGAATAGGGAG AGTTTGGAGGTAGAAATAGAGATGTCCAAAGACTCACAGAAGAGAGAGAGAAAAGTAGAGAAACCAGAAC CAAAGAGAGAATGGGAGCCAGCTGATTTAGAAGTGACTCCAGATAGAGGGGTGACAGAGGAAGGGAGCCA TGATCAGAAAGGGCAAATTGCTAGTCTAACACTAAAGCCTGGAGTTGGGGTGAAGGACCTTGAGGGACTT GCATCAGCCCCAATAATCACAGGGAGCCAGGCAGATGGAGGAAAGGGAGACCCTTTGAGCCCTGGGAGGC AGCAGAGAGGCCGCTTGAGTTGCCAGACGACACCTGCTGGAAAGGCTTCCAGGGGTGATCCAGAGCCCCC AGACCACTGCCTGTTTTCTTCAGTGCCTGAAGCTTCAACTCAGAGTCTCCTCACCTCTCAGAGCCAAAAG CAGTCTACACCTCAGCCTCTGTTTTCGACCTCTTCTTCTGAGATACCCCTTCCTGAGAGTCTTCACACAA AGCCTAATGTCAGGCCACGGCGGTCCTCCAGGATGACCCCCTCCCCGCATTCCTCTGCTGCCCTTAAGCC AAATACTACCTGCCCCACAAACCAGCCTGCTGCCTCTAGACCAACATCCCGACCCACTCGGGGCAGGGCA AATAGGTCCTCTACCAGGACCCCAGAACTGATTGTCCCTGTAGACCCTGAACTCCAGCCTTCCACCTCCA CAGAACAGCCTGTCATCCCAAAACTTACGTCCCAGGTCACTGAGGGCAGGGTACAGATGCCTGAACCACT TCTCACAGGTCCCGAGATCCAGTCTCCCACCTCCACAGAACAGTCTGTCACCCCAGACCGCAAACCTCGG GCCACTCGGGGCAGGCCAAGTAAATCTCCCAACAAGACCCCAGAACCACTTATTTCTACTGGCCCTGAAC TCCAACCTCCCACCTCCATAGAACAACCTGTCATACCTAAACCCACTTCTCGGGTCACTCGGGGCAGACC ACGTAAGTCTTCTGTCAGGACCCCAGAATCAGTTGTCTCCACTGGCCCTGAACTTCAGCCTCTCACCTCT ATAGAACAGCCTGTTATCCCTGAACCTAGGGCCACTCGAGGTAGACCAAGTAAGTCTTCTATCAAGACCC CAGAATCAGTTGTCCCCACTGGTCCTGAGCTGCAGCCTCTTACCTCTGCAAAACAGCCTGTCACCCCTAA CCTCACATCTCGGGCCTCTCGGGGTAGATCAAGTAAGTCTATCAGGACCCCGGAACCAGTTGTCCAAACT GGCCCTGAATTCCATCCATCCACCTCCACAGAACAGCCTGATACCCGTGAGCCCTCATCTCAGGCTAGGA CACGTAGGTCTGCTGTCAAGACTCCTGAAGCGAGTGTCCCCACAACCCCTGAACTCCAGCCTTTCACTTC TAAAAAACAGCCTGCCCCTAAGCCTACAGCTCTGGTCACTCAGGGAAGGACATATAAACCCTCCACTGAA GATTGTGAATCAGTTGGGCCAGTGGCCCCTGATTTTGAGCCTTCCACCTCTACAGACCATCTTGTCACCC CTAAGGTTACAGATCAGAGCTTAACACTACAGTCTTCACCCCTAAGTGCTTCCCCAGTTTCCAGTACCCC TGATCTCAAGCCTCCTGTCCCCATAGCCCAGCCTGTTACCCCAGAGCCCATTCCACAAGCCAATCACCAA AGGAAACGAAGGGCTGCTGGGAAGCAGGGTTCCCGCACAGTCCCTCTGGGTCATAAGTCTTACTCTGCCC TCTCTGAACCTGAGCCCCAATCTTCAGCCAGCCAAAGCTCAGGAGCATCAGAAGCAGACTCACCCCGTCA AAAACGTCCCAGAAGACAAGCCTCCCAGAAAACTGTAGTCATCAAGGAAGAACCTGTAGAAACAGAAGTG AAGGAAGAGCCTCAAGAGACTGCAATTCCAACACCAGAAAAGAGAAAGAGGGACCATGCAGAAGAAGTGA CCCAGGGAAAACCAACTCGAAGCCGGCGAACTAAACCTAACCAAGAAACAGCCCCCAAGGTACTGTTCAC AGGAGTCATGGATTCTCGTGGCGAGCGTGCAGTGCTGGCTCTGGGAGGCAGTCTGGCCAGCTCAGTAAAT GAGGCCTCCCACTTAGTTACTGATCGCATCCGCAGGACAGTCAAGTTCTTGTGTGCCCTGGGGAAGGGAA TTCCCATCCTGTCCCTGAACTGGCTGTACCAGTCCCGAAAGGCTGGTTGCTTCTTGCCGCCTGATGACTA CTTAGTGACTGATCCTGAGCAAGAGAAGAACTTTAGCTTCAGCCTTCGGGATTCCCTGTGCCGGGCTCGG GAACGAAGACTGCTGGAGGACTATGAGATTCATGTGACCCCTGGCGTGCAGCCACCCCCCCCTCAGATGG GCGAGATCATCAGTTGCTGTGGAGGCACTTTCCTGCCCAGCATGCCTCACTCCTATAAGCTTCACCGAGT CATCATAACTTGCACTGAAGACCTACCACGCTGTGCTATTCCATCCCGCCTGGGGCTACCCCTCCTCTCT CCTGAGTTCCTCCTGACTGGAGTGCTGAAGCAGGAAGCCACACCAGAGGCCTTTGTTCTCTCCAATTTGG AAATGTCATCTACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001010833
Insert Size	5127 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001010833.2, NP_001010833.2
RefSeq Size	7372 bp
RefSeq ORF	5127 bp
Locus ID	240087
UniProt ID	Q5PSV9
Gene Summary	The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008]

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