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Agrn (NM_021604) Mouse Tagged ORF Clone
CAT＃: MG224120
- TrueORF®
Agrn (tGFP-tagged) - Mouse agrin (Agrn), (10ug)
ORF Plasmid: DDK
"NM_021604" in other vectors (2)
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CNY 22,520.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Agrin; nmf380 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224120 representing NM_021604
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGCCTCCTGATGTGTGCAGGGGAATGTTATGTGGCTTTGGTGCTGTGTGTGAACCTAGTGTTGAGGATC CAGGCCGGGCCTCCTGTGTGTGCAAGAAGAATGTCTGCCCTGCTATGGTAGCTCCTGTGTGTGGCTCAGA TGCTTCCACCTATAGCAACGAGTGTGAGCTACAGCGTGCACAGTGCAACCAGCAACGGCGCATCCGCCTA CTCCGCCAAGGGCCATGTGGGTCCCGGGACCCCTGTGCCAATGTGACCTGCAGTTTCGGTAGTACCTGTG TACCTTCAGCCGATGGACAGACCGCCTCGTGTCTGTGTCCTACAACCTGCTTTGGGGCCCCTGATGGCAC AGTGTGTGGCAGTGATGGTGTTGACTACCCTAGTGAGTGCCAGCTGCTCCGTCATGCCTGTGCCAACCAG GAGCACATCTTTAAGAAGTTCGATGGTCCTTGTGACCCCTGCCAGGGCAGCATGTCAGACCTGAATCATA TTTGCCGGGTGAACCCACGTACACGGCACCCAGAAATGCTTCTGCGGCCTGAGAACTGCCCCGCCCAACA CACACCTATCTGTGGAGATGATGGGGTCACCTATGAAAACGACTGTGTCATGAGCCGTATAGGTGCAGCC CGTGGCCTGCTTCTCCAGAAAGTGCGCTCTGGTCAATGCCAGACTCGAGACCAGTGCCCGGAGACCTGCC AGTTTAACTCTGTATGCCTGTCCCGCCGCGGCCGTCCCCACTGTTCCTGCGATCGCGTCACCTGTGATGG GGCTTACAGGCCAGTGTGTGCCCAGGATGGGCACACGTATGACAATGACTGTTGGCGCCAACAGGCCGAG TGTCGACAACAGCAGACCATTCCCCCCAAGCACCAGGGCCCGTGTGACCAGACCCCATCCCCGTGCCGTG GAGCGCAGTGTGCATTTGGGGCAACATGCACAGTGAAGAATGGGAAAGCTGTGTGCGAGTGCCAGCGGGT GTGCTCGGGCGGCTACGATCCTGTGTGCGGCAGTGATGGTGTCACTTACGGCAGTGTGTGCGAGCTGGAA TCCATGGCCTGTACCCTTGGGCGGGAAATCCGAGTGGCCCGCAGAGGACCGTGTGACCGATGTGGGCAGT GCCGGTTTGGATCCTTGTGCGAGGTGGAGACTGGACGCTGTGTGTGCCCCTCTGAGTGTGTGGAGTCAGC CCAGCCCGTATGTGGCTCTGACGGACACACATATGCTAGTGAATGTGAGCTGCATGTCCACGCCTGTACA CACCAGATCAGCCTATACGTGGCCTCAGCCGGACACTGCCAGACCTGTGGAGAAACAGTTTGTACCTTTG GGGCTGTGTGCTCAGCTGGACAGTGTGTATGTCCCCGTTGTGAGCACCCCCCACCTGGCCCTGTGTGCGG CAGTGATGGCGTCACCTACCTCAGTGCCTGTGAGCTCCGAGAGGCTGCCTGTCAGCAGCAGGTACAAATT GAGGAGGCCCGTGCAGGGCCGTGTGAGCCGGCTGAGTGTGGCTCAGGGGGCTCTGGGTCTGGGGAAGACA ATGCGTGTGAGCAGGAGCTGTGTCGGCAGCATGGTGGTGTCTGGGATGAGGACTCAGAAGACGGGCCGTG TGTCTGTGACTTTAGTTGCCAGAGTGTCCTTAAAAGCCCAGTGTGTGGCTCAGATGGAGTCACCTATAGC ACGGAGTGCCATCTGAAGAAGGCCAGATGTGAAGCGCGGCAAGAGCTGTACGTCGCTGCTCAGGGAGCCT GCCGGGGCCCTACCTTGGCTCCACTGCTACCTATGGCCTCCCCACACTGTGCCCAAACCCCCTATGGCTG CTGCCAGGACAATGTCACTGCTGCCCAGGGTGTGGGCTTGGCTGGCTGTCCCAGCACCTGCCATTGCAAC CCACACGGCTCCTATAGCGGCACTTGTGACCCAGTCACAGGGCAGTGCTCCTGCCGACCAGGTGTAGGAG GCCTCAGGTGTGATCGCTGTGAGCCTGGCTTCTGGAACTTCCGTGGCATTGTCACCGATGGACATAGTGG TTGCACTCCCTGCAGCTGTGACCCTCGGGGTGCTGTAAGAGATGACTGTGAGCAGATGACTGGATTGTGT TCCTGTAGACCTGGTGTGGCTGGTCCCAAGTGTGGGCAGTGTCCAGATGGTCAAGCCCTGGGCCATCTTG GCTGTGAAGCAGATCCCACAACACCAGTGACTTGTGTGGAAATGCACTGTGAGTTTGGCGCCTCCTGCGT AGAGGAGGCTGGTTTTGCCCAGTGTGTCTGCCCAACTCTCACATGTCCAGAGGCTAACTCTACCAAGGTC TGTGGATCAGATGGTGTCACATACGGCAATGAATGCCAGCTGAAGACCATTGCCTGCCGCCAGCGTCTGG ACATCTCCATTCAGAGTCTTGGTCCATGCCGGGAGAGTGTTGCTCCTGGGGTTTCCCCTACATCTGCATC TATGACCACCCCAAGGCATATCCTGAGCAGGACACTGGCGTCTCCCCACAGCAGCCTTCCTCTGTCTCCC AGCACTACTGCCCATGATTGGCCCACCCCATTACCCACATCACCTCAGACCGTAGTCGGCACCCCCAGGA GCACTGCAGCCACACCCTCTGATGTGGCCAGTCTTGCTACAGCGATCTTCAGGGAATCTGGCAGCACCAA CGGCAGTGGCGATGAGGAGCTCAGTGGCGATGAGGAGGCCAGTGGGGGCGGGTCTGGGGGACTTGAGCCC CCGGTGGGCAGCGTTGTGGTGACCCACGGGCCACCCATCGAGAGGGCTTCCTGTTACAACTCACCTTTGG GCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCAGAAGGCTCCAACTGTCCAGCTACCAAGGCATT CCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCAGAGATGGCTGACCCC AAGTCAGAGTTGTTTGGGGAGACTGCAAGGAGCATTGAGAGCACGCTGGACGACCTGTTCCGGAATTCGG ATGTTAAGAAGGACTTCTGGAGCATCCGCCTACGGGAACTGGGGCCTGGCAAATTAGTCCGTGCCATTGT GGATGTTCACTTTGACCCCACCACAGCCTTCCAGGCACCAGATGTGGGTCAGGCCTTGCTCCAACAGATC CAGGTATCCAGGCCGTGGGCCCTGGCAGTGAGGAGGCCTCTGCGGGAGCATGTGCGATTCTTGGACTTTG ACTGGTTTCCCACTTTTTTTACGGGAGCTGCAACAGGAACCACAGCTGCTGTGGCCACAGCCAGAGCCAC CACTGTGAGCCGACTGTCTGCCTCTTCTGTCACCCCACGAGTCTACCCCAGTTACACCAGCCGGCCTGTT GGCAGAACTACGGCACCGCTAACCACTCGCCGGCCACCAACCACTACCGCCAGTATTGACCGACCTCGGA CTCCAGGCCCGCAACGGCCCACAAAGTCCTGTGATTCCCAGCCTTGCCTCCACGGAGGTACCTGCCAGGA CCTGGATTCTGGCAAGGGTTTCAGCTGCAGCTGTACTGCAGGCAGGGCTGGCACTGTCTGTGAGAAAGTG CAACTCCCCTCTGTGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCTCCGAGCCTACCACA CGCTGCGTCTGGCACTAGAATTCCGGGCGCTGGAGACAGAGGGACTGCTGCTCTACAATGGCAATGCACG TGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGTCATGTACAGTTCAGGTTCGACACGGGCTCAGGG CCGGCGGTGCTAACAAGCTTAGTGCCAGTGGAACCGGGACGGTGGCACCGCCTCGAGTTGTCACGGCATT GGCGGCAGGGCACACTTTCTGTGGATGGCGAGGCTCCTGTTGTAGGTGAAAGTCCGAGTGGCACTGATGG CCTCAACTTGGACACGAAGCTCTATGTGGGTGGTCTCCCAGAAGAACAAGTTGCCACGGTGCTTGATCGG ACCTCTGTGGGCATCGGCCTGAAAGGATGCATTCGTATGTTGGACATCAACAACCAGCAGCTGGAGCTGA GCGATTGGCAGAGGGCTGTGGTTCAAAGCTCTGGTGTGGGGGAATGCGGAGACCATCCCTGCTCACCTAA CCCCTGCCATGGCGGGGCCCTCTGCCAGGCCCTGGAGGCTGGCGTGTTCCTCTGTCAGTGCCCACCTGGC CGCTTTGGCCCAACTTGTGCAGATGAAAAGAACCCCTGCCAACCGAACCCCTGCCACGGGTCAGCCCCCT GCCATGTGCTTTCCAGGGGTGGGGCCAAGTGTGCGTGCCCCCTGGGACGCAGTGGTTCCTTCTGTGAGAC AGTCCTGGAGAATGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCTACCTGGAACTGAAA GGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTTCTTGGCTCGTGGGC CCAGTGGCTTACTCCTCTACAATGGGCAGAAGACGGATGGCAAGGGGGACTTTGTATCCCTGGCCCTGCA TAACCGGCACCTAGAGTTCCGCTATGACCTTGGCAAGGGGGCTGCAATCATCAGGAGCAAAGAGCCCATA GCCCTGGGCACCTGGGTTAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCTTCAAGTGGGTGATG GGCCCCGTGTGCTAGGGGAATCTCCGGTCCCGCACACCATGCTCAACCTCAAGGAGCCCCTCTATGTGGG GGGAGCTCCTGACTTCAGCAAGCTGGCTCGGGGCGCTGCAGTGGCCTCCGGCTTTGATGGTGCCATCCAG CTGGTGTCTCTAAGAGGCCATCAGCTGCTGACTCAGGAGCATGTGTTGCGGGCAGTAGATGTAGCGCCTT TTGCAGGCCACCCTTGTACCCAGGCCGTGGACAACCCCTGCCTTAATGGGGGCTCCTGTATCCCGAGGGA AGCCACTTATGAGTGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAGAAGGGGATAGTTGAGAAG TCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAGTACCTCAATGCTGTGACTG AGAGTGAGAAAGCGCTGCAGAGCAACCACTTTGAGCTGAGCTTACGCACTGAGGCCACGCAGGGGCTGGT GCTGTGGATTGGAAAGGTTGGAGAACGTGCAGACTACATGGCTCTGGCCATTGTGGATGGGCACCTACAA CTGAGCTATGACCTAGGCTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAACACCAACCGCTGGC TTCGAGTCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAAGCCCCTGTGACTGG CTCTTCCCCGCTGGGTGCCACACAATTGGACACAGATGGAGCCCTGTGGCTTGGAGGCCTACAGAAGCTT CCTGTGGGGCAGGCTCTCCCCAAGGCCTATGGCACGGGTTTTGTGGGCTGTCTGCGGGACGTGGTAGTGG GCCATCGCCAGCTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCCTGCCCCACTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224120 representing NM_021604
Red=Cloning site Green=Tags(s) MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRL LRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQ EHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAA RGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAE CRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELE SMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACT HQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQI EEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYS TECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCN PHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLC SCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKV CGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSP STTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEP PVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADP KSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQI QVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPV GRTTAPLTTRRPPTTTASIDRPRTPGPQRPTKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKV QLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSG PAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDR TSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPG RFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELK GLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPI ALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQ LVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEK SVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQ LSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKL PVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL TRTRPLE - GFP Tag - V |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021604 |
| ORF Size | 5598 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021604.2, NP_067617.2 |
| RefSeq Size | 7379 bp |
| RefSeq ORF | 6105 bp |
| Locus ID | 11603 |
| Gene Summary | Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.[UniProtKB/Swiss-Prot Function] |
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