Agrn (NM_021604) Mouse Untagged Clone

CAT#: MC224986

Agrn (untagged) - Mouse agrin (Agrn), (10ug)



  "NM_021604" in other vectors (2)

CNY 29,930.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Agrin; nmf380
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224986 representing NM_021604
Red=Cloning site Blue=ORF

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGTCCGCCCGCGGCTGAGCTTCCCGGCCCCGCTGCTGCCGCTGCTGCTGCTCCTGGCGGCGGCCGCGC
CAGCCGTGCCGGGCGCCAGCGGGACGTGCCCGGAGCGCGCCTTGGAGCGGCGCGAGGAGGAGGCGAACGT
GGTGCTCACCGGCACAGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACCTACTCCTGCAAGGTT
CGAGTGTGGCGGTACTTGAAAGGCAAAGATGTGGTGGCCCAAGAGAGCCTGCTGGATGGTGGCAACAAGG
TGGTAATTGGTGGCTTTGGAGACCCCCTCATCTGCGACAACCAGGTGTCCACCGGGGACACTAGAATCTT
CTTTGTGAACCCAGCACCACCCTACCTGTGGCCAGCCCACAAGAATGAGTTGATGCTCAACTCCAGCCTT
ATGCGGATCACTTTGCGGAACCTGGAAGAGGTGGAGTTCTGTGTAGAAGACAAACCTGGGATCCACTTCA
CAGCAGCGCCTTCTATGCCTCCTGATGTGTGCAGGGGAATGTTATGTGGCTTTGGTGCTGTGTGTGAACC
TAGTGTTGAGGATCCAGGCCGGGCCTCCTGTGTGTGCAAGAAGAATGTCTGCCCTGCTATGGTAGCTCCT
GTGTGTGGCTCAGATGCTTCCACCTATAGCAACGAGTGTGAGCTACAGCGTGCACAGTGCAACCAGCAAC
GGCGCATCCGCCTACTCCGCCAAGGGCCATGTGGGTCCCGGGACCCCTGTGCCAATGTGACCTGCAGTTT
CGGTAGTACCTGTGTACCTTCAGCCGATGGACAGACCGCCTCGTGTCTGTGTCCTACAACCTGCTTTGGG
GCCCCTGATGGCACAGTGTGTGGCAGTGATGGTGTTGACTACCCTAGTGAGTGCCAGCTGCTCCGTCATG
CCTGTGCCAACCAGGAGCACATCTTTAAGAAGTTCGATGGTCCTTGTGACCCCTGCCAGGGCAGCATGTC
AGACCTGAATCATATTTGCCGGGTGAACCCACGTACACGGCACCCAGAAATGCTTCTGCGGCCTGAGAAC
TGCCCCGCCCAACACACACCTATCTGTGGAGATGATGGGGTCACCTATGAAAACGACTGTGTCATGAGCC
GTATAGGTGCAGCCCGTGGCCTGCTTCTCCAGAAAGTGCGCTCTGGTCAATGCCAGACTCGAGACCAGTG
CCCGGAGACCTGCCAGTTTAACTCTGTATGCCTGTCCCGCCGCGGCCGTCCCCACTGTTCCTGCGATCGC
GTCACCTGTGATGGGGCTTACAGGCCAGTGTGTGCCCAGGATGGGCACACGTATGACAATGACTGTTGGC
GCCAACAGGCCGAGTGTCGACAACAGCAGACCATTCCCCCCAAGCACCAGGGCCCGTGTGACCAGACCCC
ATCCCCGTGCCGTGGAGCGCAGTGTGCATTTGGGGCAACATGCACAGTGAAGAATGGGAAAGCTGTGTGC
GAGTGCCAGCGGGTGTGCTCGGGCGGCTACGATCCTGTGTGCGGCAGTGATGGTGTCACTTACGGCAGTG
TGTGCGAGCTGGAATCCATGGCCTGTACCCTTGGGCGGGAAATCCGAGTGGCCCGCAGAGGACCGTGTGA
CCGATGTGGGCAGTGCCGGTTTGGATCCTTGTGCGAGGTGGAGACTGGACGCTGTGTGTGCCCCTCTGAG
TGTGTGGAGTCAGCCCAGCCCGTATGTGGCTCTGACGGACACACATATGCTAGTGAATGTGAGCTGCATG
TCCACGCCTGTACACACCAGATCAGCCTATACGTGGCCTCAGCCGGACACTGCCAGACCTGTGGAGAAAC
AGTTTGTACCTTTGGGGCTGTGTGCTCAGCTGGACAGTGTGTATGTCCCCGTTGTGAGCACCCCCCACCT
GGCCCTGTGTGCGGCAGTGATGGCGTCACCTACCTCAGTGCCTGTGAGCTCCGAGAGGCTGCCTGTCAGC
AGCAGGTACAAATTGAGGAGGCCCGTGCAGGGCCGTGTGAGCCGGCTGAGTGTGGCTCAGGGGGCTCTGG
GTCTGGGGAAGACAATGCGTGTGAGCAGGAGCTGTGTCGGCAGCATGGTGGTGTCTGGGATGAGGACTCA
GAAGACGGGCCGTGTGTCTGTGACTTTAGTTGCCAGAGTGTCCTTAAAAGCCCAGTGTGTGGCTCAGATG
GAGTCACCTATAGCACGGAGTGCCATCTGAAGAAGGCCAGATGTGAAGCGCGGCAAGAGCTGTACGTCGC
TGCTCAGGGAGCCTGCCGGGGCCCTACCTTGGCTCCACTGCTACCTATGGCCTCCCCACACTGTGCCCAA
ACCCCCTATGGCTGCTGCCAGGACAATGTCACTGCTGCCCAGGGTGTGGGCTTGGCTGGCTGTCCCAGCA
CCTGCCATTGCAACCCACACGGCTCCTATAGCGGCACTTGTGACCCAGTCACAGGGCAGTGCTCCTGCCG
ACCAGGTGTAGGAGGCCTCAGGTGTGATCGCTGTGAGCCTGGCTTCTGGAACTTCCGTGGCATTGTCACC
GATGGACATAGTGGTTGCACTCCCTGCAGCTGTGACCCTCGGGGTGCTGTAAGAGATGACTGTGAGCAGA
TGACTGGATTGTGTTCCTGTAGACCTGGTGTGGCTGGTCCCAAGTGTGGGCAGTGTCCAGATGGTCAAGC
CCTGGGCCATCTTGGCTGTGAAGCAGATCCCACAACACCAGTGACTTGTGTGGAAATGCACTGTGAGTTT
GGCGCCTCCTGCGTAGAGGAGGCTGGTTTTGCCCAGTGTGTCTGCCCAACTCTCACATGTCCAGAGGCTA
ACTCTACCAAGGTCTGTGGATCAGATGGTGTCACATACGGCAATGAATGCCAGCTGAAGACCATTGCCTG
CCGCCAGCGTCTGGACATCTCCATTCAGAGTCTTGGTCCATGCCGGGAGAGTGTTGCTCCTGGGGTTTCC
CCTACATCTGCATCTATGACCACCCCAAGGCATATCCTGAGCAGGACACTGGCGTCTCCCCACAGCAGCC
TTCCTCTGTCTCCCAGCACTACTGCCCATGATTGGCCCACCCCATTACCCACATCACCTCAGACCGTAGT
CGGCACCCCCAGGAGCACTGCAGCCACACCCTCTGATGTGGCCAGTCTTGCTACAGCGATCTTCAGGGAA
TCTGGCAGCACCAACGGCAGTGGCGATGAGGAGCTCAGTGGCGATGAGGAGGCCAGTGGGGGCGGGTCTG
GGGGACTTGAGCCCCCGGTGGGCAGCGTTGTGGTGACCCACGGGCCACCCATCGAGAGGGCTTCCTGTTA
CAACTCACCTTTGGGCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCAGAAGGCTCCAACTGTCCA
GCTACCAAGGCATTCCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCAG
AGATGGCTGACCCCAAGTCAGAGTTGTTTGGGGAGACTGCAAGGAGCATTGAGAGCACGCTGGACGACCT
GTTCCGGAATTCGGATGTTAAGAAGGACTTCTGGAGCATCCGCCTACGGGAACTGGGGCCTGGCAAATTA
GTCCGTGCCATTGTGGATGTTCACTTTGACCCCACCACAGCCTTCCAGGCACCAGATGTGGGTCAGGCCT
TGCTCCAACAGATCCAGGTATCCAGGCCGTGGGCCCTGGCAGTGAGGAGGCCTCTGCGGGAGCATGTGCG
ATTCTTGGACTTTGACTGGTTTCCCACTTTTTTTACGGGAGCTGCAACAGGAACCACAGCTGCTGTGGCC
ACAGCCAGAGCCACCACTGTGAGCCGACTGTCTGCCTCTTCTGTCACCCCACGAGTCTACCCCAGTTACA
CCAGCCGGCCTGTTGGCAGAACTACGGCACCGCTAACCACTCGCCGGCCACCAACCACTACCGCCAGTAT
TGACCGACCTCGGACTCCAGGCCCGCAACGGCCCCCAAAGTCCTGTGATTCCCAGCCTTGCCTCCACGGA
GGTACCTGCCAGGACCTGGATTCTGGCAAGGGTTTCAGCTGCAGCTGTACTGCAGGCAGGGCTGGCACTG
TCTGTGAGAAAGTGCAGCTCCCCTCTGTGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCT
CCGAGCCTACCACACGCTGCGTCTGGCACTAGAATTCCGGGCGCTGGAGACAGAGGGACTGCTGCTCTAC
AATGGCAATGCACGTGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGTCATGTACAGTTCAGGTTCG
ACACGGGCTCAGGGCCGGCGGTGCTAACAAGCTTAGTGCCAGTGGAACCGGGACGGTGGCACCGCCTCGA
GTTGTCACGGCATTGGCGGCAGGGCACACTTTCTGTGGATGGCGAGGCTCCTGTTGTAGGTGAAAGTCCG
AGTGGCACTGATGGCCTCAACTTGGACACGAAGCTCTATGTGGGTGGTCTCCCAGAAGAACAAGTTGCCA
CGGTGCTTGATCGGACCTCTGTGGGCATCGGCCTGAAAGGATGCATTCGTATGTTGGACATCAACAACCA
GCAGCTGGAGCTGAGCGATTGGCAGAGGGCTGTGGTTCAAAGCTCTGGTGTGGGGGAATGCGGAGACCAT
CCCTGCTCACCTAACCCCTGCCATGGCGGGGCCCTCTGCCAGGCCCTGGAGGCTGGCGTGTTCCTCTGTC
AGTGCCCACCTGGCCGCTTTGGCCCAACTTGTGCAGATGAAAAGAACCCCTGCCAACCGAACCCCTGCCA
CGGGTCAGCCCCCTGCCATGTGCTTTCCAGGGGTGGGGCCAAGTGTGCGTGCCCCCTGGGACGCAGTGGT
TCCTTCTGTGAGACAGTCCTGGAGAATGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCT
ACCTGGAACTGAAAGGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTT
CTTGGCTCGTGGGCCCAGTGGCTTACTCCTCTACAATGGGCAGAAGACGGATGGCAAGGGGGACTTTGTA
TCCCTGGCCCTGCATAACCGGCACCTAGAGTTCCGCTATGACCTTGGCAAGGGGGCTGCAATCATCAGGA
GCAAAGAGCCCATAGCCCTGGGCACCTGGGTTAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCT
TCAAGTGGGTGATGGGCCCCGTGTGCTAGGGGAATCTCCGGTCCCGCACACCATGCTCAACCTCAAGGAG
CCCCTCTATGTGGGGGGAGCTCCTGACTTCAGCAAGCTGGCTCGGGGCGCTGCAGTGGCCTCCGGCTTTG
ATGGTGCCATCCAGCTGGTGTCTCTAAGAGGCCATCAGCTGCTGACTCAGGAGCATGTGTTGCGGGCAGT
AGATGTAGCGCCTTTTGCAGGCCACCCTTGTACCCAGGCCGTGGACAACCCCTGCCTTAATGGGGGCTCC
TGTATCCCGAGGGAAGCCACTTATGAGTGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAGAAGG
GGATAGTTGAGAAGTCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAGTACCT
CAATGCTGTGACTGAGAGTGAGAAAGCGCTGCAGAGCAACCACTTTGAGCTGAGCTTACGCACTGAGGCC
ACGCAGGGGCTGGTGCTGTGGATTGGAAAGGTTGGAGAACGTGCAGACTACATGGCTCTGGCCATTGTGG
ATGGGCACCTACAACTGAGCTATGACCTAGGCTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAA
CACCAACCGCTGGCTTCGAGTCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAA
GCCCCTGTGACTGGCTCTTCCCCGCTGGGTGCCACACAATTGGACACAGATGGAGCCCTGTGGCTTGGAG
GCCTACAGAAGCTTCCTGTGGGGCAGGCTCTCCCCAAGGCCTATGGCACGGGTTTTGTGGGCTGTCTGCG
GGACGTGGTAGTGGGCCATCGCCAGCTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCC
TGCCCCACTCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites AscI-MluI     
ACCN NM_021604
Insert Size 6105 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021604.3, NP_067617.3
RefSeq Size 7379 bp
RefSeq ORF 6105 bp
Locus ID 11603
Gene Summary Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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