Agrn (NM_021604) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Agrin; nmf380
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224986 representing NM_021604 Red=Cloning site Blue=ORF CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGTCCGCCCGCGGCTGAGCTTCCCGGCCCCGCTGCTGCCGCTGCTGCTGCTCCTGGCGGCGGCCGCGC CAGCCGTGCCGGGCGCCAGCGGGACGTGCCCGGAGCGCGCCTTGGAGCGGCGCGAGGAGGAGGCGAACGT GGTGCTCACCGGCACAGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACCTACTCCTGCAAGGTT CGAGTGTGGCGGTACTTGAAAGGCAAAGATGTGGTGGCCCAAGAGAGCCTGCTGGATGGTGGCAACAAGG TGGTAATTGGTGGCTTTGGAGACCCCCTCATCTGCGACAACCAGGTGTCCACCGGGGACACTAGAATCTT CTTTGTGAACCCAGCACCACCCTACCTGTGGCCAGCCCACAAGAATGAGTTGATGCTCAACTCCAGCCTT ATGCGGATCACTTTGCGGAACCTGGAAGAGGTGGAGTTCTGTGTAGAAGACAAACCTGGGATCCACTTCA CAGCAGCGCCTTCTATGCCTCCTGATGTGTGCAGGGGAATGTTATGTGGCTTTGGTGCTGTGTGTGAACC TAGTGTTGAGGATCCAGGCCGGGCCTCCTGTGTGTGCAAGAAGAATGTCTGCCCTGCTATGGTAGCTCCT GTGTGTGGCTCAGATGCTTCCACCTATAGCAACGAGTGTGAGCTACAGCGTGCACAGTGCAACCAGCAAC GGCGCATCCGCCTACTCCGCCAAGGGCCATGTGGGTCCCGGGACCCCTGTGCCAATGTGACCTGCAGTTT CGGTAGTACCTGTGTACCTTCAGCCGATGGACAGACCGCCTCGTGTCTGTGTCCTACAACCTGCTTTGGG GCCCCTGATGGCACAGTGTGTGGCAGTGATGGTGTTGACTACCCTAGTGAGTGCCAGCTGCTCCGTCATG CCTGTGCCAACCAGGAGCACATCTTTAAGAAGTTCGATGGTCCTTGTGACCCCTGCCAGGGCAGCATGTC AGACCTGAATCATATTTGCCGGGTGAACCCACGTACACGGCACCCAGAAATGCTTCTGCGGCCTGAGAAC TGCCCCGCCCAACACACACCTATCTGTGGAGATGATGGGGTCACCTATGAAAACGACTGTGTCATGAGCC GTATAGGTGCAGCCCGTGGCCTGCTTCTCCAGAAAGTGCGCTCTGGTCAATGCCAGACTCGAGACCAGTG CCCGGAGACCTGCCAGTTTAACTCTGTATGCCTGTCCCGCCGCGGCCGTCCCCACTGTTCCTGCGATCGC GTCACCTGTGATGGGGCTTACAGGCCAGTGTGTGCCCAGGATGGGCACACGTATGACAATGACTGTTGGC GCCAACAGGCCGAGTGTCGACAACAGCAGACCATTCCCCCCAAGCACCAGGGCCCGTGTGACCAGACCCC ATCCCCGTGCCGTGGAGCGCAGTGTGCATTTGGGGCAACATGCACAGTGAAGAATGGGAAAGCTGTGTGC GAGTGCCAGCGGGTGTGCTCGGGCGGCTACGATCCTGTGTGCGGCAGTGATGGTGTCACTTACGGCAGTG TGTGCGAGCTGGAATCCATGGCCTGTACCCTTGGGCGGGAAATCCGAGTGGCCCGCAGAGGACCGTGTGA CCGATGTGGGCAGTGCCGGTTTGGATCCTTGTGCGAGGTGGAGACTGGACGCTGTGTGTGCCCCTCTGAG TGTGTGGAGTCAGCCCAGCCCGTATGTGGCTCTGACGGACACACATATGCTAGTGAATGTGAGCTGCATG TCCACGCCTGTACACACCAGATCAGCCTATACGTGGCCTCAGCCGGACACTGCCAGACCTGTGGAGAAAC AGTTTGTACCTTTGGGGCTGTGTGCTCAGCTGGACAGTGTGTATGTCCCCGTTGTGAGCACCCCCCACCT GGCCCTGTGTGCGGCAGTGATGGCGTCACCTACCTCAGTGCCTGTGAGCTCCGAGAGGCTGCCTGTCAGC AGCAGGTACAAATTGAGGAGGCCCGTGCAGGGCCGTGTGAGCCGGCTGAGTGTGGCTCAGGGGGCTCTGG GTCTGGGGAAGACAATGCGTGTGAGCAGGAGCTGTGTCGGCAGCATGGTGGTGTCTGGGATGAGGACTCA GAAGACGGGCCGTGTGTCTGTGACTTTAGTTGCCAGAGTGTCCTTAAAAGCCCAGTGTGTGGCTCAGATG GAGTCACCTATAGCACGGAGTGCCATCTGAAGAAGGCCAGATGTGAAGCGCGGCAAGAGCTGTACGTCGC TGCTCAGGGAGCCTGCCGGGGCCCTACCTTGGCTCCACTGCTACCTATGGCCTCCCCACACTGTGCCCAA ACCCCCTATGGCTGCTGCCAGGACAATGTCACTGCTGCCCAGGGTGTGGGCTTGGCTGGCTGTCCCAGCA CCTGCCATTGCAACCCACACGGCTCCTATAGCGGCACTTGTGACCCAGTCACAGGGCAGTGCTCCTGCCG ACCAGGTGTAGGAGGCCTCAGGTGTGATCGCTGTGAGCCTGGCTTCTGGAACTTCCGTGGCATTGTCACC GATGGACATAGTGGTTGCACTCCCTGCAGCTGTGACCCTCGGGGTGCTGTAAGAGATGACTGTGAGCAGA TGACTGGATTGTGTTCCTGTAGACCTGGTGTGGCTGGTCCCAAGTGTGGGCAGTGTCCAGATGGTCAAGC CCTGGGCCATCTTGGCTGTGAAGCAGATCCCACAACACCAGTGACTTGTGTGGAAATGCACTGTGAGTTT GGCGCCTCCTGCGTAGAGGAGGCTGGTTTTGCCCAGTGTGTCTGCCCAACTCTCACATGTCCAGAGGCTA ACTCTACCAAGGTCTGTGGATCAGATGGTGTCACATACGGCAATGAATGCCAGCTGAAGACCATTGCCTG CCGCCAGCGTCTGGACATCTCCATTCAGAGTCTTGGTCCATGCCGGGAGAGTGTTGCTCCTGGGGTTTCC CCTACATCTGCATCTATGACCACCCCAAGGCATATCCTGAGCAGGACACTGGCGTCTCCCCACAGCAGCC TTCCTCTGTCTCCCAGCACTACTGCCCATGATTGGCCCACCCCATTACCCACATCACCTCAGACCGTAGT CGGCACCCCCAGGAGCACTGCAGCCACACCCTCTGATGTGGCCAGTCTTGCTACAGCGATCTTCAGGGAA TCTGGCAGCACCAACGGCAGTGGCGATGAGGAGCTCAGTGGCGATGAGGAGGCCAGTGGGGGCGGGTCTG GGGGACTTGAGCCCCCGGTGGGCAGCGTTGTGGTGACCCACGGGCCACCCATCGAGAGGGCTTCCTGTTA CAACTCACCTTTGGGCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCAGAAGGCTCCAACTGTCCA GCTACCAAGGCATTCCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCAG AGATGGCTGACCCCAAGTCAGAGTTGTTTGGGGAGACTGCAAGGAGCATTGAGAGCACGCTGGACGACCT GTTCCGGAATTCGGATGTTAAGAAGGACTTCTGGAGCATCCGCCTACGGGAACTGGGGCCTGGCAAATTA GTCCGTGCCATTGTGGATGTTCACTTTGACCCCACCACAGCCTTCCAGGCACCAGATGTGGGTCAGGCCT TGCTCCAACAGATCCAGGTATCCAGGCCGTGGGCCCTGGCAGTGAGGAGGCCTCTGCGGGAGCATGTGCG ATTCTTGGACTTTGACTGGTTTCCCACTTTTTTTACGGGAGCTGCAACAGGAACCACAGCTGCTGTGGCC ACAGCCAGAGCCACCACTGTGAGCCGACTGTCTGCCTCTTCTGTCACCCCACGAGTCTACCCCAGTTACA CCAGCCGGCCTGTTGGCAGAACTACGGCACCGCTAACCACTCGCCGGCCACCAACCACTACCGCCAGTAT TGACCGACCTCGGACTCCAGGCCCGCAACGGCCCCCAAAGTCCTGTGATTCCCAGCCTTGCCTCCACGGA GGTACCTGCCAGGACCTGGATTCTGGCAAGGGTTTCAGCTGCAGCTGTACTGCAGGCAGGGCTGGCACTG TCTGTGAGAAAGTGCAGCTCCCCTCTGTGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCT CCGAGCCTACCACACGCTGCGTCTGGCACTAGAATTCCGGGCGCTGGAGACAGAGGGACTGCTGCTCTAC AATGGCAATGCACGTGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGTCATGTACAGTTCAGGTTCG ACACGGGCTCAGGGCCGGCGGTGCTAACAAGCTTAGTGCCAGTGGAACCGGGACGGTGGCACCGCCTCGA GTTGTCACGGCATTGGCGGCAGGGCACACTTTCTGTGGATGGCGAGGCTCCTGTTGTAGGTGAAAGTCCG AGTGGCACTGATGGCCTCAACTTGGACACGAAGCTCTATGTGGGTGGTCTCCCAGAAGAACAAGTTGCCA CGGTGCTTGATCGGACCTCTGTGGGCATCGGCCTGAAAGGATGCATTCGTATGTTGGACATCAACAACCA GCAGCTGGAGCTGAGCGATTGGCAGAGGGCTGTGGTTCAAAGCTCTGGTGTGGGGGAATGCGGAGACCAT CCCTGCTCACCTAACCCCTGCCATGGCGGGGCCCTCTGCCAGGCCCTGGAGGCTGGCGTGTTCCTCTGTC AGTGCCCACCTGGCCGCTTTGGCCCAACTTGTGCAGATGAAAAGAACCCCTGCCAACCGAACCCCTGCCA CGGGTCAGCCCCCTGCCATGTGCTTTCCAGGGGTGGGGCCAAGTGTGCGTGCCCCCTGGGACGCAGTGGT TCCTTCTGTGAGACAGTCCTGGAGAATGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCT ACCTGGAACTGAAAGGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTT CTTGGCTCGTGGGCCCAGTGGCTTACTCCTCTACAATGGGCAGAAGACGGATGGCAAGGGGGACTTTGTA TCCCTGGCCCTGCATAACCGGCACCTAGAGTTCCGCTATGACCTTGGCAAGGGGGCTGCAATCATCAGGA GCAAAGAGCCCATAGCCCTGGGCACCTGGGTTAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCT TCAAGTGGGTGATGGGCCCCGTGTGCTAGGGGAATCTCCGGTCCCGCACACCATGCTCAACCTCAAGGAG CCCCTCTATGTGGGGGGAGCTCCTGACTTCAGCAAGCTGGCTCGGGGCGCTGCAGTGGCCTCCGGCTTTG ATGGTGCCATCCAGCTGGTGTCTCTAAGAGGCCATCAGCTGCTGACTCAGGAGCATGTGTTGCGGGCAGT AGATGTAGCGCCTTTTGCAGGCCACCCTTGTACCCAGGCCGTGGACAACCCCTGCCTTAATGGGGGCTCC TGTATCCCGAGGGAAGCCACTTATGAGTGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAGAAGG GGATAGTTGAGAAGTCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAGTACCT CAATGCTGTGACTGAGAGTGAGAAAGCGCTGCAGAGCAACCACTTTGAGCTGAGCTTACGCACTGAGGCC ACGCAGGGGCTGGTGCTGTGGATTGGAAAGGTTGGAGAACGTGCAGACTACATGGCTCTGGCCATTGTGG ATGGGCACCTACAACTGAGCTATGACCTAGGCTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAA CACCAACCGCTGGCTTCGAGTCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAA GCCCCTGTGACTGGCTCTTCCCCGCTGGGTGCCACACAATTGGACACAGATGGAGCCCTGTGGCTTGGAG GCCTACAGAAGCTTCCTGTGGGGCAGGCTCTCCCCAAGGCCTATGGCACGGGTTTTGTGGGCTGTCTGCG GGACGTGGTAGTGGGCCATCGCCAGCTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCC TGCCCCACTCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_021604
Insert Size	6105 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021604.3, NP_067617.3
RefSeq Size	7379 bp
RefSeq ORF	6105 bp
Locus ID	11603
Gene Summary	Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.[UniProtKB/Swiss-Prot Function]

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