Rhot1 (NM_001163354) Mouse Tagged ORF Clone
CAT#: MG224107
- TrueORF®
Rhot1 (tGFP-tagged) - Mouse ras homolog gene family member T1 (Rhot1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001163354" in other vectors (3)
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CNY 6,536.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2210403N23Rik; AA415293; AF244542; AI834919; Arht1; C430039G08Rik; Miro1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224107 representing NM_001163354
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGCGGGCCGAGTGCGGCCCCTGCGAGCCTCCGACATGAAGAAAGACGTGAGGATCCTGCTGGTGG GAGAACCTCGAGTTGGGAAGACATCACTGATTATGTCGCTGGTCAGTGAAGAATTTCCGGAAGAGGTTCC TCCCCGAGCAGAAGAAATCACCATTCCAGCTGACGTCACCCCTGAAAGAGTTCCAACACACATTGTCGAT TATTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCACAGGCTAATGTTATCTGCATAG TTTATGCTGTTAACAACAAACATTCTATTGATAAGGTAACAAGCCGATGGATTCCTCTCATAAATGAAAG AACAGACAAAGACAGCAGGCTGCCTTTGATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGT ATGGAGACCATCCTTCCAATTATGAACCAGTATACAGAGATAGAGACCTGTGTGGAGTGTTCAGCAAAAA ACCTAAAGAATATCTCAGAGCTGTTTTATTATGCACAGAAAGCTGTTCTTCATCCTACAGGGCCTCTATA CTGCCCAGAGGAGAAAGAGATGAAACCGGCTTGTATAAAGGCCCTCACTCGTATATTTAAAATATCTGAT CAGGATAACGACGGCACTCTGAATGATGCTGAGCTCAACTTCTTCCAGAGAATTTGTTTCAACACTCCTT TAGCTCCGCAAGCGCTGGAAGATGTCAAGAATGTGGTTAGAAAGCACTTAAGTGACGGTGTGGCTGACAG TGGGCTGACGCTCAGAGGTTTTCTCTTTTTACATACACTTTTTATCCAGAGGGGGAGGCATGAGACTACT TGGACTGTGCTTCGACGATTTGGTTATGACGATGACCTGGACCTGACGCCTGAGTATTTATTCCCCCTGC TAAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTGTTTCTCCAGAGCACCTTTGA CAAACATGATTTGGACAGAGACTGTGCTTTGTCGCCTGATGAACTTAAAGATTTATTTCAAGTTTTTCCT TACATACCTTGGGGACCAGATGTAAATAACACAGTTTGCACAAATGAAAGAGGCTGGATAACCTACCAGG GGTTCCTCTCCCAGTGGACGCTCACCACCTACTTAGATGTGCAGCGTTGCCTGGAGTATTTGGGATATCT AGGCTATTCCATACTGACGGAACAAGAGTCTCAAGCTTCAGCCATTACAGTAACAAGGGATAAAAAGATT GACCTGCAGAAAAAACAGACTCAGAGAAATGTGTTCAGGTGTAATGTAATTGGAGTGAAAGGCTGTGGGA AAACTGGAGTTCTTCAGTCTCTCCTTGGAAGAAACCTAATGAGGCAGAAGAAAATTCGTGATGATCATAA GTCCTATTATGCAATTAACACTGTCTATGTATATGGACAAGAGAAATACTTGTTGCTGCACGATATCTCA GAGTCGGAATTTCTAACTGAGGCTGAGACCATTTGTGATGTTGTATGCCTGGTATATGATGTCACCAATC CCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACATTTTATGGACAGCAGAATTCCCTGCTTGAT TGTAGCTGCAAAGTCAGACCTGCATGAAGTCAAACAGGAACATAGCATCTCACCTACTGATTTCTGCAGG AAACACAAAATGCCTCCACCTCAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTG TTAAGTTGACAACAATGGCCATGTACCCAGAGGATCATTACAGAGGCAGCCTCTCCCGAGACATGGGCAG CACTGATAGAATAGAGAATTTGAGAAAAATCTGGGTCTTTCTAAAAACTGCTTTGCACGTGACACAAGCT GACCTCAAGAGCTCAACGTTCTGGCTTCGCGCAAGCTTTGGTGCTACGGTCTTTGCAGTCGTGGGCTTTG CTATGTACAGAGCACTACTGAAACAGCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224107 representing NM_001163354
Red=Cloning site Green=Tags(s) MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVD YSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSS METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISD QDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETT WTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFP YIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKI DLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDIS ESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCR KHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTALHVTQA DLKSSTFWLRASFGATVFAVVGFAMYRALLKQR TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163354 |
ORF Size | 1989 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163354.2 |
RefSeq Size | 4233 bp |
RefSeq ORF | 1992 bp |
Locus ID | 59040 |
UniProt ID | Q8BG51 |
Gene Summary | Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin
,Boukelmoune, N;Chiu, GS;Kavelaars, A;Heijnen, CJ;,
Acta Neuropathol Commun
,PubMed ID 30541620
[RHOT1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224107 | Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 2 |
CNY 4,936.00 |
|
MR224107L3 | Lenti ORF clone of Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 2 |
CNY 7,700.00 |
|
MR224107L4 | Lenti ORF clone of Rhot1 (mGFP-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 2 |
CNY 7,336.00 |