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Smg7 (NM_001160256) Mouse Tagged ORF Clone
CAT＃: MG223777
- TrueORF®
Smg7 (tGFP-tagged) - Mouse Smg-7 homolog nonsense mediated mRNA decay factor (C. elegans) (Smg7) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001160256" in other vectors (3)
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CNY 12,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9430023P16Rik; mKIAA0250 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223777 representing NM_001160256
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGCAGAGCGCGCAGTATCTCCGGCAGGCAGAAGTCCTGAAGGCTGAAATGACAGATTCTAAGC TGGGTCCGGCTGAGGTCTGGACATCCAGGCAAGCTCTGCAGGACTTGTACCAGAAAATGCTAGTTACTGA TCTGGAATACGCTTTAGACAAGAAAGTAGAACAGGATCTCTGGAATCATGCCTTTAAGAATCAGATCACA ACACTGCAAGGCCAAGCCAAGAATCGAGCAAACCCAAATCGAAGTGAAGTCCAGGCAAACCTTTCTCTGT TCCTAGAGGCAGCTAGCGGCTTCTATACTCAGTTATTACAGGAACTGTGTACGGTGTTTAATGTAGATTT GCCATGCCGTGTGAAGTCTTCCCAGTTGGGAATTATTAGCAATAAACAGACGCACAGCAGCACCATAGTG AAGCCACAGTCTAGCTCCTGCTCCTACATTTGCCAACACTGCCTCGTCCACCTTGGAGACATTGCTCGAT ACAGAAATCAGACTAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAGCTTGTCCCCTCCAATGGTCA GCCTTACAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCACCTGACCACAATTTTCTACTACTGC AGAAGCATTGCTGTGAAATTCCCTTTCCCAGCTGCCTCCACTAATCTACAAAAAGCACTTTCTAAAGCAC TGGAAAGCCGGGATGAGTTGAAAACCAAGTGGGGTGTTTCTGACTTCATCAAGGCCTTTATTAAATTCCA CGGTCATGTATACCTGAGTAAGAGTTTGGAAAAGCTGAGCCCTCTTCGAGAGAAGTTGGAAGAACAGTTT AAGAGACTGCTGTTCCAAAAGGCTTTCAACTCTCAACAGTTAGTTCATGTCACTGTCATTAACCTGTTTC AACTTCATCATCTTCGTGACTTTAGCAATGAAACAGAGCAACACAGTTACAGCCAAGATGAACAGCTGTG TTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTCGGCATCCTGTGCAAATGTCCTCTCCAGAATGAT TCTCAGGAGTCCAACAATGCCTACCCCCTTCCTGCAGTCAAGGTCTCCATGGACTGGCTAAGACTCCGAC CTAGAGTCTTTCAAGAAGCAGTGGTGGATGAAAGACAGTACATTTGGCCCTGGCTAATTTCTCTTCTAAA TAGTTTCCATCCCCGTGAAGATGATCTCTCAAATACTAATGCCACACCACTTCCAGAGGAGTTTGAATTA CAAGGATTCTTGGCTTTAAGACCTTCTTTCAGGAACTTGGATTTTTCCAAAGGCCATCAGGGTATTACAG GAGACAAAGAGGGTCAACAACGACGAATACGACAGCAGCGTTTGATCTCCATAGGGAAATGGATTGCTGA TAACCAGCCACGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTGTTATTTATCACAGAAATCCCAGAA TTAATACTAGAAGACCCCAGTGAAGCCAAAGAGAACCTCATCCTACAAGAAACATCTGTGGTAGAGTCAC TGGCTACGGATGGGAGCCCAGGACTGAAATCAGTGCTGTCTACAGGCCGAAATCCAAGCAACAGCTGTGA CTCAGGAGAGAAACCAGTGGTCACCTTTAAAGAGAACATTAAGCCACGAGAAGTGAACCAAGGAAGAAGC TTTCCTCCCAAAGAGGTAAAATCCCAGACAGAACTAAGAAAGACTCCAGTGTCTGAAGCCAGGAAAACGC CTGTCACTCAAACTCCAAGTCAAACGAGTAATTCTCAGTTCATCCCCATCCATCACCCTGGAGCCTTCCC TCCTCTTCCCAGCCGACCAGGATTCCCGCCCCCAACATATGTTATCCCCCCTCCTGTGGCATTTTCTATG GGCTCAGGTTACACCTTCCCAGCTGGTGTTTCTGTCCCAGGAACCTTTCTTCAGTCTACAGCTCACTCTC CAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACATTCCTTACAGCCAGCAACGGCCCTCTGGACC AGGGCCAATGAACCAGGGACCTCAACAGTCACAGCCACCTTCCCAGCCACCCCTTACATCTTTACCAGCT CAGCCAACAGCACAGTCTACAAGCCAATTGCAGGTTCAAGCTCTAGCTCAGCAACAGCAATCCCCTACAA AAGTCATACCAGCTTTGGGGAAAAGCCCGCCTCACCACTCTGGATTCCAGCAGTATCAACAGGCAGATGC CTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAAGCCCACTAGGGAAAATTATGCCTGTGAAACAGTCC TACTACCTTCAGACCCAAGACCCTATAAAACTGTTTGAGCCGTCATTGCAACCTCCTGTAATACAGCAGC AGCCTCTAGAGAAAAAAATGAAGCCTTTCCCCATGGAGCCATATAACCATAATCCCTCAGAAGTCAAGGT CCCAGAGTTCTACTGGGATTCTTCCTACAGCATGGCCGATAACAGAGCAGTAATGGCTCAGCAACCAAAT ATGGACCGCAGGAGCAAACGGTCACCTGGAGTCTTCCGTCCAGAACAGGATCCTGTGCCCAGGATGCCAT TTGAGGACCCCAAGAGCTCCCCTCTGCTTCCTCCGGACCTGTTAAAGAGTCTGGCTGCCTTGGAGGAAGA GGAAGAGCTGATCTTTTCTAACCCTCCTGATCTTTACCCAGCTCTGCTGGGTCCTCTCGCCTCTCTTCCT GGACGAAGCCTCTTTAAATCCTTATTGGAGAAGCCTTCTGAGCTCATGTCACATTCATCTTCTTTCCTGT CCCTCACGGGGTTCTCTGTCAATCAGGAAAGATATCCAAACAGCAGTATGTTCAATGAAGTGTATGGAAA AAACCTGACAACCAGCTCCAAGGCAGAACTGAACCCCTCAGTCGCCTCCCAGGAAACATCACTGTACTCC CTTTTTGAAGGGACCCCATGGTCTCCATCACTTCCTGCCAGTTCAGATCATTCGACACCAGCCAGCCAGT CTCCTCATTCCTCTAACCCAAGCAGCCTGCCCAGTTCTCCTCCAACGCATAACCATAATTCTGCTCCATT CTCTAATTTTGGACCCATTGGGACTCCAGATAACAGGGATAGGCGGCCTGCAGATAGGTGGAAAACTGAT AAACCAGCCATGGGTGGTTTTGGTGTTGATTATCTCTCAGCAACGTCATCATCTGAGAGCAGTTGGCATC AGGCTAGCACTCCGAGTGGCACCTGGACAGGCCACGGCCCCTCCATGGAGGATTCCTCTGCTGTCCTCAT GGAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATGCATCCTGGGCCCTCCGCTCTGGAGCAGTTGTTA ATGCAGCAGAAGCAGAAACAGCAGCGGGGACAAGGTGCCATGAACCCTCCACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223777 representing NM_001160256
Red=Cloning site Green=Tags(s) MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQIT TLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIV KPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYC RSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQF KRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQND SQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFEL QGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPE LILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRS FPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSM GSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPA QPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQS YYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPN MDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLP GRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYS LFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTD KPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLL MQQKQKQQRGQGAMNPPH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160256 |
| ORF Size | 3414 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001160256.1, NP_001153728.1 |
| RefSeq Size | 5867 bp |
| RefSeq ORF | 3417 bp |
| Locus ID | 226517 |
| UniProt ID | Q5RJH6 |
| Gene Summary | Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR223777 | Smg7 (Myc-DDK-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2 |
CNY 11,310.00 |
|
| MR223777L3 | Lenti ORF clone of Smg7 (Myc-DDK-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2 |
CNY 13,210.00 |
|
| MR223777L4 | Lenti ORF clone of Smg7 (mGFP-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2 |
CNY 13,210.00 |
