Ntn1 (NM_008744) Mouse Tagged ORF Clone
CAT#: MG223704
- TrueORF®
Ntn1 (tGFP-tagged) - Mouse netrin 1 (Ntn1), (10ug)
ORF Plasmid: DDK
"NM_008744" in other vectors (4)
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CNY 8,344.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI561871; BC019633; Netrin-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008744 |
ORF Size | 1812 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008744.2, NP_032770.2 |
RefSeq Size | 5757 bp |
RefSeq ORF | 1815 bp |
Locus ID | 18208 |
UniProt ID | O09118 |
Gene Summary | Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Bridging the Gap: The Importance of TUBA1A α-Tubulin in Forming Midline Commissures
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Frontiers in Cell and Developmental Biology
,PubMed ID 35127710
[Ntn1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219613 | Ntn1 (untagged) - Mouse netrin 1 (Ntn1), (10ug) |
CNY 6,752.00 |
|
MR223704 | Ntn1 (Myc-DDK-tagged) - Mouse netrin 1 (Ntn1) |
CNY 6,744.00 |
|
MR223704L3 | Lenti ORF clone of Ntn1 (Myc-DDK-tagged) - Mouse netrin 1 (Ntn1) |
CNY 7,890.00 |
|
MR223704L4 | Lenti ORF clone of Ntn1 (mGFP-tagged) - Mouse netrin 1 (Ntn1) |
CNY 9,144.00 |