United States
Germany
Japan
United Kingdom
China
Ntn1 (NM_008744) Mouse Untagged Clone
CAT＃: MC219613
Ntn1 (untagged) - Mouse netrin 1 (Ntn1), (10ug)
CNY 6,752.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI561871; BC019633; Netrin-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219613 representing NM_008744
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCGCGCTGTGTGGGAGGCGCTGGCGGCGCTGGCGGCGGTGGCGTGCCTGGTGGGCGCGGTGCGCG GCGGGCCCGGGCTTAGCATGTTCGCCGGCCAGGCGGCGCAGCCTGATCCTTGCTCGGATGAGAATGGACA CCCGCGCCGCTGCATCCCGGACTTTGTCAACGCGGCCTTCGGCAAGGACGTGCGCGTGTCCAGCACCTGC GGCCGGCCCCCGGCGCGCTACTGCGTGGTGAGCGAGCGTGGTGAAGAGCGGCTGCGCTCCTGTCACCTCT GCAACTCTTCGGATCCCAAGAAAGCGCACCCGCCCGCCTTCCTCACCGACCTCAATAACCCGCACAACCT GACGTGCTGGCAGTCCGAGAACTACCTGCAGTTCCCGCACAACGTGACGCTCACTCTGTCGCTCGGCAAG AAGTTTGAGGTGACCTATGTGAGCCTGCAATTCTGCTCGCCGCGGCCAGAGTCCATGGCCATCTACAAGT CCATGGACTACGGGCGCACGTGGGTGCCCTTCCAGTTCTATTCCACGCAGTGCCGCAAAATGTACAACCG GCCGCACCGCGCGCCTATCACCAAACAGAACGAGCAGGAGGCCGTGTGCACCGACTCGCACACCGACATG CGCCCGCTCTCTGGCGGGCTGATCGCTTTCAGCACGCTGGACGGGCGGCCCTCGGCGCACGACTTCGACA ACTCGCCGGTGCTGCAGGACTGGGTCACGGCCACCGACATCCGCGTGGCTTTCAGCCGCCTGCACACGTT CGGCGACGAGAACGAAGACGACTCGGAGCTGGCGCGCGACTCCTATTACTATGCAGTGTCTGACCTGCAG GTTGGCGGCCGCTGCAAGTGCAACGGCCACGCGGCGCGTTGCGTGCGCGACCGAGACGACAGTCTGGTGT GTGACTGTAGGCACAACACGGCCGGCCCTGAATGCGACCGTTGCAAGCCCTTCCACTACGACCGGCCCTG GCAGCGCGCCACGGCCCGCGAGGCCAACGAGTGCGTGGCCTGCAACTGCAACCTCCATGCTCGGCGCTGC AGATTCAACATGGAGCTCTATAAGCTATCAGGGCGCAAGAGCGGGGGAGTCTGTCTCAACTGCCGCCACA ACACTGCGGGCCGCCACTGCCACTACTGCAAGGAGGGCTTCTACCGAGACATGGGCAAGCCTATCACCCA CCGGAAGGCTTGCAAAGCCTGTGATTGCCACCCAGTGGGTGCTGCTGGCAAGACCTGCAATCAAACCACT GGCCAATGTCCCTGCAAGGACGGCGTGACGGGCATCACCTGCAACCGATGTGCCAAAGGCTACCAGCAGA GCCGCTCCCCCATCGCCCCTTGCATCAAGATTCCTGTGGCGCCACCCACCACTGCAGCCAGCAGCGTGGA GGAACCGGAAGACTGTGACTCCTATTGCAAGGCCTCCAAAGGCAAGCTGAAGATGAACATGAAGAAATAC TGCAGGAAGGACTATGCTGTCCAGATCCACATCCTGAAGGCCGACAAAGCAGGGGACTGGTGGAAGTTCA CCGTGAACATCATCTCCGTGTACAAGCAGGGCACAAGTCGTATTCGCCGTGGTGACCAGAGTTTGTGGAT CCGCTCACGAGACATCGCCTGCAAGTGTCCCAAAATCAAGCCCCTCAAGAAGTACTTGCTGTTGGGTAAT GCCGAGGACTCACCTGACCAGAGTGGCATCGTGGCAGACAAGAGCAGCCTGGTGATCCAGTGGCGGGACA CATGGGCACGGCGGCTGCGCAAGTTCCAGCAACGGGAGAAGAAGGGCAAGTGCAAGAAGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008744 |
| Insert Size | 1815 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008744.2, NP_032770.2 |
| RefSeq Size | 5757 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 18208 |
| UniProt ID | O09118 |
| Gene Summary | Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223704 | Ntn1 (tGFP-tagged) - Mouse netrin 1 (Ntn1), (10ug) |
CNY 8,344.00 |
|
| MR223704 | Ntn1 (Myc-DDK-tagged) - Mouse netrin 1 (Ntn1) |
CNY 6,744.00 |
|
| MR223704L3 | Lenti ORF clone of Ntn1 (Myc-DDK-tagged) - Mouse netrin 1 (Ntn1) |
CNY 7,890.00 |
|
| MR223704L4 | Lenti ORF clone of Ntn1 (mGFP-tagged) - Mouse netrin 1 (Ntn1) |
CNY 9,144.00 |
