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Ano3 (NM_001128103) Mouse Tagged ORF Clone
CAT＃: MG223701
- TrueORF®
Ano3 (tGFP-tagged) - Mouse anoctamin 3 (Ano3) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001128103" in other vectors (4)
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CNY 10,740.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI838058; B230324K02Rik; Tmem16c |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223701 representing NM_001128103
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCACCACTCAGGCTCCATTCAGTCCTTTAAACAGCAAAAAGGTATGAATATAAGCAAAAGTGAGA TAACAACTGAAGCGTCATTAAAACCATCTAGGAGATCCCTGCCTTGCCTGGCCCAGAGCTATGCTCATTC AAAAAGCTTGAGTCAATCTGCTTCACTGTTTCAGTCAACTGAGAGTGAATCTCAGGCTCCAACTTCTGTA ACATTTCTTTCTGCTGATAAGCCTGAACATGTTACTTCGGAGGAGAGTAGAAAGGACTCTACCCTTAAGT GTTCCTTTGCTGACCTCAGTGACTTTTGCTTGGCTCTGGGGAAAGACAAGGATTACCTGGATGAATCTGA ACATGCCAACTATGATCGATCTCGTCTCCTTAATGACTTTGTTACCAAAGATAAGCCTGCATCCAAGACA AAGCTGTCTAAGAATGACATGAGTTACATAGCATCTAGTGGACTCTTATTCAAAGATGGCAAAAAGCGAA TTGATTACATCCTGGTTTATCGAAAAACAAATATACAATATGATAAAAGAAACACATTTGAAAAGAACCT GAGAGCAGAAGGCTTGATGTTGGAGAAGGAGCCAGCAATTGCCAACCCCGACATCATGTTTATTAAAATT CACATTCCATGGGACACACTGTGCAAGTATGCAGAGAGGCTGAACATCAGGGTGCCCTTCAGGAAGAAAT GTTACTATACTGACCAGAAGAACAAATCAAAGAGCAGGGTACAAAACTATTTCAAAAGAATCAAAAAATG GATGTCCCAAAACCCAATGGTCCTTGACAAGTCAGCGTTTCCAGAGCTGGAGGAGTCGGACTGCTATACT GGACCCTTCAGCAGAGCTAGGATTCACCACTTCATAATAAACAATAAGGACACCTTCTTCAGCAATGCTA CTCGAAGCAGGATAGTTTATCACATGCTGGAACGCACCAAATATGAAAATGGAATATCAAAAGTGGGTAT CCGTAAGCTTATCACTAATGGTTCATACATAGCAGCATTTCCCCCACATGAGGGAGCCTACAAGAGTAGC CTTCCGATCAAAACCCATGGACCTCAGAATAACAGGCATCTGTTGTATGAGCGCTGGGCACGCTGGGGAA TGTGGTACAAGCATCAGCCTCTGGACTTAATCAGGATGTACTTTGGTGAGAAGATTGGCTTATACTTCGC CTGGCTGGGATGGTACACCGGAATGCTGATTCCTGCTGCAGTTGTCGGCCTGTGTGTTTTCTTCTATGGA TTAGTCACAATGAACGAAAGCCAAGTAAGCCAAGAAATCTGTAAAGCCACTGAAGTCTTCATGTGTCCTC TATGTGACAAGAATTGCTCACTGCAGAGACTCAACGACAGCTGCATCTATGCCAAGGTAACATATTTGTT TGATAATGGAGGGACAGTTTTCTTCGCTATTTTTATGGCAATATGGGCCACAGTCTTTCTTGAGTTTTGG AAAAGGAGAAGAAGCATTCTGACTTACACTTGGGACCTTATTGAATGGGAAGAAGAGGAGGAAACACTTC GCCCCCAGTTTGAAGCAAAATATTACAGGATGGAGGTAATAAACCCCATCACGGGGAAGCCTGAGCCTCA TCAGCCTTCATCCGACAAAGTCACGCGACTTCTTGTGTCTGTCTCAGGAATCTTCTTCATGATCTCCTTG GTCATCACAGCAGTGTTCGCAGTTGTAGTGTACCGTCTGGTAGTCATGGAACAGTTTGCATCATTCAAGT GGAATTTCGTCAAACAGCACTGGCAGTTTGCTACATCTGGTGCTGCTGTCTGTATCAATTTCATAATCAT CATGCTGCTGAATCTTGCTTATGAAAAAATTGCATACCTCCTCACTAACTTAGAATATCCTCGAACTGAA TCAGAATGGGAAAACAGCTTTGCCTTGAAGATGTTCCTTTTCCAGTTTGTCAATTTGAACAGTTCTATCT TCTATATTGCATTCTTTTTGGGAAGATTCGTAGGCCACCCAGGAAAATACAATAAACTTTTTGAGCGGTG GAGACTGGAGGAATGCCATCCTAGTGGCTGTCTGATAGACCTCTGCCTCCAGATGGGAGTCATCATGTTT TTGAAGCAAATATGGAACAACTTCATGGAGCTGGGATATCCGTTAATCCAAAACTGGTGGTCACGACACA AAATCAAAAGGGGAATACAAGACGCTTCCATACCACAATGGGAAAATGACTGGAACCTTCAGCCTATGAA CATCCATGGGCTGATGGACGAGTACTTAGAAATGGTTTTGCAATTTGGATTCACTACCATCTTTGTTGCT GCTTTTCCCCTGGCCCCTCTATTGGCTTTGTTAAACAATATCATAGAAATCAGATTGGATGCATATAAAT TTGTGACCCAGTGGAGGAGGCCTCTGCCAGCCCGAGCCACTGACATAGGTATCTGGCTTGGAATTCTTGA GGGAATTGGCATATTGGCTGTGATCACCAATGCATTTGTAATTGCTATTACATCTGATTACATCCCACGT TTTGTCTATGAATACAAATATGGCCCTTGTGCAAATCATGTGAAGCAGAATGAAAATTGCCTAAAGGGCT ATGTCAACAATAGCTTATCCTTCTTTGACCTGAGTGAACTTGGTATGGGAAAATCTGGTTACTGCAGGTA CCGAGACTATAGAGGCCCACCTTGGAGTTCCAAACCCTATGAATTCACCTTACAGTACTGGCACATCCTG GCTGCTCGATTGGCCTTCATTATCGTGTTTGAGCATCTTGTTTTTGGCATTAAGTCATTCATTGCATACC TGATTCCTGATATACCAAAGGGCCTCCGTGAGCGCATACGGCGAGAGAAATACTTGGTCCAGGAAATGAT GTATGAGGCTGAACTGGAACATTTACAACAACAGAGAAGAAAAAGTGGTCAGCCTATTCACCATGAATGG CCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223701 representing NM_001128103
Red=Cloning site Green=Tags(s) MVHHSGSIQSFKQQKGMNISKSEITTEASLKPSRRSLPCLAQSYAHSKSLSQSASLFQSTESESQAPTSV TFLSADKPEHVTSEESRKDSTLKCSFADLSDFCLALGKDKDYLDESEHANYDRSRLLNDFVTKDKPASKT KLSKNDMSYIASSGLLFKDGKKRIDYILVYRKTNIQYDKRNTFEKNLRAEGLMLEKEPAIANPDIMFIKI HIPWDTLCKYAERLNIRVPFRKKCYYTDQKNKSKSRVQNYFKRIKKWMSQNPMVLDKSAFPELEESDCYT GPFSRARIHHFIINNKDTFFSNATRSRIVYHMLERTKYENGISKVGIRKLITNGSYIAAFPPHEGAYKSS LPIKTHGPQNNRHLLYERWARWGMWYKHQPLDLIRMYFGEKIGLYFAWLGWYTGMLIPAAVVGLCVFFYG LVTMNESQVSQEICKATEVFMCPLCDKNCSLQRLNDSCIYAKVTYLFDNGGTVFFAIFMAIWATVFLEFW KRRRSILTYTWDLIEWEEEEETLRPQFEAKYYRMEVINPITGKPEPHQPSSDKVTRLLVSVSGIFFMISL VITAVFAVVVYRLVVMEQFASFKWNFVKQHWQFATSGAAVCINFIIIMLLNLAYEKIAYLLTNLEYPRTE SEWENSFALKMFLFQFVNLNSSIFYIAFFLGRFVGHPGKYNKLFERWRLEECHPSGCLIDLCLQMGVIMF LKQIWNNFMELGYPLIQNWWSRHKIKRGIQDASIPQWENDWNLQPMNIHGLMDEYLEMVLQFGFTTIFVA AFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLPARATDIGIWLGILEGIGILAVITNAFVIAITSDYIPR FVYEYKYGPCANHVKQNENCLKGYVNNSLSFFDLSELGMGKSGYCRYRDYRGPPWSSKPYEFTLQYWHIL AARLAFIIVFEHLVFGIKSFIAYLIPDIPKGLRERIRREKYLVQEMMYEAELEHLQQQRRKSGQPIHHEW P TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128103 |
| ORF Size | 2943 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128103.2, NP_001121575.1 |
| RefSeq Size | 2946 bp |
| RefSeq ORF | 2946 bp |
| Locus ID | 228432 |
| UniProt ID | A2AHL1 |
| Gene Summary | Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylcholine and galactosylceramide (PubMed:23532839). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:23532839). Seems to act as potassium channel regulator and may inhibit pain signaling; can facilitate KCNT1/Slack channel activity by promoting its full single-channel conductance at very low sodium concentrations and by increasing its sodium sensitivity (PubMed:23872594).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222970 | Ano3 (untagged) - Mouse anoctamin 3 (Ano3), transcript variant 2, (10ug) |
CNY 12,640.00 |
|
| MR223701 | Ano3 (Myc-DDK-tagged) - Mouse anoctamin 3 (Ano3), transcript variant 2 |
CNY 7,904.00 |
|
| MR223701L3 | Lenti ORF clone of Ano3 (Myc-DDK-tagged) - Mouse anoctamin 3 (Ano3), transcript variant 2 |
CNY 11,690.00 |
|
| MR223701L4 | Lenti ORF clone of Ano3 (mGFP-tagged) - Mouse anoctamin 3 (Ano3), transcript variant 2 |
CNY 11,690.00 |
