Gtf2a1 (NM_175335) Mouse Tagged ORF Clone
CAT#: MG223402
- TrueORF®
Gtf2a1 (tGFP-tagged) - Mouse general transcription factor II A 1 (Gtf2a1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_175335" in other vectors (4)
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CNY 4,180.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6330549H03Rik; AA536742; AA959775; AW060250; Tfiia1; TfIIAa/b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223402 representing NM_175335
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACTCGGCAAACACAAACACCGTGCCTAAATTGTATAGATCTGTGATTGAAGATGTTATTAATG ACGTACGAGACATTTTTCTGGATGACGGAGTGGATGAACAAGTCCTGATGGAACTAAAAACTTTGTGGGA GAATAAACTAATGCAGTCTAGGGCAGTAGATGGATTTCATTCAGAAGAACAGCAGCTTTTGTTGCAAGTT CAGCAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCACCACCACCAGCACCAGCAAGCGCAACCTC AGCAAACGGTACCTCAGCAAGCACAGACCCAGCAGGTCCTCATTCCTGCATCCCAGCAAGCTACAGCACC ACAAGTTATTGTTCCTGATTCTAAGTTGCTACAACATATGAATGCATCAAGTATTACGAGTGCTGCTGCT ACAGCTGCTACCTTGGCACTGCCTGCAGGAGTGACTCCGGTTCAACAGCTACTAACAAATTCAGGCCAGC TGCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAGTATATCTTGCAGCCTCAGCAGTCAGTTGTTCTACA ACAACAAGTTATACCACAAATGCAGCCTGGTGGAGTCCAAGCTCCTGTTATCCAGCAGGTCTTAGCCCCA CTTCCTGGAGGGATTTCACCACAGACAGGCGTCATCATCCAGCCACAGCAAATCTTATTTACAGGAAATA AGACTCAGGTCATTCCTACAACAGTGGCAGCACCCGCCCCGGCACAAGCGCCGATGCCTGCAGCCGGACA GCAGCAGCCACAGGCCCAGCCTGCCCAACAGCAAGCTCCGTTGGTGCTGCAAGTTGATGGAACAGGGGAT ACATCTTCTGAGGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAAGAAGATAAAGAGAAAG ATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCGCTCAACAGCGAGGATGACGTCAGTGTCGAGGAAGG GCAGGAACTCTTTGACACTGAGAATGTTGTCGTCTGCCAGTATGATAAGATACACAGAAGTAAAAATAAA TGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAACGGAAGAGATTATATATTTTCCAAAGCCATTG GAGATGCAGAGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223402 representing NM_175335
Red=Cloning site Green=Tags(s) MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQV QQQHQPQQQQHHHHHHQHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLLQHMNASSITSAAA TAATLALPAGVTPVQQLLTNSGQLLQVVRAANGAQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAP LPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPAPAQAPMPAAGQQQPQAQPAQQQAPLVLQVDGTGD TSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSVEEGQELFDTENVVVCQYDKIHRSKNK WKFHLKDGIMNLNGRDYIFSKAIGDAEW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175335 |
ORF Size | 1017 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175335.3, NP_780544.1 |
RefSeq Size | 5691 bp |
RefSeq ORF | 1020 bp |
Locus ID | 83602 |
UniProt ID | Q99PM3 |
Gene Summary | TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211964 | Gtf2a1 (untagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR223402 | Gtf2a1 (Myc-DDK-tagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2 |
CNY 3,800.00 |
|
MR223402L3 | Lenti ORF clone of Gtf2a1 (Myc-DDK-tagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2 |
CNY 5,700.00 |
|
MR223402L4 | Lenti ORF clone of Gtf2a1 (mGFP-tagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2 |
CNY 5,700.00 |