Gtf2a1 (NM_175335) Mouse Untagged Clone

CAT#: MC211964

Gtf2a1 (untagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2, (10ug)



  "NM_175335" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6330549H03Rik; AA536742; AA959775; AW060250; Tfiia1; TfIIAa/b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211964 representing NM_175335
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACTAAAAACTTTGTGGGAGAATAAACTAATGCAGTCTAGGGCAGTAGATGGATTTCATTCAGAAG
AACAGCAGCTTTTGTTGCAAGTTCAGCAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCACCACCA
CCAGCACCAGCAAGCGCAACCTCAGCAAACGGTACCTCAGCAAGCACAGACCCAGCAGGTCCTCATTCCT
GCATCCCAGCAAGCTACAGCACCACAAGTTATTGTTCCTGATTCTAAGTTGCTACAACATATGAATGCAT
CAAGTATTACGAGTGCTGCTGCTACAGCTGCTACCTTGGCACTGCCTGCAGGAGTGACTCCGGTTCAACA
GCTACTAACAAATTCAGGCCAGCTGCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAGTATATCTTGCAG
CCTCAGCAGTCAGTTGTTCTACAACAACAAGTTATACCACAAATGCAGCCTGGTGGAGTCCAAGCTCCTG
TTATCCAGCAGGTCTTAGCCCCACTTCCTGGAGGGATTTCACCACAGACAGGCGTCATCATCCAGCCACA
GCAAATCTTATTTACAGGAAATAAGACTCAGGTCATTCCTACAACAGTGGCAGCACCCGCCCCGGCACAA
GCGCCGATGCCTGCAGCCGGACAGCAGCAGCCACAGGCCCAGCCTGCCCAACAGCAAGCTCCGTTGGTGC
TGCAAGTTGATGGAACAGGGGATACATCTTCTGAGGAAGATGAAGATGAAGAAGAAGACTATGATGATGA
TGAGGAAGAAGATAAAGAGAAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCGCTCAACAGCGAG
GATGACGTCAGTGACGAGGAAGGGCAGGAACTCTTTGACACTGAGAATGTTGTCGTCTGCCAGTATGATA
AGATACACAGAAGTAAAAATAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAACGGAAGAGA
TTATATATTTTCCAAAGCCATTGGAGATGCAGAGTGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175335
Insert Size 1020 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175335.3, NP_780544.1
RefSeq Size 5691 bp
RefSeq ORF 1020 bp
Locus ID 83602
UniProt ID Q99PM3
Gene Summary TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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