United States
Germany
Japan
United Kingdom
China
Ube3c (NM_133907) Mouse Tagged ORF Clone
CAT＃: MG223355
- TrueORF®
Ube3c (tGFP-tagged) - Mouse ubiquitin protein ligase E3C (Ube3c), (10ug)
ORF Plasmid: DDK
"NM_133907" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI853514; mKIAA0010 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223355 representing NM_133907
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTTCGAAGGCGACTTCAAGACAAGGCCCAAAGTGTCTCTCGGTGGCGCGAGCAGGAAGGAAG AAAAGGCTTCTCTTTTACATCGCACTCAGGAAGAAAGACGCAAGAGAGAGGAAGAAAGGCGAAGATTGAA AAATGCAGTAATAATCCAATCTTTTATCCGAGGCTATAGAGACAGAAAACAACAATACTTCATCCAAAGA AGCGCGTTTGACCAATGCACTGACTCGGCTCAGCCTGGTGGTACCTTCTGCCTTGCTGATGGTCCCAACC TTACCCTCTTGGTAAGGCAGCTTCTGTTCTTTTACAAGCAGAGTGAAGACTCCAAACGTTTGATATGGCT TTATCAGAACTTAATTAAACACAGCTCTCTGTTTGTCAAGCAATTGGATGGATCAGAGAGACTGACATGC TTATTTCAAATAAAGAGGTTGATGAGCCTCTGTTGCAGGTTGCTGCAAAACTGTAGTGATGACAGTCTGA ATGTTGCACTTCCAATGCGAATGCTTGAGGTGTTTACATCTGAGAATACATACTTGCCTGTTTTACAAGA TTCTAGCTATGTGGTGTCAGTGATTGAACAAATTTTGCACTACATGGTTCACAGTGGATATTATAGATCT CTCTACTTGTTGATTAATAGCAAGCTTCCATCAAGTATTGAATATTCTGATTTATCTCGAGTTCCTATAG CAAAAATTTTGCTGGAGAATGTCCTAAAACCATTGCACTTTACGTATAGCTCCTGTCCTGAAGCCTCAAG GCATCAAGTTTTCTCAGCCTTCACAGAGGAGTTTCTGGGAGCACCTTTTACCGATCAAATTTTTCATTTC GTCATTCCGGCCTTTGCAGATGCACAAACTGTGTTCCCTTATGAGCCCTTTCTCAATGCATTGTTGTTAC TTGAGAGTCAAAGTTCAAAACGGTGTAGTGGAGTGCCATGGCTCTTCTATTTTGTTTTAACTGTTGGCGA GAATTATTTAGGAGCACTCTCTGAGGATGGTCTACTGGTTTATCTGCGAGTGCTTCAGACCTTCCTCTCT CAGCTACCTGCTTCACCCACTGGTACAGGCTGTCCTGATTCCACCAGTGACTCTGAGGACGATAACGAGG AGACTGACCAACCCAACAGCCCTGAGGATGGCAGGGTGTCAGCCCCATATATTACTGAAGAGTGTCTGAG AAAACTGGATACGAAGCAGCAAACCAACACCCTGCTGAACCTGGTGTGGAGGGACTCGGCCAGTGAGGAG GTCTTCACAAGGATGGCTTCTATCTGCCACACATTGATGGTGCAGCACCGAATGATGGTGCCCAAAGTCA GGCTTCTCTACAGTTTAGCCTTCAATGCCAGGTTTTTGAGGCATCTTTGGTTTCTCATCTCTTCAATGAC AACACAGATGATCACAGGATCTATGGTGCCATTGCTTCAGTTGATATCGCGGGGTTCGCCCATGTCTTTT GAAGATTCTAGTCGAATCATCCCACTCTTTTACCTCTTCAGCTCCTTGTTCAGTCATTCGCTGATCTCCA TACATGACAATGAATTTTTTGGTGACCCCATAGAAGTTGTAGGTCAGAGACAATCATCCATGATGCCTTT CACTCTGGAGGAGTTAATCCTGCTGTCACGATGCCTTCGAGATGCCTGCTTGGGGATCATCAAGCTAGCA TACCCGGAAACAAAGCCAGAAGTTCGGGAAGAATATGTCACCGCATTTCAGAGTATTGGGGTCACTACTA ATTCTGAGATGCAGCAGTGCATACAAATGGAACAGAAGCGATGGGTTCAGCTGTTCAAGGTTATCACCAA CCTAGTGAAAATGCTGAAGTCTAGAGACACGAGGAGAAATTTCTGTCCTCCAAACCACTGGCTGTCAGAA CAAGAGGATATCAAAGCAGATAAAGTTACCCAGCTCTATGTACCTGCATCTAGACATGTGTGGCGATTCC GGAGGATGGGCAGGATTGGCCCACTGCAGTCCACCCTGGAGGTGGGTCTGGAGTCCTTACCCCTGTCTGT GTCTGAGGAACGACAGCTTGCCATCCTGACAGAACTGCCTTTTGTGGTTCCATTTGAGGAGCGAGTGAAG ATCTTTCAAAGGTTGATTTATGCTGATAAGCAAGAAGTTCAAGGCGATGGTCCATTTCTGGATGGAATCA ATGTTACTATAAGGAGAAATTATATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGA TTTGAAAAAACGGATCCGGGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATCGATGGTGGT GGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAATCTGGATTTAACCCCAACCAGGGCTTCTTTAAGA CTACTAATGAAGGGCTTCTATACCCCAACCCAGCTGCTCAGATGCTTGTGGGAGATTCTTTCGCAAGGCA TTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTGTATGAAAACATGTTGGTGGAGCTACCCTTTGCT GGCTTTTTCCTGTCCAAGCTACTGGGAACCAGTGCAGATGTGGACATTCACCATCTTGCCTCATTGGACC CCGAAGTCTACAGGAACCTGCTTTTCCTCAAGAGCTATGAGGAGGATGTGGAGGAACTGGGACTGAACTT TACTGTGGTGAACAATGACCTGGGAGAAGCCCAGGTGGTTGAACTAAAATTTGGTGGAAAAGACATCCCG GTAACTGGGGCCAACCGAATTGCCTACATCCACCTGGTGGCTGACTACCGGCTGAACAAGCAGATCCGAC CGCATTGCCTAGCTTTCCGCCAGGGCCTGGCCAATGTTGTGAGCCTAGAGTGGCTCCGAATGTTTGACCA ACAGGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCTGTAAGTCTGGAGGACCTAAAATCCTTTACA AACTATTCAGGGGGCTATTCTGCCGATCATCCTGTCATTAAAATCTTTTGGAGAGTTGTAGAAGGTTTCA CTGACGAAGAAAAGCGCAAGTTGCTCAAGTTTGTAACAAGTTGCTCTCGCCCCCCTCTCCTGGGTTTCAA GGAGCTATACCCTGCGTTTTGCATTCACAATGGAGGCTCTGACCTTGAGCGGCTACCTACAGCCAGCACC TGCATGAACCTGCTGAAGCTCCCAGAGTTTTATGATGAAGCCCTGCTGCGAAGCAAGCTGCTGTATGCCA TTGAGTGTGCTGCTGGCTTTGAGCTGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223355 representing NM_133907
Red=Cloning site Green=Tags(s) MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAVIIQSFIRGYRDRKQQYFIQR SAFDQCTDSAQPGGTFCLADGPNLTLLVRQLLFFYKQSEDSKRLIWLYQNLIKHSSLFVKQLDGSERLTC LFQIKRLMSLCCRLLQNCSDDSLNVALPMRMLEVFTSENTYLPVLQDSSYVVSVIEQILHYMVHSGYYRS LYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYSSCPEASRHQVFSAFTEEFLGAPFTDQIFHF VIPAFADAQTVFPYEPFLNALLLLESQSSKRCSGVPWLFYFVLTVGENYLGALSEDGLLVYLRVLQTFLS QLPASPTGTGCPDSTSDSEDDNEETDQPNSPEDGRVSAPYITEECLRKLDTKQQTNTLLNLVWRDSASEE VFTRMASICHTLMVQHRMMVPKVRLLYSLAFNARFLRHLWFLISSMTTQMITGSMVPLLQLISRGSPMSF EDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELILLSRCLRDACLGIIKLA YPETKPEVREEYVTAFQSIGVTTNSEMQQCIQMEQKRWVQLFKVITNLVKMLKSRDTRRNFCPPNHWLSE QEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSTLEVGLESLPLSVSEERQLAILTELPFVVPFEERVK IFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGG GIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFA GFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEEDVEELGLNFTVVNNDLGEAQVVELKFGGKDIP VTGANRIAYIHLVADYRLNKQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPVSLEDLKSFT NYSGGYSADHPVIKIFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTAST CMNLLKLPEFYDEALLRSKLLYAIECAAGFELS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133907 |
| ORF Size | 3249 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133907.3, NP_598668.1 |
| RefSeq Size | 5033 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 100763 |
| UniProt ID | Q80U95 |
| Gene Summary | E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223487 | Ube3c (untagged) - Mouse ubiquitin protein ligase E3C (Ube3c), (10ug) |
CNY 13,870.00 |
|
| MR223355 | Ube3c (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3C (Ube3c) |
CNY 7,552.00 |
|
| MR223355L3 | Lenti ORF clone of Ube3c (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3C (Ube3c) |
CNY 11,400.00 |
|
| MR223355L4 | Lenti ORF clone of Ube3c (mGFP-tagged) - Mouse ubiquitin protein ligase E3C (Ube3c) |
CNY 11,400.00 |
