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Kdm5d (NM_011419) Mouse Tagged ORF Clone
CAT＃: MG223231
- TrueORF®
Kdm5d (tGFP-tagged) - Mouse lysine (K)-specific demethylase 5D (Kdm5d), (10ug)
ORF Plasmid: DDK
"NM_011419" in other vectors (4)
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CNY 14,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HY; Jarid1d; Smcy |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223231 representing NM_011419
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCAGGATCTGACGACTTTCTACCGCCGCCTGAGTGCCCTGTGTTTGAGCCCAGTTGGGCGGAAT TCCGCGATCCTCTTGGCTATATAGCTAAAATCAGACCCATTGCGGAGAAATCAGGCATTTGCAAGATACG CCCACCTGCGGATTGGCAACCTCCTTTTGCTGTAGAAGTTGATAACTTCAGATTTACTCCTCGAATTCAG AGGCTAAATGAACTGGAGGCCCAGACCAGAGTAAAACTGAACTATTTGGATCAGATTGCAAAGTTCTGGG AAATTCAAGGTTCTTCCTTAAAGATACCTAATGTGGAGAGGAAGATCTTGGACCTTTACAGCCTTAATAA GATTGTGATGGAGGAAGGTGGATATGAAGCAATCTGCAAGGACCGTCGGTGGGCCCGTGTTGCCCAACGC CTTAATTACCCATCAGGGAAAAACATTGGTTCCCTGCTTCGGTCCCACTACGAACGGATCATTTACCCTT ATGAAATATTTCAGTCTGGAGCTAATCTTGTGCAATGTAACACAGATCCATTTGACAGTGAAGAAAGAGA CAAGGAATACAAACCTCATAGCATTCCTCTTAGACAGTCTGTTCAGCCATCAAAATTCAGTTGCTACAGT AGACGAGGAAAAAGGCTACAGCCTGAGCCAGAACCCACAGAAGAAGACATTGAGAAAAACCCAGAGCTAA AGAAACTACAGATATATGGGGCAGGCCCCAAGATGATAGGCTTGGGCCTCAAGGCTAAGGAGAAAACTCT GAGAAAGAAAGATAGCAAACAACCAGATAAAGAAGAAGTGACATGCCCTGCAACTATTGTAGTGAAGGGG GAAGCCAGTGAGTTTGGAAAGGTGACATCAGCATTTTCAGACAAGAACTTAAATCACAGTTTTGAACCTT GTATGAAGATGACTATGCAGCTACGGAACAATCACAGCAGTACCCAGTTTATGAATTCATATGTTTGCCG AATATGCTCTCGTGGGGATGAAGTCGATAAGTTTCTGCTCTGTGATGGCTGCAGTGATAATTACCACATC TTTTGCCTATTGCCACCGCTTTCTGAAGTCCCCAAGGGAGTATGGAGATGCCCGAAGTGTATCTTAGCAG AGTGTAAGAGTCCCCCTGAAGCTTTTGGCTTTGAGCAGGCTACACAGGAGTATACTTTGCAGAGTTTTGG TGAGATGGCTGACTCCTTCAAGGCTGACTACTTCAACATGCCTGTGCATATGGTACCTACAGAAGTTGTG GAAAAGGAATTCTGGAGGCTGGTGAGCAGCATTGAGGAGGATGTGACAGTTGAATATGGAGCAGACATTC ACTCCAAAGAATTTGGCAGTGGGTTTCCTGTCAACAATAGCAAATGGGACTTATCTCCTGAAGAAAAGGA ATATGCTGCTTGTGGTTGGAATCTCAATGTGATGCCAGTGCTGGATCAGTCTGTTCTCTGCCACATCAAT GCAGACATCTCAGGCATGAAAGTGCCCTGGTTATATGTGGGCATGGTGTTTTCAGCATTTTGTTGGCATA TTGAGGATCACTGGAGTTATTCCATTAACTACCTGCACTGGGGTGAACCAAAGACCTGGTATGGAGTACC TTCGCTAGCAGCAGAACACTTAGAGGATGTAATGAAGAGACTTACACCAGAGTTGTTTGACAGCCAACCT GACCTCCTGCACCAACTTGTCACTCTGATGAATCCTAACACTCTCATGTCACATGGAGTGCCAGTTGTTC GTACAAACCAGTGTGCAGGGGAATTTGTCATTACTTTTCCCCGTGCTTACCACAGTGGCTTTAACCAAGG CTATAACTTTGCTGAGGCAGTGAACTTTTGCACTGCCGATTGGCTACCTGTTGGACGCCAGTGTATTGAA CACTACCGCCGACTCCGGCGCTATTGTGTCTTCTCCCATGAAGAACTTATCTGCAAGATGGCTGCATTTC CAGAGAAGTTGGATCTAAATCTAGCAGTGGCAGTTCACAAGGAGATGTTCATTATGGTACAGGAGGAGCG ACGCCTACGAAAGACCTTGTTGGAGAAGGGCATCACGGAAGCTGAGCGAGAGGCTTTTGAGCTACTCCCA GATGATGAGCGTCAGTGCATCAAGTGTAAGACCACGTGTTTCCTATCAGCCTTGGCCTGTTATGACTGTC CAGATAGTCTTGTCTGCCTTTCCCATATCAATGATCTGTGCAAGTGCTCAAGGAATCGACAGTACTTACG GTACAGGTACACATTGGATGAACTGCCTGCTATGCTACAAAAGCTGAAGATTCGAGCTGAGTCATTTGAC AACTGGGCCAACAAAGTACAAGCAGCCCTAGAGGTGGAGGATGGTCGGAAACGGAGTTTTGAAGAGCTAA GAGCATTGGAATCTGAGGCTCGTGATAGACGATTTCCTAATAGTGAGCTGCTTCAGCGATTGAAGAAATG CCTAACTGAAGCAGAGGCTTGTATTTCCCAAGTTCTTGGACTGATCAGTAACTCAGAGGACAGGTTACAA ACACCGCAAATCACCCTTACTGAACTACAGCTTCTTCTCAAACAGATGGGCACTCTGCCCTGTACCATGC ATCAGATTGATGAAGTTAAGGATGTTCTTCAACAGGTGGAATCATACCAAATTGAGACCCGTGAGGCTCT AACTTCACTGCCTTACAGTTTAGAAATACTTCAGTCCCTGATGGAAAAGGGTCAGCAGCTTCGTGTGGAA GTGCCTGAAGCTCATCAACTTGAAGAGCTGTTGGAGCAGGCACAATGGCTAGATCAAGTGAAGCAGGCCC TGGCTCCCTCAGGCCAGAGGCACTCTTTGGTTATCATGAAGAAGCTTTTAGTTATGGGAACCAAGGTAGC CTCCAGCCCTTCTGTGAACAAAGCTCGAGCAGAGTTACAAGAACTGCTGACTATTGCAGAATGTTGGGAG GAAAAGGCCCATTTTTGCTTAAAAGCTAGCCAAAAGCATTCTCCAGCCACTTTGGAAGTTATAATACGTG AGGCAGAAAACATCCCTGTCTACCTACCTAACATCCAGTCTCTGAAGGAAGCTCTGACTAAGGCACAGGC TTGGATTGCTGATGTGAATGAGATTCAAAATGGTGATCACTACCCCTGTTTGGATGATTTGGAGGGCCTT GTAGCTGTTGGTCGGGATTTACCTGTGGAGCTAGAGGAATTGAGACAGCTGGAGAATCAGGTACTAACAG CACATTCCTGGAAAGAGAAAGCTTCTAAGACTTTTCTTAAGAAGAATTCTTGCTACACATTGTTGGAGGT TCTCTGCCCATGTGCAGATGCTGGTTCAGTCAGCACCAAGCGCAGTCGGTGGATTGAAAAGGAGATGGGA TTATACAAATATGACACAGAATTGCTGGGGCTGTCTGCACAGGACCTCAGGGACCCAGGGTCTGTGATCA TGGCCTTTAAGGAAGGGGAAGAGAAAGAAAAGGAAGGTATCTTGCACTTACGTCACATCAACTCAGCCAA GCCCAGTCCAATGTCCTCATCGATGAATGCCTCTGCAACCTCCATCTGTATATGTGGGCAGGTCTGTGCA GGAGTGGAATCTCTGCAGTGTGATCTTTGTCATGACTGGTTCCATGGACAATGTGTGACTGTACCCCACC TACTCAGCTCAGTAAGGGCCAGTCATACCTCATCTCAACTGCTAGCTTGGTGGGAATGGGACACCAAGTT CCTGTGCCCACTGTGCATGCGATCAAGGCGTCCACGCCTGGAGACCATCTTGTCATTGCTGGTAGGACTA CAGAGACTGTCTGTAAGGCTACCTGAGGGTGAGGCCCTACAGTGCCTCACAGAGAGAGCCATTGGTTGGC AAGGCCGTGCCAGGCAGGCTCTAGCTTCTGAGGATGTGACTGCTTTGTTGAAACAGCTGGAAAAATCCCG CCAACAACTACAGGATGAATTGAGACATAAGAAGCCCCCTACCTTGCCTTCAGGCTTTGCCTTTGACTGT CTCACAGAGAATAGTGGCAAGGATATCCTTAAGGAAGAGGAGGAGTTGGTACTAAATGAAGAAAGGATTA AAAGTTCTGAGAAGATTGTTCCAAAGGAAAGTTCATGTAAAGGAGATAAGGAACTGCTGCCTTCACTTTT ATCCCAATTAACTGGCCCAGTACTGGAACTGCCTGAAGCCACCCGGGCACCCCTAGAGGAGCTTATGATG GAAGGAGATCTACTTGAAGTTACCCTAGATGAGAACTACAGCATTTGGCAGCTGCTGCAGGCTGGGCAGA ATCCCAATCTAGAGCGCATTCATACACTTCTGGAGCTTGAGAAGCCTGAAAACCCAGGAAATTGGTCAGA GGAACAGACACCAGAGAGGCGACGGCAGAGGAGGCAAAAGGTGGTTCTGAGTAGAAAGGGTGAGGATTTT ACTCAAAAAGAGCTAGAATCAAAAAGAGTCAAGAGTTCAAGGATTAAGCCTAAGGAAGAAAAGTTTCAAA AGCCAATACTTGGGGATAATGTGCTGTACACACACCATACAGAACATACAAACATCTTAAAAGAGCACAT AAACAGTGTACAAGGAAAGGATCCAAGCCCTTCATCTTCTTTTCCTTCCTTAACACCTTTACTACACCTA TCTTATTTTCATCAGCAAAAATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223231 representing NM_011419
Red=Cloning site Green=Tags(s) MKPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEVDNFRFTPRIQ RLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERKILDLYSLNKIVMEEGGYEAICKDRRWARVAQR LNYPSGKNIGSLLRSHYERIIYPYEIFQSGANLVQCNTDPFDSEERDKEYKPHSIPLRQSVQPSKFSCYS RRGKRLQPEPEPTEEDIEKNPELKKLQIYGAGPKMIGLGLKAKEKTLRKKDSKQPDKEEVTCPATIVVKG EASEFGKVTSAFSDKNLNHSFEPCMKMTMQLRNNHSSTQFMNSYVCRICSRGDEVDKFLLCDGCSDNYHI FCLLPPLSEVPKGVWRCPKCILAECKSPPEAFGFEQATQEYTLQSFGEMADSFKADYFNMPVHMVPTEVV EKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVNNSKWDLSPEEKEYAACGWNLNVMPVLDQSVLCHIN ADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPSLAAEHLEDVMKRLTPELFDSQP DLLHQLVTLMNPNTLMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPVGRQCIE HYRRLRRYCVFSHEELICKMAAFPEKLDLNLAVAVHKEMFIMVQEERRLRKTLLEKGITEAEREAFELLP DDERQCIKCKTTCFLSALACYDCPDSLVCLSHINDLCKCSRNRQYLRYRYTLDELPAMLQKLKIRAESFD NWANKVQAALEVEDGRKRSFEELRALESEARDRRFPNSELLQRLKKCLTEAEACISQVLGLISNSEDRLQ TPQITLTELQLLLKQMGTLPCTMHQIDEVKDVLQQVESYQIETREALTSLPYSLEILQSLMEKGQQLRVE VPEAHQLEELLEQAQWLDQVKQALAPSGQRHSLVIMKKLLVMGTKVASSPSVNKARAELQELLTIAECWE EKAHFCLKASQKHSPATLEVIIREAENIPVYLPNIQSLKEALTKAQAWIADVNEIQNGDHYPCLDDLEGL VAVGRDLPVELEELRQLENQVLTAHSWKEKASKTFLKKNSCYTLLEVLCPCADAGSVSTKRSRWIEKEMG LYKYDTELLGLSAQDLRDPGSVIMAFKEGEEKEKEGILHLRHINSAKPSPMSSSMNASATSICICGQVCA GVESLQCDLCHDWFHGQCVTVPHLLSSVRASHTSSQLLAWWEWDTKFLCPLCMRSRRPRLETILSLLVGL QRLSVRLPEGEALQCLTERAIGWQGRARQALASEDVTALLKQLEKSRQQLQDELRHKKPPTLPSGFAFDC LTENSGKDILKEEEELVLNEERIKSSEKIVPKESSCKGDKELLPSLLSQLTGPVLELPEATRAPLEELMM EGDLLEVTLDENYSIWQLLQAGQNPNLERIHTLLELEKPENPGNWSEEQTPERRRQRRQKVVLSRKGEDF TQKELESKRVKSSRIKPKEEKFQKPILGDNVLYTHHTEHTNILKEHINSVQGKDPSPSSSFPSLTPLLHL SYFHQQKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011419 |
| ORF Size | 4644 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011419.3, NP_035549.1 |
| RefSeq Size | 5471 bp |
| RefSeq ORF | 4647 bp |
| Locus ID | 20592 |
| UniProt ID | Q62240 |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis. Involved in transcriptional repression of diverse metastasis-associated genes; in this function seems to cooperate with ZMYND8. Suppresses prostate cancer cell invasion. Regulates androgen receptor (AR) transcriptional activity by demethylating H3K4me3 active transcription marks (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224671 | Kdm5d (untagged) - Mouse lysine (K)-specific demethylase 5D (Kdm5d), (10ug) |
CNY 19,950.00 |
|
| MR223231 | Kdm5d (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 5D (Kdm5d) |
CNY 16,944.00 |
|
| MR223231L3 | Lenti ORF clone of Kdm5d (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 5D (Kdm5d) |
CNY 19,344.00 |
|
| MR223231L4 | Lenti ORF clone of Kdm5d (mGFP-tagged) - Mouse lysine (K)-specific demethylase 5D (Kdm5d) |
CNY 19,344.00 |
