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E2f7 (NM_178609) Mouse Tagged ORF Clone
CAT＃: MG222951
- TrueORF®
E2f7 (tGFP-tagged) - Mouse E2F transcription factor 7 (E2f7), (10ug)
ORF Plasmid: DDK
"NM_178609" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A630014C11Rik; D10Ertd739e; E2F-7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222951 representing NM_178609
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGAATTGTTTAACACTAAAAGACTTGATCAGCCCCAGGCAGACCAGACTAGATTTTGCCATTG AAGATGCAGAGAACGCACAAAAGGAAAATATATTTGTCGACCGATCAAGGATGACCCCAAAGACACCGAT GAAGAACGAGCCGATCGACCTGTCAAAGCAAAGAATCTTCACCCCAGACAGAAACCCCATTACTCCAGTA AAGCCGGTCGACAGGCAGCCGCAGGTGGAGCCCTGGACACCCACAGCCAACCTGAAGATGCTCATCAGCG CCGCCAGCCCAGACATAAGAGACCGGGAGAAGAAAAAGGAGCTGTTCAGACCCATTGAGAATAAGGAGGA TGCGTTCGTGAACTCCCTGCAGCTTGACGTGGCCGGTGACGGCGCTGTGGATGAGTATGAGAAGCAAAGG CCAAGCAGGAAACAGAAGAGCTTGGGGCTGCTGTGCCAGAAGTTTCTAGCTCGCTATCCGAGCTACCCCT TGTCAACGGAGAAAACCACCATCTCCCTAGATGAGGTCGCCGTCAGTCTCGGCGTGGAAAGGAGACGCAT CTATGACATCGTAAACGTGCTGGAGTCGCTGCATCTGGTCAGCCGGGTGGCTAAGAATCAGTATGGTTGG CACGGTCGGCACAGCCTCCCCAAAACCCTGAGGACCCTACAGAGACTGGGAGAGGAACAGAAATACGAGG AGCAGATGGCCTGCCTCCAGCAGAAGGAGCTGGACCTGATGGGGTATAGGTTCGGAGAACGCAGGAAAGA CGGGTCTCCGGACCCCCGAGATCCACTCCTACTTGATTTTTCCGAAGCCGACTACCCCTCTTCATCTGCA AACAGTCGAAAAGATAAATCTTTAAGAATTATGAGCCAGAAGTTTGTCATGCTGTTCCTCGTCTCCAAAA CCAAGATTGTCACCCTGGATGTAGCTGCCAAAATCCTCATAGAAGAAAGCCAAGATACCCCGGACCACAG CAAATTCAAAACAAAGGTACGGAGGCTCTATGACATAGCCAATGTTCTGACCAGCCTGGCTCTGATAAAG AAAGTTCACGTAACAGAAGAGCGAGGCCGGAAACCAGCCTTCAAGTGGATCGGGCCTGTGGACTTCAGCT CCATTGATGAAGAACTGTTGGACGTCTCTGCATCCATCTTACCAGAATTGAAGAAGGAAGCATACGGCCA GATCCGAGTGTGTGCAAAAGAGAGGCTGGTGCGGTACGGTTCCTTTAACACGGTTCACACGTCTGAGAAG ATACAGAGAAAAGTGAGCTCAGAACCCAGCAGCCCACAGGGAGAAAGACAAGGGTCAGCCTATTCCCTGG AAATTGGAAGTCTGGCGGCCATCTACAGACAGAAAGTAGAAGATAATTCGCAGGAGGAAGCCTTTGTCAG TAACACAGCAGTGCCTCCAGCAAGCATCTTGGACCCCGCCCTCTCCATGGACTCTGAATACTGTGTCAAG CCTTTAGCCCAGCCAGTATTTTCTGTTGCTCAAACAGATTTGCCGGCCTTCTCTGCGCAGAATGGTCCAA GCGGACAAGTAGGTGTCCCCGTTCCTTCTGCAGCCTCCGACACAGAGAACTTAAAGCCAGCTCTGCTCGC CGGCCAGCCCCTGGTCTACGTGCCCTCCACCCAACTCTTCATGCTGTACGGGAGTGTGCAGGAGGGACTG TCCCCAGAGTCAAGGTCAGAGGAAGATGGTGGTGGCTCCGATGTCCCAGCTGACCTGTCAGTGACACCCT CTGCTCAGAAGCGCCTGTGTGAGGAGAGAGATCCTCAGGAGGAGGAGGACGAGCCCGCCATGAAAAGACA AAGTCAGGAATTTGAAGACAGCCCCTTATCCCTAGTTATGCCCAAGAAACCCTCAAGTTCCACAGACCTT GCCTGTCCTGTGACCATGGGAAACGGGAGCTCTCCACCCCTGGAAGACGCCTGTGTTAAGGGTCAGCTCC CTGCTGCAGAAGAAGTTACAGGAAAGGCTGCCCCAAACTGCTATGTTGCTTCTGAGTGTGGAAACCCGGC AAGAAATCCAGACACTGAAAAGCCTTCCAACGAAAACGAGATCACCAAAGACCCCTCGCTGATGCAGTAC CTGTATGTGCAGTCTCCCGCAGGGTTAAATGGTTTTAACATGGTTTTACCAGGCACCCAGACCCCCCACA CCGTGGCACCCTCCCCGGCTCAGCTGCCATCCTTCGGCGTACCCTGCATGTTCCTGCAATCCCCAGGCCT GGGCCCTTTTCCAGTTCTCTATTCTCCCGCAATACCCGGGCCCATTTCTTCAGCCCCCGGCACTCACCCA AACCCCGGACCCATGAACTTCGGCTTGTCCACCCTGGCATCTGCTTCTCACCTTCTCATCAGCCCGGCAG CCATGGTCAATCCAAAGCCATCCACCCTCCCCTGTACAGACCCTCAGCTTCGCTGTCAGCCCTCACTCAA CCTGAACCCCGTGATGCCAGGCTCACACGGCGTCATCCACCCCGAGTCCCCCTGTTACGTGAGACATCCG GTATCGATGGTAAAGGCAGAGCAGTCCCCTGCACCGGCGACTCCCAAGAGCATCCAACGGAGACATCGGG AGACGTTCTTCAAGACTCCCGGCAGCCTCGGCGATCCGGTCTTTAGAAGAAAAGAAAGGAACCAATCAAG AAACACTAGCTCAGCCCAGAGGAGACTAGAAATCTCTAGCAGCGGGCCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222951 representing NM_178609
Red=Cloning site Green=Tags(s) MEVNCLTLKDLISPRQTRLDFAIEDAENAQKENIFVDRSRMTPKTPMKNEPIDLSKQRIFTPDRNPITPV KPVDRQPQVEPWTPTANLKMLISAASPDIRDREKKKELFRPIENKEDAFVNSLQLDVAGDGAVDEYEKQR PSRKQKSLGLLCQKFLARYPSYPLSTEKTTISLDEVAVSLGVERRRIYDIVNVLESLHLVSRVAKNQYGW HGRHSLPKTLRTLQRLGEEQKYEEQMACLQQKELDLMGYRFGERRKDGSPDPRDPLLLDFSEADYPSSSA NSRKDKSLRIMSQKFVMLFLVSKTKIVTLDVAAKILIEESQDTPDHSKFKTKVRRLYDIANVLTSLALIK KVHVTEERGRKPAFKWIGPVDFSSIDEELLDVSASILPELKKEAYGQIRVCAKERLVRYGSFNTVHTSEK IQRKVSSEPSSPQGERQGSAYSLEIGSLAAIYRQKVEDNSQEEAFVSNTAVPPASILDPALSMDSEYCVK PLAQPVFSVAQTDLPAFSAQNGPSGQVGVPVPSAASDTENLKPALLAGQPLVYVPSTQLFMLYGSVQEGL SPESRSEEDGGGSDVPADLSVTPSAQKRLCEERDPQEEEDEPAMKRQSQEFEDSPLSLVMPKKPSSSTDL ACPVTMGNGSSPPLEDACVKGQLPAAEEVTGKAAPNCYVASECGNPARNPDTEKPSNENEITKDPSLMQY LYVQSPAGLNGFNMVLPGTQTPHTVAPSPAQLPSFGVPCMFLQSPGLGPFPVLYSPAIPGPISSAPGTHP NPGPMNFGLSTLASASHLLISPAAMVNPKPSTLPCTDPQLRCQPSLNLNPVMPGSHGVIHPESPCYVRHP VSMVKAEQSPAPATPKSIQRRHRETFFKTPGSLGDPVFRRKERNQSRNTSSAQRRLEISSSGPD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178609 |
| ORF Size | 2712 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178609.4, NP_848724.2 |
| RefSeq Size | 5476 bp |
| RefSeq ORF | 2715 bp |
| Locus ID | 52679 |
| UniProt ID | Q6S7F2 |
| Gene Summary | Atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in DNA damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in DNA replication. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. Acts as a negative regulator of keratinocyte differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222527 | E2f7 (untagged) - Mouse E2F transcription factor 7 (E2f7), (10ug) |
CNY 11,690.00 |
|
| MR222951 | E2f7 (Myc-DDK-tagged) - Mouse E2F transcription factor 7 (E2f7) |
CNY 6,624.00 |
|
| MR222951L3 | Lenti ORF clone of E2f7 (Myc-DDK-tagged) - Mouse E2F transcription factor 7 (E2f7) |
CNY 9,030.00 |
|
| MR222951L4 | Lenti ORF clone of E2f7 (mGFP-tagged) - Mouse E2F transcription factor 7 (E2f7) |
CNY 9,030.00 |
