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Nagpa (NM_013796) Mouse Tagged ORF Clone
CAT＃: MG222883
- TrueORF®
Nagpa (tGFP-tagged) - Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_013796" in other vectors (4)
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CNY 5,448.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI596180; UCE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222883 representing NM_013796
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCCCAGGGGGCCCGGGCTGTTCCTCATACCCGCGCTGCTCGGCTTACTCGGGGTGGCGTGGT GCAGCCTAAGCTTCGGGGTTTCCCGCGACGATGACCTGCTGCTGCCTTACCCACTAGCGCGCAGACGTCC CTCGCGAGACTGCGCCCGGGTGCGCTCAGGTAGCCCAGAGCAGGAGAGCTGGCCTCCGCCACCCACGAAC CCCGGCGCCAGCCACCACGCGGCCGTGCGCACCTTCGTGTCGCACTTCGAGGGGCGCGCGGTGGCCGGCC ACCTGACGCGGGTCGCCGATCCCCTACGCACTTTCTCGGTGCTGGAGCCCGGAGGAGCCGGGGGCTGCGC GCAGAAGCGCCGCGCTACTGTGGAGGACACAGCCGTCCCGGCCGGTTGCCGCATCGCTCAGAACGGTGGC TTCTTCCGCATGAGCACTGGCGAGTGCTTGGGGAACGTGGTGAGCGACGGGCGGCTGGTGAGCAGCTCAG GGGGACTGCAGAACGCGCAGTTCGGTATCCGACGCGATGGAACCATAGTCACCGGGTACCTGTCTGAGGA GGAGGTTCTGGATCCCGTGAATCCGTTCGTGCAGCTGCTGAGCGGAGTCGTGTGGCTCATCCGCAATGGA AACATCTACATCAACGAGAGCCAAGCCATCGAGTGTGACGAGACACAGGAGACAGGTTCTTTTAGCAAAT TTGTGAATGTGATGTCAGCCAGGACAGCCGTGGGTCATGACCGTGAGGGGCAGCTTATCCTCTTCCATGC TGATGGACAGACGGAACAGCGTGGCCTTAACCTATGGGAGATGGCAGAGTTCCTGCGTCAACAAGATGTC GTCAATGCCATCAACCTGGATGGAGGCGGTTCTGCTACTTTTGTGCTCAATGGGACCCTGGCCAGTTACC CTTCAGATCACTGCCAGGACAACATGTGGCGCTGTCCCCGCCAAGTGTCCACTGTGGTGTGTGTGCATGA ACCGCGCTGCCAGCCACCCGACTGCAGTGGCCACGGGACCTGTGTGGATGGCCACTGTGAATGCACCAGC CACTTCTGGCGGGGCGAGGCCTGCAGCGAGCTGGACTGTGGCCCCTCCAACTGCAGCCAGCATGGGCTGT GCACAGAGACTGGCTGCCACTGTGATGCTGGGTGGACAGGATCCAACTGCAGTGAAGAGTGTCCTCTGGG CTGGTATGGGCCAGGTTGCCAGAGGCCATGCCAGTGTGAGCACCAGTGTTCCTGTGACCCGCAGACTGGC AACTGCAGCATCTCCCAAGTGAGGCAGTGTCTCCAGCCAACTGAGGCTACGCCGAGGGCAGGAGAGCTGG CCTCTTTCACCAGGACCACCTGGCTAGCCCTCACCCTGACACTAATTTTCCTGCTGCTGATCAGCACTGG GGTCAACGTGTCCTTGTTCCTGGGCTCCAGGGCCGAGAGGAACCGGCACCTCGACGGGGACTATGTGTAT CACCCACTGCAGGAGGTGAACGGGGAAGCGCTGACTGCAGAGAAGGAGCACACGGAGGAAACTAGCAACC CCTTCAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222883 representing NM_013796
Red=Cloning site Green=Tags(s) MAAPRGPGLFLIPALLGLLGVAWCSLSFGVSRDDDLLLPYPLARRRPSRDCARVRSGSPEQESWPPPPTN PGASHHAAVRTFVSHFEGRAVAGHLTRVADPLRTFSVLEPGGAGGCAQKRRATVEDTAVPAGCRIAQNGG FFRMSTGECLGNVVSDGRLVSSSGGLQNAQFGIRRDGTIVTGYLSEEEVLDPVNPFVQLLSGVVWLIRNG NIYINESQAIECDETQETGSFSKFVNVMSARTAVGHDREGQLILFHADGQTEQRGLNLWEMAEFLRQQDV VNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCSGHGTCVDGHCECTS HFWRGEACSELDCGPSNCSQHGLCTETGCHCDAGWTGSNCSEECPLGWYGPGCQRPCQCEHQCSCDPQTG NCSISQVRQCLQPTEATPRAGELASFTRTTWLALTLTLIFLLLISTGVNVSLFLGSRAERNRHLDGDYVY HPLQEVNGEALTAEKEHTEETSNPFKD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013796 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013796.3, NP_038824.2 |
| RefSeq Size | 2152 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 27426 |
| UniProt ID | Q8BJ48 |
| Gene Summary | Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217298 | Nagpa (untagged) - Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa), transcript variant 1, (10ug) |
CNY 6,650.00 |
|
| MR222883 | Nagpa (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa), transcript variant 1 |
CNY 3,848.00 |
|
| MR222883L3 | Lenti ORF clone of Nagpa (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa), transcript variant 1 |
CNY 6,460.00 |
|
| MR222883L4 | Lenti ORF clone of Nagpa (mGFP-tagged) - Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa), transcript variant 1 |
CNY 6,460.00 |
