Spag16 (NM_025728) Mouse Tagged ORF Clone
CAT#: MG222547
- TrueORF®
Spag16 (tGFP-tagged) - Mouse sperm associated antigen 16 (Spag16) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_025728" in other vectors (4)
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CNY 3,420.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222547 representing NM_025728
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACCAGATCCAAACGTGACTTCATGTACAGAAAACGTTTCTGCAGCAAAATTTGACTACAAGCTAA ACAATATTTTCAGACTTCATGAACTCCCAGTGAGCTGCATCGTCATGCATCCCTGCAGAGACTATCTCAT CTCCTGTAGTGAAGATCGTCTCTGGAAGATGGTGGGCCTCCCACAGGGCAATGTACTTCTTACAGGATCT GGCCACACTGATTGGTTGTCTGGCTGCTGCTTCCATCCGAGTGGCAGCAAGTTGGCTACCTCAAGTGGTG ACAGCACAATCAAACTATGGGACCTCAACAAAGGTGAATGCACCTTGACCCTTGAAGGACACAACCATGC CGTATGGTCCTGCACCTGGCACTCCTGTGGTGACTTCGTGGCTTCTGCCTCACTGGATATGACGAGTAAA ATCTGGGATGTCAACAGTGAACGGTGCAGATACACTTTGTATGGACACACAGACTCTGTGAACAGCATTG AGTTTTTCCCTTTCTCCAACATTCTTCTCACCGCTTCTGCAGACAAGACCTTGTCTGTGTGGGATGCACG AACAGGTAAATGTGAGCAGTCTCTGTATGGTCACATGCATTCTGTCAATGATGCCACCTTCACGCCTAGG GGTCACATAATAGCATCCTGTGATGCCCGTGGAGTTACAAAGCTGTGGGACTTCCGGAAACTCATTCCAA TCGTGTCCATTGATGTGGGTCCAAGTTCGGGAAATGAGGTGAACTTTGACCAGTCAGGTCGAGTTTTAGC TCAGGCGAGTGCCAACGGGATTATCCATTTGCTGGATCTTAAATCGGGGCAAATTCACAAACTGGTGGGC CACGAGAGTGAAGTGCACAGTGTGGTGTTTTCCCACCTGGGGGAAAATCTGTATTCTGGAGGCTCTGACG GCACCATTCGGTTGTGGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222547 representing NM_025728
Red=Cloning site Green=Tags(s) MQPDPNVTSCTENVSAAKFDYKLNNIFRLHELPVSCIVMHPCRDYLISCSEDRLWKMVGLPQGNVLLTGS GHTDWLSGCCFHPSGSKLATSSGDSTIKLWDLNKGECTLTLEGHNHAVWSCTWHSCGDFVASASLDMTSK IWDVNSERCRYTLYGHTDSVNSIEFFPFSNILLTASADKTLSVWDARTGKCEQSLYGHMHSVNDATFTPR GHIIASCDARGVTKLWDFRKLIPIVSIDVGPSSGNEVNFDQSGRVLAQASANGIIHLLDLKSGQIHKLVG HESEVHSVVFSHLGENLYSGGSDGTIRLWI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025728 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025728.4, NP_080004.1 |
RefSeq Size | 1287 bp |
RefSeq ORF | 933 bp |
Locus ID | 66722 |
UniProt ID | Q8K450 |
Gene Summary | Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210424 | Spag16 (untagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR222547 | Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 3,140.00 |
|
MR222547L3 | Lenti ORF clone of Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 5,040.00 |
|
MR222547L4 | Lenti ORF clone of Spag16 (mGFP-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 5,040.00 |