Spag16 (NM_025728) Mouse Untagged Clone
CAT#: MC210424
Spag16 (untagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2, (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC210424 representing NM_025728
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACCAGATCCAAACGTGACTTCATGTACAGAAAACGTTTCTGCAGCAAAATTTGACTACAAGCTAA ACAATATTTTCAGACTTCATGAACTCCCAGTGAGCTGCATCGTCATGCATCCCTGCAGAGACTATCTCAT CTCCTGTAGTGAAGATCGTCTCTGGAAGATGGTGGGCCTCCCACAGGGCAATGTACTTCTTACAGGATCT GGCCACACTGATTGGTTGTCTGGCTGCTGCTTCCATCCGAGTGGCAGCAAGTTGGCTACCTCAAGTGGTG ACAGCACAATCAAACTATGGGACCTCAACAAAGGTGAATGCACCTTGACCCTTGAAGGACACAACCATGC CGTATGGTCCTGCACCTGGCACTCCTGTGGTGACTTCGTGGCTTCTGCCTCACTGGATATGACGAGTAAA ATCTGGGATGTCAACAGTGAACGGTGCAGATACACTTTGTATGGACACACAGACTCTGTGAACAGCATTG AGTTTTTCCCTTTCTCCAACATTCTTCTCACCGCTTCTGCAGACAAGACCTTGTCTGTGTGGGATGCACG AACAGGTAAATGTGAGCAGTCTCTGTATGGTCACATGCATTCTGTCAATGATGCCACCTTCACGCCTAGG GGTCACATAATAGCATCCTGTGATGCCCGTGGAGTTACAAAGCTGTGGGACTTCCGGAAACTCATTCCAA TCGTGTCCATTGATGTGGGTCCAAGTTCGGGAAATGAGGTGAACTTTGACCAGTCAGGTCGAGTTTTAGC TCAGGCGAGTGCCAACGGGATTATCCATTTGCTGGATCTTAAATCGGGGCAAATTCACAAACTGGTGGGC CACGAGAGTGAAGTGCACAGTGTGGTGTTTTCCCACCTGGGGGAAAATCTGTATTCTGGAGGCTCTGACG GCACCATTCGGTTGTGGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_025728 |
Insert Size | 933 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_025728.4, NP_080004.1 |
RefSeq Size | 1303 bp |
RefSeq ORF | 933 bp |
Locus ID | 66722 |
UniProt ID | Q8K450 |
Gene Summary | Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 5'-most exon in the 5' UTR and lacks a portion of the 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2; also known as Spag16S) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222547 | Spag16 (tGFP-tagged) - Mouse sperm associated antigen 16 (Spag16) transcript variant 2, (10ug) |
CNY 3,420.00 |
|
MR222547 | Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 3,140.00 |
|
MR222547L3 | Lenti ORF clone of Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 5,040.00 |
|
MR222547L4 | Lenti ORF clone of Spag16 (mGFP-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 2 |
CNY 5,040.00 |