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Prtn3 (NM_011178) Mouse Tagged ORF Clone
CAT＃: MG222354
- TrueORF®
Prtn3 (tGFP-tagged) - Mouse proteinase 3 (Prtn3), (10ug)
ORF Plasmid: DDK
"NM_011178" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mPR3; PR3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222354 representing NM_011178
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGAAGCTACCCATCCCCCAAGGGGATCCATCCCTTCCTGCTGCTTGCCCTGGTGGTTGGTGGCG CAGTCCAGGCCTCCAAGATTGTAGGTGGGCACGAGGCTCGGCCCCACTCTCGGCCTTATGTGGCATCCCT GCAGCTGAGCAGGTTCCCTGGGAGCCACTTCTGTGGTGGCACCCTGATCCACCCGAGATTCGTGCTGACA GCCGCCCACTGCCTGCAGGACATCTCCTGGCAGCTTGTGACAGTGGTGCTGGGTGCCCACGACCTGCTGA GCTCGGAGCCTGAGCAGCAGAAGTTCACCATCAGTCAGGTCTTCCAGAACAATTACAACCCCGAGGAGAA CCTCAATGACGTGCTTCTCCTCCAGCTAAACCGGACAGCCTCCCTGGGCAAGGAGGTGGCGGTGGCTTCT CTGCCCCAGCAGGACCAGACTCTGTCCCAGGGCACCCAGTGCCTGGCCATGGGCTGGGGTCGCCTGGGCA CCCAAGCACCCACGCCCCGTGTGCTGCAGGAACTGAACGTCACGGTGGTCACCTTCCTATGCCGGGAACA CAACGTGTGCACGCTGGTGCCACGGAGGGCAGCAGGCATATGCTTCGGAGACTCGGGCGGCCCCTTGATC TGCAATGGCATTCTTCATGGAGTGGACTCCTTCGTGATCCGCGAATGCGCCTCCCTCCAGTTCCCTGATT TCTTCGCCCGGGTGTCCATGTATGTGGACTGGATTCAAAACGTGCTGCGGGGCGCAGAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222354 representing NM_011178
Red=Cloning site Green=Tags(s) MAGSYPSPKGIHPFLLLALVVGGAVQASKIVGGHEARPHSRPYVASLQLSRFPGSHFCGGTLIHPRFVLT AAHCLQDISWQLVTVVLGAHDLLSSEPEQQKFTISQVFQNNYNPEENLNDVLLLQLNRTASLGKEVAVAS LPQQDQTLSQGTQCLAMGWGRLGTQAPTPRVLQELNVTVVTFLCREHNVCTLVPRRAAGICFGDSGGPLI CNGILHGVDSFVIRECASLQFPDFFARVSMYVDWIQNVLRGAEP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011178 |
| ORF Size | 762 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011178.2, NP_035308.2 |
| RefSeq Size | 983 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 19152 |
| UniProt ID | Q61096 |
| Gene Summary | Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209240 | Prtn3 (untagged) - Mouse proteinase 3 (Prtn3), (10ug) |
CNY 2,400.00 |
|
| MR222354 | Prtn3 (Myc-DDK-tagged) - Mouse proteinase 3 (Prtn3) |
CNY 2,400.00 |
|
| MR222354L3 | Lenti ORF clone of Prtn3 (Myc-DDK-tagged) - Mouse proteinase 3 (Prtn3) |
CNY 4,750.00 |
|
| MR222354L4 | Lenti ORF clone of Prtn3 (mGFP-tagged) - Mouse proteinase 3 (Prtn3) |
CNY 4,750.00 |
