Rapgef4 (NM_019688) Mouse Tagged ORF Clone
CAT#: MG222195
- TrueORF®
Rapgef4 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), (10ug)
ORF Plasmid: DDK
"NM_019688" in other vectors (4)
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CNY 9,500.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222195 representing NM_019688
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCGCTGCGCACGCTGCACACTCTCAGTCCTCGGCCGAGTGGATCGCCTGCCTGGATAAAAGGCCGT TGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATT TCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACACTG TTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTG AGACCAGCAGTCACCAGGATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCAT TCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAG GAGGACTTCAAGGCACTATGGGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTG TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAATGTCCCTTCAGAGAAGATCCTCAG AGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCAC CTAAAGACATACAGACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTG TTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGA CCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCT TTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGA TGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGA GATTATCTACGACGAGCTCCTTCATATTAAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTA GCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTA CCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCT GCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTT CGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGG CGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAG AGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATT TTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATG ACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCA CGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATC CGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCC TGGAGGAGTTCTATGTGTCTGTATCAGATGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGA GCTGGAGAAGATTGTCAAGCAAATCTCAGAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTG CAACAGTTCAACACAGGTGACGAGAGGGCCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGT TCAAGGTCTACTGCATCGACCACACCTATACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGT CATCAGTGCAGTAGCTGACAAACTGGGCTCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGA GAAAAGGTGGTGCTGAAATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTG CCTGCCCGAGAGAGCAATTCGACTCACTGACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGT GGGAACATTTGAGCTGATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTC AACTGTGTGCATGAGCTGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAA ACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTC CCAGCTCAGCAAACGTGTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAA AATCTAAATTCCTTTTTCGCCATCGTCATGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGT GGGAGAAACTGCCGAGCAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAA CCACAGGGCATACAGGCTGACAGCAGCCAAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATT AAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGC GCATGATTGCAAACACTGCCAGAACAGTACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCA AGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACT TTATCACAGATGTCACACAGATTAGAGCCTCGAAGGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222195 representing NM_019688
Red=Cloning site Green=Tags(s) MVAAHAAHSQSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFEKFHPNLLRQICLCGYYENLEKGITL FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQ EDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRIAILSRAPHMIRDRKYH LKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEREDAP LPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYDELLHIKALSHLSTTVKREL AGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVL REDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAANQGNSQPQQKYTVMSGTPEKI LEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVI RLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMAAFKEQLPELEKIVKQISEDAKAPQKKHKVLL QQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGG EKVVLKSNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELF NCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEVCLCSQLSKRVQLLKKFIKIAAHCKEYK NLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLI KDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRT LSQMSHRLEPRRP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019688 |
ORF Size | 2979 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019688.2, NP_062662.1 |
RefSeq Size | 2982 bp |
RefSeq ORF | 2982 bp |
Locus ID | 56508 |
UniProt ID | Q9EQZ6 |
Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222997 | Rapgef4 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2, (10ug) |
CNY 12,830.00 |
|
MR222195 | Rapgef4 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 7,272.00 |
|
MR222195L3 | Lenti ORF clone of Rapgef4 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 10,550.00 |
|
MR222195L4 | Lenti ORF clone of Rapgef4 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 10,550.00 |
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