Rapgef4 (NM_019688) Mouse Untagged Clone

CAT#: MC222997

Rapgef4 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2, (10ug)



  "NM_019688" in other vectors (4)

CNY 12,830.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222997 representing NM_019688
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGCTGCGCACGCTGCACACTCTCAGTCCTCGGCCGAGTGGATCGCCTGCCTGGATAAAAGGCCGT
TGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATT
TCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACACTG
TTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTG
AGACCAGCAGTCACCAGGATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCAT
TCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAG
GAGGACTTCAAGGCACTATGGGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTG
TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAATGTCCCTTCAGAGAAGATCCTCAG
AGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCAC
CTAAAGACATACAGACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTG
TTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGA
CCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCT
TTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGA
TGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGA
GATTATCTACGACGAGCTCCTTCATATTAAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTA
GCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTA
CCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCT
GCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTT
CGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGG
CGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAG
AGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATT
TTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATG
ACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCA
CGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATC
CGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCC
TGGAGGAGTTCTATGTGTCTGTATCAGATGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGA
GCTGGAGAAGATTGTCAAGCAAATCTCAGAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTG
CAACAGTTCAACACAGGTGACGAGAGGGCCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGT
TCAAGGTCTACTGCATCGACCACACCTATACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGT
CATCAGTGCAGTAGCTGACAAACTGGGCTCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGA
GAAAAGGTGGTGCTGAAATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTG
CCTGCCCGAGAGAGCAATTCGACTCACTGACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGT
GGGAACATTTGAGCTGATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTC
AACTGTGTGCATGAGCTGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAA
ACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTC
CCAGCTCAGCAAACGTGTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAA
AATCTAAATTCCTTTTTCGCCATCGTCATGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGT
GGGAGAAACTGCCGAGCAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAA
CCACAGGGCATACAGGCTGACAGCAGCCAAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATT
AAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGC
GCATGATTGCAAACACTGCCAGAACAGTACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCA
AGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACT
TTATCACAGATGTCACACAGATTAGAGCCTCGAAGGCCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_019688
Insert Size 2982 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019688.2, NP_062662.1
RefSeq Size 4166 bp
RefSeq ORF 2982 bp
Locus ID 56508
UniProt ID Q9EQZ6
Gene Summary Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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