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Rapgef4 (NM_019688) Mouse Untagged Clone
CAT＃: MC222997
Rapgef4 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2, (10ug)
CNY 12,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222997 representing NM_019688
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCGCTGCGCACGCTGCACACTCTCAGTCCTCGGCCGAGTGGATCGCCTGCCTGGATAAAAGGCCGT TGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATT TCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACACTG TTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTG AGACCAGCAGTCACCAGGATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCAT TCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAG GAGGACTTCAAGGCACTATGGGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTG TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAATGTCCCTTCAGAGAAGATCCTCAG AGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCAC CTAAAGACATACAGACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTG TTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGA CCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCT TTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGA TGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGA GATTATCTACGACGAGCTCCTTCATATTAAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTA GCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTA CCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCT GCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTT CGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGG CGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAG AGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATT TTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATG ACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCA CGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATC CGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCC TGGAGGAGTTCTATGTGTCTGTATCAGATGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGA GCTGGAGAAGATTGTCAAGCAAATCTCAGAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTG CAACAGTTCAACACAGGTGACGAGAGGGCCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGT TCAAGGTCTACTGCATCGACCACACCTATACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGT CATCAGTGCAGTAGCTGACAAACTGGGCTCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGA GAAAAGGTGGTGCTGAAATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTG CCTGCCCGAGAGAGCAATTCGACTCACTGACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGT GGGAACATTTGAGCTGATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTC AACTGTGTGCATGAGCTGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAA ACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTC CCAGCTCAGCAAACGTGTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAA AATCTAAATTCCTTTTTCGCCATCGTCATGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGT GGGAGAAACTGCCGAGCAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAA CCACAGGGCATACAGGCTGACAGCAGCCAAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATT AAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGC GCATGATTGCAAACACTGCCAGAACAGTACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCA AGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACT TTATCACAGATGTCACACAGATTAGAGCCTCGAAGGCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019688 |
| Insert Size | 2982 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019688.2, NP_062662.1 |
| RefSeq Size | 4166 bp |
| RefSeq ORF | 2982 bp |
| Locus ID | 56508 |
| UniProt ID | Q9EQZ6 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222195 | Rapgef4 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), (10ug) |
CNY 9,500.00 |
|
| MR222195 | Rapgef4 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 7,272.00 |
|
| MR222195L3 | Lenti ORF clone of Rapgef4 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 10,550.00 |
|
| MR222195L4 | Lenti ORF clone of Rapgef4 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 2 |
CNY 10,550.00 |
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