Psmd4 (NM_008951) Mouse Tagged ORF Clone
CAT#: MG222032
- TrueORF®
Psmd4 (tGFP-tagged) - Mouse proteasome (prosome macropain) 26S subunit non-ATPase 4 (Psmd4), (10ug)
ORF Plasmid: DDK
"NM_008951" in other vectors (3)
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CNY 4,090.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Af1; angiocidin; Mcb1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222032 representing NM_008951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACAGTGAGTACATGCGGAACGGAGACTTCCTTCCCA CCCGGCTGCAGGCCCAGCAGGATGCCGTCAACATTGTATGTCACTCAAAGACCCGAAGCAACCCTGAGAA TAACGTGGGCCTGATCACACTGGCCAATGACTGTGAGGTGCTGACCACACTCACCCCGGACACTGGCCGT ATCCTCTCCAAGCTCCACACTGTCCAACCCAAAGGCAAGATCACCTTCTGCACTGGCATCCGCGTGGCCC ACTTGGCTCTGAAGCACCGGCAGGGCAAGAATCACAAGATGCGCATCATCGCCTTTGTCGGTAGCCCTGT GGAGGACAACGAGAAGGATCTGGTGAAACTAGCTAAACGCCTTAAGAAAGAAAAAGTGAATGTTGACATC ATTAATTTTGGGGAAGAGGAGGTGAACACAGAGAAGCTGACAGCCTTTGTGAACACACTGAATGGCAAGG ATGGAACTGGGTCCCATCTAGTGACAGTGCCTCCTGGACCTAGCTTGGCTGATGCTCTCATCAGTTCTCC TATTCTGGCTGGTGAAGGCGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAGTTTGGAGTAGATCCC AGTGCTGATCCTGAATTGGCCCTGGCCCTTCGAGTCTCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAAG AGGCACGGCGGGCCGCTGCGGCCTCTGCAGCTGAGGCTGGAATTGCTACACCTGGGACTGAAGACTCGGA TGACGCCCTACTGAAGATGACCATCAACCAGCAGGAGTTTGGCCGTCCTGGGCTTCCAGACCTAAGCAGC ATGACTGAGGAAGAGCAGATCGCCTACGCCATGCAGATGTCCCTGCAGGGAACAGAGTTTAGCCAAGAAT CGGCTGACATGGATGCCAGCTCAGCCATGGACACATCTGATCCAGTCAAGGAGGAGGATGACTATGACGT CATGCAGGACCCGGAGTTCCTTCAGAGCGTCCTAGAGAACCTTCCAGGTGTGGATCCCAACAATGCAGCC ATTCGAAGTGTCATGGGGGCTCTGGCCTCCCAGGCCACCAAGGATGGCAAGAATGACAAGAAAGAGGAAG AGAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222032 representing NM_008951
Red=Cloning site Green=Tags(s) MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGR ILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDI INFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDP SADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEDSDDALLKMTINQQEFGRPGLPDLSS MTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDVMQDPEFLQSVLENLPGVDPNNAA IRSVMGALASQATKDGKNDKKEEEKK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008951 |
ORF Size | 1128 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008951.2 |
RefSeq Size | 1252 bp |
RefSeq ORF | 1131 bp |
Locus ID | 19185 |
UniProt ID | O35226 |
Gene Summary | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR222032 | Psmd4 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (Psmd4) |
CNY 3,710.00 |
|
MR222032L3 | Lenti ORF clone of Psmd4 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (Psmd4) |
CNY 5,610.00 |
|
MR222032L4 | Lenti ORF clone of Psmd4 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (Psmd4) |
CNY 5,610.00 |