Gsx2 (NM_133256) Mouse Tagged ORF Clone

CAT#: MG221852

  • TrueORF®

Gsx2 (tGFP-tagged) - Mouse GS homeobox 2 (Gsx2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_133256" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Gsh-2; Gsh2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG221852 representing NM_133256
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGCTCCTTCTACGTGGACTCACTCATCATCAAGGACTCCTCACGGCCCGCACCCTCGCTGCCGG
AATCGCACCCAGGGCCGGATTTCTTCATCCCGCTGGGCATGCCGTCCCCGTTGGTGATGTCAGTGTCCGG
GCCTGGCTGTCCGTCCCGCAAAAGCGGCGCTTTCTGCGTGTGCCCGCTTTGCGTCACCTCGCACCTGCAC
TCCTCTCGCCCGCCCGCAGGAGCTGGTGGCGGAGCTACGGGGACCGCGGGGGCTGCAGTGGCGGGCGGCG
GGGTGGCTGGAGGCACCGGCGCTCTACCTTTGCTCAAAAGCCAGTTCTCTCCCGCTCCTGGGGACGCGCA
GTTTTGCCCACGGGTGAGCCACGCACACCATCACCACCACCCACCACAGCATCATCATCACCATCACCAG
CCCCAGCAGCCCGGCTCGGCTGCAGCGGCGGCCGCGGCGGCGGCGGCGGCAGCTGCGGCAGCGGCGGCTC
TGGGACACCCGCAGCACCACGCACCTGTCTGCGCCGCCACTACCTACAACATGTCGGACCCACGGAGATT
CCACTGCCTCTCCATGGGAGGCTCCGATACCAGCCAGGTACCCAATGGCAAAAGGATGAGGACAGCGTTT
ACCAGCACGCAGCTCCTGGAGCTGGAGCGAGAATTCTCTTCCAATATGTACCTGTCCCGACTCCGGAGAA
TCGAGATCGCGACATACCTAAACCTGTCAGAGAAGCAGGTGAAAATCTGGTTTCAGAACCGTCGCGTGAA
GCACAAGAAGGAGGGGAAAGGCGCTTCGAGGAACAATCACACAAGCTGCAAGTGCGTGGGTAGCCAGGCG
CATTATGCGCGCTCCGAGGACGAGGACTCCTTGTCCCCTGCCTCGGCTAACGAAGACAAGGAGATTTCCC
CCTTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG221852 representing NM_133256
Red=Cloning site Green=Tags(s)

MSRSFYVDSLIIKDSSRPAPSLPESHPGPDFFIPLGMPSPLVMSVSGPGCPSRKSGAFCVCPLCVTSHLH
SSRPPAGAGGGATGTAGAAVAGGGVAGGTGALPLLKSQFSPAPGDAQFCPRVSHAHHHHHPPQHHHHHHQ
PQQPGSAAAAAAAAAAAAAAAAALGHPQHHAPVCAATTYNMSDPRRFHCLSMGGSDTSQVPNGKRMRTAF
TSTQLLELEREFSSNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKGASRNNHTSCKCVGSQA
HYARSEDEDSLSPASANEDKEISPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133256
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133256.2, NP_573555.1
RefSeq Size 1665 bp
RefSeq ORF 918 bp
Locus ID 14843
UniProt ID P31316
Gene Summary During telencephalic development, causes ventralization of pallial progenitors and, depending on the developmental stage, specifies different neuronal fates. At early stages, necessary and sufficient to correctly specify the ventral lateral ganglionic eminence (LGE) and its major derivatives, the striatal projection neurons. At later stages, may specify LGE progenitors toward dorsal LGE fates, including olfactory bulb interneurons (PubMed:19709628). Transcription factor that binds 5'-CNAATTAG-3' DNA sequence (PubMed:7619729).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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