United States
Germany
Japan
United Kingdom
China
Sin3b (NM_009188) Mouse Tagged ORF Clone
CAT＃: MG220995
- TrueORF®
Sin3b (tGFP-tagged) - Mouse transcriptional regulator SIN3B (yeast) (Sin3b) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_009188" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810430C10Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220995 representing NM_009188
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCATGCAGGCAGCGGAGGCTCCGCGGGCCGGGGATTCGGTGGCTCCCGCTGGGGTCGTTCGGGCT CCGGAGGGCACGAGAAGCTGCCGGTGCACGTGGAGGACGCTCTCACCTACCTCGACCAGGTGAAGATCCG CTTTGGCAGTGACCCTGCCACCTACAACGGCTTCCTGGAGATCATGAAGGAGTTCAAAAGCCAGAGCATC GACACTCCTGGTGTGATCAGGCGTGTGTCCCAGCTCTTCCATGAGCACCCTGACCTCATCGTGGGGTTTA ATGCTTTCCTTCCGCTTGGATACCGTATAGACATTCCCAAGAACGGCAAGCTGAACATCCAGTCGCCGCT GAGCAGCCAGGACAACTCCCATAGCCATGGTGACTGTGGGGAGGACTTCAAGCAGATGTCCTACAAGGAG GACAGAGGCCAGGTGCCCCTGGAGTCTGACTCTGTGGAGTTCAACAATGCTATCAGCTATGTGAACAAGA TCAAGACCCGCTTTCTGGACCACCCTGAGATCTACAGGTCCTTCCTGGAGATCCTACACACCTACCAGAA GGAACAGCTGCACACTAAGGGCCGTCCATTCCGTGGCATGTCTGAGGAGGAGGTTTTCACAGAGGTGGCC AATCTTTTCCGTGGTCAGGAGGACCTGCTGTCAGAGTTCGGACAGTTCCTGCCTGAGGCAAAGCGGTCCC TGTTTACAGGCAATGGGTCGTGCGAGATGAACAGTGGGCAGAAGAATGAGGAGAAGAGTCTGGAACACAA CAAGAAACGCTCTCGGCCTTCACTCCTGCGCCCTGTGTCTGCGCCAGCCAAGAAGAAGATGAAGCTCCGA GGCACCAAAGACCTGTCCATTGCTGCCGTGGGGAAATACGGCACACTGCAGGAATTCTCCTTCTTTGACA AGGTGCGCAGGGTGCTGAAGAGCCAGGAGGTATATGAGAACTTTCTGCGCTGCATTGCGCTCTTCAATCA GGAGCTGGTGTCCGGGTCTGAGTTGCTGCAGCTTGTCAGTCCATTCCTGGGGAAATTTCCAGAACTCTTT GCACAGTTCAAGTCCTTCCTAGGAGTGAAAGAGCTGTCCTTTGCACCACCTATGAGTGACCGGTCTGGAG ATGGGATAAGCAGAGAAATTGACTATGCGTCTTGTAAGCGAATAGGATCCAGCTATCGGGCACTTCCCAA GACCTACCAGCAGCCCAAGTGCAGCGGAAGGACTGCCATTTGCAAGGAGGTCCTGAATGATACATGGGTC TCCTTTCCATCCTGGTCTGAAGACTCCACCTTTGTTAGCTCCAAGAAGACGCCCTACGAGGAGCAACTGC ATCGCTGTGAGGATGAGAGATTTGAATTGGATGTTGTCCTGGAGACCAACCTGGCCACCATCCGAGTGCT GGAGAGTGTGCAGAAGAAGTTGTCACGGATGGCTCCTGAGGACCAGGAGAAGCTCCGCCTGGATGACTGC CTGGGTGGCACATCAGAGGTGATTCAACGCCGTGCCATACACCGCATCTATGGAGACAAGGCCCCAGAGG TCATTGAGAGCCTGAAAAGGAACCCTGCCACCGCTGTGCCTGTCGTTCTGAAAAGGCTGAAGGCCAAGGA GGAGGAGTGGCGGGAGGCCCAGCAGGGCTTCAACAAGATCTGGCGTGAGCAGTACGAGAAAGCATACCTC AAGTCCCTGGACCACCAGGCTGTGAACTTCAAGCAGAATGACACCAAGGCACTGCGCTCTAAGAGCCTGC TCAATGAGATCGAAAGTGTCTATGATGAGCACCAGGAGCAGCACTCAGAGGGCCGCAGTGCGCCTTCCAG CGAGCCGCACCTTATCTTCGTGTATGAGGACCGACAGATCCTAGAGGACGCAGCAGCACTCATCAGCTAC TATGTTAAGCGGCAGCCAGCCATTCAGAAAGAGGACCAGGGCACTATCCGCCAGCTGCTTCACCGTTTCC TGCCCAGCCTGTTCTTCTCGCAGCAGTGCCCCGGCACCTCCGATGACTCTGCTGATGAGCGCGACCGGGA CCGGGACAGCGCTGAGCCTGAGCGCCGCCGGCCCACAGATGAGAAGCCCCCTGCAGATGCATCTCCAGAA CCGCCCAAGGTCCTGGACGATGTGTACAGTCTGTTTTTTGCCAACAACAACTGGTACTTCTTCCTGCGGC TGCACCAGACGCTGTGCGCCCGCCTGCTCAAGATCTACCGGCAGGCGCAGAAGCAGCTGCTGGAACATCG ACGAGAGCAGGAGAGGGAGCAGCTGCTGTGCGAGGGCCGCCGTGAGAAGGCTGCAGACCCTGCCATGGAG CTGCGACTCAAGCAGCCTAGTGAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGA GCCTGCTAGAGGGGAGCATCGACCCCACACAGTATGAGGACACTCTCCGTGAGATGTTCACCATCCATGC CTACATTGGCTTCACTATGGACAAGCTAGTGCAGAACATTGCCCGCCAGCTACACCATCTGGTGAGTGAC GACGTATGTCTGAAGGTGGTAGAGCTGTACCTGAATGAGCAGCAGCGAGGGGCAGCGGGGGGAAACCTGT CCTCCAGATGTGTTCGTGCAGCACGGGAGACTAGCTATCAGTGGAAGGCGGAGCGCTGCATGGCAGATGA GAACTGCTTCAAGGTAATGTTCCTGCAGCGCAGAGGTCAGGTGATCATGACCATAGAGCTCCTGGACACA GAAGAGGCACAGACTGAGGACCCTGTGGAGGTACAGCACCTGGCTCGCTATGTGGAGCAGTATGTGGGGT CTGAGGGTGCATCCAGCTCATCTACTGAAGGCTTCCTGCTGAAGCCGGTGTTCCTGCAGAGGAACCTGAA GAAGTTCCGGCGTTGGCAGTGTGAGCAGGTACGTGCCATGCGCGGTGAGGCTAAGAGCTCCTGGAAGCGG CTCATGGGCGTGGAGAGCGCTTGTGATGTGGACTGCCGGTTCCGCTTGGGCACACACAAGATGGTGTTCA TCGTCAATTCCGAGGACTACATGTACCGCCGTGGGACACTATGCCGTGCCAAGCAGGTGCAGCCCCTGGT GTTGTTGCGCCACCACCGGCACTTCGAGGAGTGGCATGGTCGCTGGCTGGAGGACAATGTAACTGTGGCG GCGGCAGGGCTGGTGCAGGACTGGCTGATGGGTGAGGAAGAGGAGGACATGGTACCCTGCAAGACGCTTT GCGAGACTGCGCATGTTCACGGGCTGCCGGTGACTCGCTACCGAGTGCAGTACAGCCGCCGCCCTGCTTC ACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220995 representing NM_009188
Red=Cloning site Green=Tags(s) MAHAGSGGSAGRGFGGSRWGRSGSGGHEKLPVHVEDALTYLDQVKIRFGSDPATYNGFLEIMKEFKSQSI DTPGVIRRVSQLFHEHPDLIVGFNAFLPLGYRIDIPKNGKLNIQSPLSSQDNSHSHGDCGEDFKQMSYKE DRGQVPLESDSVEFNNAISYVNKIKTRFLDHPEIYRSFLEILHTYQKEQLHTKGRPFRGMSEEEVFTEVA NLFRGQEDLLSEFGQFLPEAKRSLFTGNGSCEMNSGQKNEEKSLEHNKKRSRPSLLRPVSAPAKKKMKLR GTKDLSIAAVGKYGTLQEFSFFDKVRRVLKSQEVYENFLRCIALFNQELVSGSELLQLVSPFLGKFPELF AQFKSFLGVKELSFAPPMSDRSGDGISREIDYASCKRIGSSYRALPKTYQQPKCSGRTAICKEVLNDTWV SFPSWSEDSTFVSSKKTPYEEQLHRCEDERFELDVVLETNLATIRVLESVQKKLSRMAPEDQEKLRLDDC LGGTSEVIQRRAIHRIYGDKAPEVIESLKRNPATAVPVVLKRLKAKEEEWREAQQGFNKIWREQYEKAYL KSLDHQAVNFKQNDTKALRSKSLLNEIESVYDEHQEQHSEGRSAPSSEPHLIFVYEDRQILEDAAALISY YVKRQPAIQKEDQGTIRQLLHRFLPSLFFSQQCPGTSDDSADERDRDRDSAEPERRRPTDEKPPADASPE PPKVLDDVYSLFFANNNWYFFLRLHQTLCARLLKIYRQAQKQLLEHRREQEREQLLCEGRREKAADPAME LRLKQPSEVELEEYYPAFLDMVRSLLEGSIDPTQYEDTLREMFTIHAYIGFTMDKLVQNIARQLHHLVSD DVCLKVVELYLNEQQRGAAGGNLSSRCVRAARETSYQWKAERCMADENCFKVMFLQRRGQVIMTIELLDT EEAQTEDPVEVQHLARYVEQYVGSEGASSSSTEGFLLKPVFLQRNLKKFRRWQCEQVRAMRGEAKSSWKR LMGVESACDVDCRFRLGTHKMVFIVNSEDYMYRRGTLCRAKQVQPLVLLRHHRHFEEWHGRWLEDNVTVA AAGLVQDWLMGEEEEDMVPCKTLCETAHVHGLPVTRYRVQYSRRPASP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009188 |
| ORF Size | 3294 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009188.4, NP_033214.2 |
| RefSeq Size | 4117 bp |
| RefSeq ORF | 3297 bp |
| Locus ID | 20467 |
| UniProt ID | Q62141 |
| Gene Summary | Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223551 | Sin3b (untagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1, (10ug) |
CNY 14,060.00 |
|
| MR220995 | Sin3b (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1 |
CNY 12,232.00 |
|
| MR220995L1 | Lenti ORF clone of Sin3b (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1 |
CNY 10,260.00 |
|
| MR220995L2 | Lenti ORF clone of Sin3b (mGFP-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1 |
CNY 14,632.00 |
|
| MR220995L3 | Lenti ORF clone of Sin3b (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1 |
CNY 10,260.00 |
|
| MR220995L4 | Lenti ORF clone of Sin3b (mGFP-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1 |
CNY 10,260.00 |
