Rgr (NM_021340) Mouse Tagged ORF Clone
CAT#: MG220572
- TrueORF®
Rgr (tGFP-tagged) - Mouse retinal G protein coupled receptor (Rgr), (10ug)
ORF Plasmid: DDK
"NM_021340" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220572 representing NM_021340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAACCAGGGCCCTGCCTGCTGGTTTGGGGGAACTTGAGGTGCTGGCTGTAGGAACAGTCCTGC TGATGGAAGCGCTCTCTGGCATCAGCCTCAATGGCCTGACCATCTTCTCTTTCTGCAAGACTCCAGATCT GCGGACTCCCAGCAACCTGCTGGTACTGAGCCTGGCCCTGGCAGACACTGGGATCAGCCTGAATGCCCTT GTTGCAGCGGTATCCAGCCTCCTCCGGCGCTGGCCACATGGTTCAGAGGGCTGCCAGGTTCATGGTTTCC AGGGATTTGCAACAGCACTGGCCAGCATCTGTGGCAGTGCAGCCGTTGCCTGGGGACGTTATCACCACTA CTGCACCCGCAGACAGTTGGCATGGGACACGGCCATCCCTCTGGTGCTGTTTGTGTGGATGTCATCTGCC TTCTGGGCATCCCTGCCCCTGATGGGCTGGGGCCACTATGACTATGAGCCTGTGGGGACATGCTGTACAC TGGACTATTCGAGGGGTGACAGAAACTTCATCAGTTTTCTCTTCACCATGGCTTTTTTCAACTTCCTCGT ACCCCTGTTCATCACACACACTTCATACCGGTTCATGGAGCAGAAATTCTCCAGGAGTGGCCATCTCCCG GTGAATACTACTCTTCCAGGCAGAATGCTGCTGCTTGGCTGGGGCCCCTATGCCCTCCTGTACCTATACG CAGCCATCGCAGATGTGAGCTTCATCTCTCCTAAACTACAGATGGTGCCTGCTCTCATCGCCAAAACCAT GCCCACAATCAACGCCATCAACTATGCGTTGCACAGGGAGATGGTCTGTAGAGGAACCTGGCAGTGTCTG TCTCCACAAAAAAGCAAGAAGGACCGAACCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220572 representing NM_021340
Red=Cloning site Green=Tags(s) MAATRALPAGLGELEVLAVGTVLLMEALSGISLNGLTIFSFCKTPDLRTPSNLLVLSLALADTGISLNAL VAAVSSLLRRWPHGSEGCQVHGFQGFATALASICGSAAVAWGRYHHYCTRRQLAWDTAIPLVLFVWMSSA FWASLPLMGWGHYDYEPVGTCCTLDYSRGDRNFISFLFTMAFFNFLVPLFITHTSYRFMEQKFSRSGHLP VNTTLPGRMLLLGWGPYALLYLYAAIADVSFISPKLQMVPALIAKTMPTINAINYALHREMVCRGTWQCL SPQKSKKDRTQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021340 |
ORF Size | 873 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021340.4 |
RefSeq Size | 1659 bp |
RefSeq ORF | 876 bp |
Locus ID | 57811 |
UniProt ID | Q9Z2B3 |
Gene Summary | The gene is a member of the opsin family of G-protein coupled receptors. The encoded protein is expressed in the retina, and acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. Disruption of a similar gene in human is associated with autosomal recessive (arRP) and autosomal dominant retinitis pigmentosa (adRP). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210154 | Rgr (untagged) - Mouse retinal G protein coupled receptor (Rgr), (10ug) |
CNY 3,600.00 |
|
MR220572 | Rgr (Myc-DDK-tagged) - Mouse retinal G protein coupled receptor (Rgr) |
CNY 3,600.00 |
|
MR220572L3 | Lenti ORF clone of Rgr (Myc-DDK-tagged) - Mouse retinal G protein coupled receptor (Rgr) |
CNY 5,890.00 |
|
MR220572L4 | Lenti ORF clone of Rgr (mGFP-tagged) - Mouse retinal G protein coupled receptor (Rgr) |
CNY 5,890.00 |