Rgr (NM_021340) Mouse Untagged Clone

CAT#: MC210154

Rgr (untagged) - Mouse retinal G protein coupled receptor (Rgr), (10ug)



  "NM_021340" in other vectors (4)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
RGR Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210154 representing NM_021340
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAACCAGGGCCCTGCCTGCTGGTTTGGGGGAACTTGAGGTGCTGGCTGTAGGAACAGTCCTGC
TGATGGAAGCGCTCTCTGGCATCAGCCTCAATGGCCTGACCATCTTCTCTTTCTGCAAGACTCCAGATCT
GCGGACTCCCAGCAACCTGCTGGTACTGAGCCTGGCCCTGGCAGACACTGGGATCAGCCTGAATGCCCTT
GTTGCAGCGGTATCCAGCCTCCTCCGGCGCTGGCCACATGGTTCAGAGGGCTGCCAGGTTCATGGTTTCC
AGGGATTTGCAACAGCACTGGCCAGCATCTGTGGCAGTGCAGCCGTTGCCTGGGGACGTTATCACCACTA
CTGCACCCGCAGACAGTTGGCATGGGACACGGCCATCCCTCTGGTGCTGTTTGTGTGGATGTCATCTGCC
TTCTGGGCATCCCTGCCCCTGATGGGCTGGGGCCACTATGACTATGAGCCTGTGGGGACATGCTGTACAC
TGGACTATTCGAGGGGTGACAGAAACTTCATCAGTTTTCTCTTCACCATGGCTTTTTTCAACTTCCTCGT
ACCCCTGTTCATCACACACACTTCATACCGGTTCATGGAGCAGAAATTCTCCAGGAGTGGCCATCTCCCG
GTGAATACTACTCTTCCAGGCAGAATGCTGCTGCTTGGCTGGGGCCCCTATGCCCTCCTGTACCTATACG
CAGCCATCGCAGATGTGAGCTTCATCTCTCCTAAACTACAGATGGTGCCTGCTCTCATCGCCAAAACCAT
GCCCACAATCAACGCCATCAACTATGCGTTGCACAGGGAGATGGTCTGTAGAGGAACCTGGCAGTGTCTG
TCTCCACAAAAAAGCAAGAAGGACCGAACCCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_021340
Insert Size 876 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC119055, AAI19056
RefSeq Size 4307 bp
RefSeq ORF 876 bp
Locus ID 57811
UniProt ID Q9Z2B3
Gene Summary The gene is a member of the opsin family of G-protein coupled receptors. The encoded protein is expressed in the retina, and acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. Disruption of a similar gene in human is associated with autosomal recessive (arRP) and autosomal dominant retinitis pigmentosa (adRP). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: removed 2 bases from the 5' end that did not align to the reference genome assembly.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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