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Plch1 (NM_001177732) Mouse Tagged ORF Clone
CAT＃: MG220515
- TrueORF®
Plch1 (tGFP-tagged) - Mouse phospholipase C eta 1 (Plch1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001177732" in other vectors (4)
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CNY 11,020.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BC042549; PLC-eta-1; PLCeta1; Plcl3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220515 representing NM_001177732
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTAATGCAGT CCGGGACACAGATGATCAAACTGAAGCGTGGCACCAAAGGGCTCGTTCGCCTCTTTTACCTGGATGAGCA CCGGACCCGCCTCCGATGGCGACCCTCTCGGAAGAGTGAGAAGGCAAAAATACTTATTGACTCCATCTAC AAAGTCACCGAGGGCAGGCAGTCTGAAATATTCCACAGACAGGCTGAGGGGAACTTTGACCCTAGCTGCT GCTTTACCATCTACCATGGCAACCACATGGAGTCTCTGGACCTCATTACCTCCAACCCAGAGGAGGCACG CACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAGAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAACGGTGATGGCTTATTGAATA TTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCCCGCAGAAAAGTCAGGCAAATGTT TCAGGAAGCAGATACAGATGAGAATCAGGGAACCTTGACATTTGAAGAGTTCTGCGTTTTTTACAAAATG ATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAAGACCACCTGACTGTGG AGGAGCTGGCTCAGTTTTTGAAAGTGGAACAAAAGATGAGTAATGTGACACTGGACTATTGTCTTGACAT CATAATGAAGTTTGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTTGGTATAGAAGGCTTCACGAAC TTCATGCGTAGCCCTGCCTGTGACGTATTTAACCCGTTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTGTGCAACTACTACATTGCTTCATCTCACAACACATACCTGACTGGGGATCAGCTCCTTTCTCAATC TAAAGTGGATATGTATGCACGGGTGCTACAAGAGGGTTGTAGATGCGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCATTCGTGAAGAATGAGTTCCCGGTTATCCTGTCCATTGAGAACCACTGCAG CATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGTATATTGCAGGACAAACTGGACCTGTCTTCT GTAGACACCGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAAATCCTAGTGAAGGGCA AGAAGTTGCCGTATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATGAGGACAGTGCAGATGA GATTGAAGATGAGTGCAAGTTCAAGCTGCATTACAGTAACGGGACCACTGAGCACCAGGTAGAATCTTTC ATACGGAAAAAGCTGGAGTCACTGTTGAAGGAGTCTCAGATTCGAGACAAAGAAGATCCAGACAGTTTCA CAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGCAGAACCTGGATGTAAA GGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACACAAGCAGAAAGCTACG AAATCACGTTCTAAATCCTATAGTACTGATGATGAGGATGATAGCCTTCAGAATCCTGGCAAGGAGGGAG GCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCTCGGACTTGGTGGT GTACACAAACTCCGTGGCAGCCCAGGATATTGTTGATGATGGAACCACAGGGAATGTGTTGTCCTTCAGC GAAACAAGAGCCCATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAACCAAAAACAGCTCA CAAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCACTGCCCTACTGGAATGCAGG CTGCCAGCTCGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATCAATCGAGCAAAATTCAAG GCGAATGGCAACTGTGGCTATATTCTCAAGCCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTG GTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGACAGCAACTCCCCAA ACCTCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTCGTTGAAGTTGAAATTATTGGG CTGCCAGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAACGGATTTAACCCTGTGTGGGAAG AAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCATGATCC TATTGGAAGAGACTTTGTCGGACAAAGGACTGTGACCTTCAGTAGCCTCGTGCCCGGCTACCGGCATGTC TACCTGGAAGGACTCACAGAAGCATCTATTTTCGTTCACATAACCATCAATGAGATCTTTGGAAAGTGGA GTCCTTTAATCCTCAACCCCAGTTATACCATATTGCACTTTCTAGGAGCTACAAAGAACAGACAGCTCCA AGGCCTGAAGGGGCTGTTCAATAAGAATCCCAGGCACGCTTCTTCAGAAAACAACTCGCATTATGTTCGG AAGCGATCGATTGGAGATAGGATTCTGCGACGTACAGCTAGTGCTCCAGCCAAAGGCAGGAAAAAGAGCA AAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTCTGAGGCTTCAAGAGATCAAGATGGTGT CCTGAGGAGGACCACCCGGAGTCTGCAAGTACGCCCTGTCTCTATGCCTGTTGACAAGAGCCTTCTGGGG GCTTTGTCGCTGCCCATATCTGAAGCAGCAAAAGACACGGATGGAAAAGAAAACTGTCTGGTACAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220515 representing NM_001177732
Red=Cloning site Green=Tags(s) MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIY KVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQ RTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKM MSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTN FMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDG PDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSS VDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESF IRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKAT KSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFS ETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFK ANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIG LPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHV YLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVR KRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLG ALSLPISEAAKDTDGKENCLVQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177732 |
| ORF Size | 3009 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177732.2 |
| RefSeq Size | 6294 bp |
| RefSeq ORF | 3012 bp |
| Locus ID | 269437 |
| UniProt ID | Q4KWH5 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223161 | Plch1 (untagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3, (10ug) |
CNY 12,920.00 |
|
| MR220515 | Plch1 (Myc-DDK-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 9,980.00 |
|
| MR220515L3 | Lenti ORF clone of Plch1 (Myc-DDK-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 11,880.00 |
|
| MR220515L4 | Lenti ORF clone of Plch1 (mGFP-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 11,880.00 |
