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Plch1 (NM_001177732) Mouse Untagged Clone
CAT＃: MC223161
Plch1 (untagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3, (10ug)
CNY 12,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BC042549; PLC-eta-1; PLCeta1; Plcl3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223161 representing NM_001177732
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTAATGCAGT CCGGGACACAGATGATCAAACTGAAGCGTGGCACCAAAGGGCTCGTTCGCCTCTTTTACCTGGATGAGCA CCGGACCCGCCTCCGATGGCGACCCTCTCGGAAGAGTGAGAAGGCAAAAATACTTATTGACTCCATCTAC AAAGTCACCGAGGGCAGGCAGTCTGAAATATTCCACAGACAGGCTGAGGGGAACTTTGACCCTAGCTGCT GCTTTACCATCTACCATGGCAACCACATGGAGTCTCTGGACCTCATTACCTCCAACCCAGAGGAGGCACG CACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAGAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAACGGTGATGGCTTATTGAATA TTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCCCGCAGAAAAGTCAGGCAAATGTT TCAGGAAGCAGATACAGATGAGAATCAGGGAACCTTGACATTTGAAGAGTTCTGCGTTTTTTACAAAATG ATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAAGACCACCTGACTGTGG AGGAGCTGGCTCAGTTTTTGAAAGTGGAACAAAAGATGAGTAATGTGACACTGGACTATTGTCTTGACAT CATAATGAAGTTTGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTTGGTATAGAAGGCTTCACGAAC TTCATGCGTAGCCCTGCCTGTGACGTATTTAACCCGTTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTGTGCAACTACTACATTGCTTCATCTCACAACACATACCTGACTGGGGATCAGCTCCTTTCTCAATC TAAAGTGGATATGTATGCACGGGTGCTACAAGAGGGTTGTAGATGCGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCATTCGTGAAGAATGAGTTCCCGGTTATCCTGTCCATTGAGAACCACTGCAG CATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGTATATTGCAGGACAAACTGGACCTGTCTTCT GTAGACACCGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAAATCCTAGTGAAGGGCA AGAAGTTGCCGTATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATGAGGACAGTGCAGATGA GATTGAAGATGAGTGCAAGTTCAAGCTGCATTACAGTAACGGGACCACTGAGCACCAGGTAGAATCTTTC ATACGGAAAAAGCTGGAGTCACTGTTGAAGGAGTCTCAGATTCGAGACAAAGAAGATCCAGACAGTTTCA CAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGCAGAACCTGGATGTAAA GGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACACAAGCAGAAAGCTACG AAATCACGTTCTAAATCCTATAGTACTGATGATGAGGATGATAGCCTTCAGAATCCTGGCAAGGAGGGAG GCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCTCGGACTTGGTGGT GTACACAAACTCCGTGGCAGCCCAGGATATTGTTGATGATGGAACCACAGGGAATGTGTTGTCCTTCAGC GAAACAAGAGCCCATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAACCAAAAACAGCTCA CAAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCACTGCCCTACTGGAATGCAGG CTGCCAGCTCGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATCAATCGAGCAAAATTCAAG GCGAATGGCAACTGTGGCTATATTCTCAAGCCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTG GTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGACAGCAACTCCCCAA ACCTCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTCGTTGAAGTTGAAATTATTGGG CTGCCAGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAACGGATTTAACCCTGTGTGGGAAG AAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCATGATCC TATTGGAAGAGACTTTGTCGGACAAAGGACTGTGACCTTCAGTAGCCTCGTGCCCGGCTACCGGCATGTC TACCTGGAAGGACTCACAGAAGCATCTATTTTCGTTCACATAACCATCAATGAGATCTTTGGAAAGTGGA GTCCTTTAATCCTCAACCCCAGTTATACCATATTGCACTTTCTAGGAGCTACAAAGAACAGACAGCTCCA AGGCCTGAAGGGGCTGTTCAATAAGAATCCCAGGCACGCTTCTTCAGAAAACAACTCGCATTATGTTCGG AAGCGATCGATTGGAGATAGGATTCTGCGACGTACAGCTAGTGCTCCAGCCAAAGGCAGGAAAAAGAGCA AAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTCTGAGGCTTCAAGAGATCAAGATGGTGT CCTGAGGAGGACCACCCGGAGTCTGCAAGTACGCCCTGTCTCTATGCCTGTTGACAAGAGCCTTCTGGGG GCTTTGTCGCTGCCCATATCTGAAGCAGCAAAAGACACGGATGGAAAAGAAAACTGTCTGGTACAGATCT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177732 |
| Insert Size | 3012 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177732.1, NP_001171203.1 |
| RefSeq Size | 6294 bp |
| RefSeq ORF | 3012 bp |
| Locus ID | 269437 |
| UniProt ID | Q4KWH5 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3, also known as PLC-eta1a) differs in the 3' UTR and includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant 1. This results in an isoform (3, also known as phospholipase C-eta1a) that has a shorter and distinct C-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220515 | Plch1 (tGFP-tagged) - Mouse phospholipase C eta 1 (Plch1) transcript variant 3, (10ug) |
CNY 11,020.00 |
|
| MR220515 | Plch1 (Myc-DDK-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 9,980.00 |
|
| MR220515L3 | Lenti ORF clone of Plch1 (Myc-DDK-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 11,880.00 |
|
| MR220515L4 | Lenti ORF clone of Plch1 (mGFP-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 3 |
CNY 11,880.00 |
