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Ptpa (NM_138748) Mouse Tagged ORF Clone
CAT＃: MG219967
- TrueORF®
Ppp2r4 (tGFP-tagged) - Mouse protein phosphatase 2A regulatory subunit B (PR 53) (Ppp2r4), (10ug)
ORF Plasmid: DDK
"NM_138748" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610042B21Rik; C77440; N28142; Ppp2r4; PR53 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219967 representing NM_138748
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAAGAGACCCCTCCAACTACTCAGAACTTTA TCATTCCAAAAAAGGAGATCCACACAGTTCCAGATATGGGCAAATGGAAGCGCTCTCAGGCATATGCTGA CTACATTGGCTTCATCCTTACCCTCAATGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGACTACAAAGTC TCTGAGGCCATCGAGAAGCTGGTGGCACTTCTTGATACGCTGGATAGGTGGATTGATGAAACCCCGCCAG TGGACCAGCCTTCCCGGTTTGGGAACAAAGCCTACAGAACCTGGTATGCCAAACTTGATCAGGAAGCAGA AAACTTGGTGGCCACAGTGGTCCCCACCCACCTGGCTGCTGCTGTGCCTGAAGTGGCAGTTTACCTGAAG GAGGCTGTGGGGAACTCCACACGAATTGACTATGGCACAGGGCATGAGGCTGCCTTTGCTGCTTTCCTCT GTTGTCTCTGCAAGATTGGTGTACTCCGGGTGGACGACCAGGTGGCTATTGTCTTCAAGGTGTTTGATAG GTATCTTGAGGTTATGCGGAAGTTGCAGAAGACATACAGGATGGAGCCTGCAGGCAGCCAGGGCGTATGG GGTCTGGATGACTTCCAGTTCCTGCCCTTCATCTGGGGCAGCTCACAGCTCATAGACCACCCCCACCTGG AGCCCAGACATTTCGTGGATGAGAAGGCGGTGAGCGAGAACCACAAGGACTACATGTTTCTCCAGTGCAT CCTGTTCATCACTGAGATGAAGACTGGCCCCTTTGCGGAACACTCCAACCAGCTGTGGAACATCAGTGCT GTCCCCTCCTGGTCTAAAGTGAACCAGGGCCTCATTCGAATGTATAAGGCAGAGTGCCTGGAGAAGTTCC CTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCCGTCACATCAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219967 representing NM_138748
Red=Cloning site Green=Tags(s) MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFDYKV SEAIEKLVALLDTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLK EAVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVW GLDDFQFLPFIWGSSQLIDHPHLEPRHFVDEKAVSENHKDYMFLQCILFITEMKTGPFAEHSNQLWNISA VPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138748 |
| ORF Size | 969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138748.5, NP_620087.3 |
| RefSeq Size | 2577 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 110854 |
| UniProt ID | P58389 |
| Gene Summary | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202081 | Ppp2r4 (untagged) - Mouse protein phosphatase 2A, regulatory subunit B (PR 53) (Ppp2r4), (10ug) |
CNY 2,400.00 |
|
| MR219967 | Ppp2r4 (Myc-DDK-tagged) - Mouse protein phosphatase 2A, regulatory subunit B (PR 53) (Ppp2r4) |
CNY 2,400.00 |
|
| MR219967L3 | Lenti ORF clone of Ppp2r4 (Myc-DDK-tagged) - Mouse protein phosphatase 2A, regulatory subunit B (PR 53) (Ppp2r4) |
CNY 4,750.00 |
|
| MR219967L4 | Lenti ORF clone of Ppp2r4 (mGFP-tagged) - Mouse protein phosphatase 2A, regulatory subunit B (PR 53) (Ppp2r4) |
CNY 4,750.00 |
