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Rgs20 (NM_001177795) Mouse Tagged ORF Clone
CAT＃: MG219775
- TrueORF®
Rgs20 (tGFP-tagged) - Mouse regulator of G-protein signaling 20 (Rgs20) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001177795" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900073E09Rik; Rgsz1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219775 representing NM_001177795
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGCCTTTCCCAAGAAAACCAAGGGTACCTAGAAGAGCATCTTACCAGGCAGGCTGCACAGGCAC AGTTGCTGCCCCTCTTCAGAGCAGAGAAAGATTATAATCCCAGTATTGACCAAGACACAGAAGATAAAGA GGGATCCCAAGCCGTGACTGGTGTCAAGCTCCCAGACTCCACCGGGTCGACTCATTTCGCCACCTTTCTA GCCTGCTCTCCAGCTGTTCTTGTGTCTTCTGCGGCGACCCCCTCCCGTGGCTTGCCTGGGCGCAGGGACT TTCCCTCTCAGCCAAGCTCCTGGCCTACTGCCTGGCTCTCTCCAGAGCCCCACCGCCTCCTGTTCCTACT TGTGGCCTCAATGGCACTGCCAGGACGACGTCCGAGGGGTCGTCTACTGAAGCTGGCAAGTCCGAATTCG GAGCCCAGAGGAGAGGACGCCTGTACTGAAAAGAGTTCACCCGAGTCCCCTGAGGGATCTGAGCGGACGG AAATGCGGATGCGGCAGATGTGCGGCGGAAGTGAGACCCAGGGCCCTGCCCCAAGCCAGCAGGGTGGCCG GGGCTCCAACGCCTGCTGCTTCTGTTGGTGCTGCTGCTGCACCTGTTCTTGCCTCACTGTCAGAAACCAA GAAGACCAGAGACCCCAAAGAGCCTCCCATGAAATCAGAACAGACATTCCAGCATGTGAAGAAAGCCCCA CGCCTACTCTGGAAGAAGTCTGTGCCTGGGCCCAGTCTTTTGATAACCTGATGGTCACTCCAGCCGGAAG AAATGCATTCCGGGAATTTCTTCGAACAGAATTCAGTGAAGAAAACATGCTCTTCTGGATGGCCTGTGAA GAACTGAAAAGGGAAGCTAATAAAAGCACTATTGAGGAGAAAGCAAGGATAATCTATGAGGACTATATTT CTATTCTCTCTCCTAAAGAGGTCAGCTTGGACTCCCGAGTACGAGAGGTTATCAACAGAAACATGGTGGA TCCATCCCAGCACATATTCGATGACGCTCAACTGCAGATCTACACTCTAATGCACAGAGACTCCTATCCC CGCTTCATGAACTCCACAGTCTATAAGGATTTGCTTACGTCCTTAGCTGAGAAAACAGTTGAAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219775 representing NM_001177795
Red=Cloning site Green=Tags(s) MPSLSQENQGYLEEHLTRQAAQAQLLPLFRAEKDYNPSIDQDTEDKEGSQAVTGVKLPDSTGSTHFATFL ACSPAVLVSSAATPSRGLPGRRDFPSQPSSWPTAWLSPEPHRLLFLLVASMALPGRRPRGRLLKLASPNS EPRGEDACTEKSSPESPEGSERTEMRMRQMCGGSETQGPAPSQQGGRGSNACCFCWCCCCTCSCLTVRNQ EDQRPQRASHEIRTDIPACEESPTPTLEEVCAWAQSFDNLMVTPAGRNAFREFLRTEFSEENMLFWMACE ELKREANKSTIEEKARIIYEDYISILSPKEVSLDSRVREVINRNMVDPSQHIFDDAQLQIYTLMHRDSYP RFMNSTVYKDLLTSLAEKTVEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177795 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177795.1, NP_001171266.1 |
| RefSeq Size | 2125 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 58175 |
| Gene Summary | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210157 | Rgs20 (untagged) - Mouse regulator of G-protein signaling 20 (Rgs20), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR219775 | Rgs20 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 20 (Rgs20), transcript variant 1 |
CNY 3,656.00 |
|
| MR219775L3 | Lenti ORF clone of Rgs20 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 20 (Rgs20), transcript variant 1 |
CNY 5,230.00 |
|
| MR219775L4 | Lenti ORF clone of Rgs20 (mGFP-tagged) - Mouse regulator of G-protein signaling 20 (Rgs20), transcript variant 1 |
CNY 5,230.00 |
