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Ano2 (NM_153589) Mouse Tagged ORF Clone
CAT＃: MG219751
- TrueORF®
Ano2 (tGFP-tagged) - Mouse anoctamin 2 (Ano2), (10ug)
ORF Plasmid: DDK
"NM_153589" in other vectors (4)
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CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BC033409; Tmem16b |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219751 representing NM_153589
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCCTGGGCTGCGAGACATCCCTCTGCTCCCTGGATCTCCACGTCGGCTGAGTTCTCGGACCG TGGCCAGAGGAAGCCAGGGCCCCAAACATGGGCAGCAGTACCTCAAAGTGCCAGGTCACCGGGCCCCAGG TCAGAGGGACAATTCCAGCCTACACCCTAGCCAGGTCTCCAGGAGAGAGAGTTCCCGAGACCGTTCTGTC ATCAATAACTACCTGGATGCCAATGAGCCCCCGTCCTCGGAAGCCCGTCTGAGCCGCATGCACTTTCACG ACAACCAGAGGAAAGTCGACTATGTACTTGCATACCACTACCGGAAACGAGGCGCACACCTGGGTCACGG CTCCCCTGGACACTCTCTGGCTGTTATCTCCAATGGGGAGACAGGCAAGGAGCGCCATGGCGGGGGCCCT GGTGATGTTGAACTTGGGCCACTGGATGCTCTGGAAGAGGAAAGGAGGGAGCAGCGAGATGAATTTGAGC ACAACCTGATGGCTGCTGGGCTGGAGCTGGAGAAGGACTTGGAGAGCAAAAGCCAGGGATCTGTCTTTGT CCGGATCCACGCCCCATGGCAGGTGCTGGCCAGGGAAGCAGAGTTCTTGAAGATCAAAGTCCCCACCAAG AAAATGTACGAGATCAAAGCAGGAGGCAGCATCGCAAAGAAGTTCAGCGCCATTCTGCAGACGCTCAGTT CACCCCTGCAGCCCAGAGTTCCTGAACACAGCAACAACAGGATGAAAAACCTCTCCTACCCATTCTCCAG GGAAAAAATGTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGACAATGCTACCCGGAGCCGCATT GTACACGAGATTCTGAAGCGGACGGCCTGTTCTCGAGCCAACAACACGATGGGTATTAACTCTCTAATAG CAAACAATATCTACGAGGCTGCCTACCCGCTTCATGATGGTGAATATGACAGTCCAGGGGATGACATGAA TGACAGAAAGCTGCTGTATCAGGAATGGGCACGCTATGGAGTGTTTTACAAATTTCAACCCATTGACCTC ATCAGGAAGTATTTTGGAGAGAAAATTGGGCTATATTTTGCTTGGCTGGGGTTGTATACATCATTCCTGA TCCCATCTTCTGTCATAGGCGTGATTGTGTTTCTTTATGGATGTGCAACAATCGAGGAAGATATTCCCAG CAAAGAGATGTGTGACCACCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGACTATTGG AACCTCAGCTCAGCCTGTGGGACTGCACGGGCCAGTCATCTGTTTGACAATCCAGCCACCGTCTTCTTTT CCATCTTCATGGCTCTGTGGGCTACCATGTTTCTGGAAAACTGGAAGAGACTCCAGATGCGGTTGGGCTA CTTCTGGGACCTGACTGGGATCGAAGAGGAAGAAGAACGTTCCCAGGAACACTCTCGGCCTGAATATGAA ACCAAAGTACGAGAGAAACTGTTAAAGGAGAGTGGCAAGTCAGCGGTTCAGAAGCTGGAAGCAAATTCAC CTGAGGATGATGAGGATGATGAAGATAAACTGACTTGGAAAGATCGTTTCCCAGGATACTTGATGAACTT TGCCTCCATCTTGTTTATGATTGCCCTGACGTTCTCCATTGTCTTTGGGGTCATTGTGTATCGGATCACA ACTGCAGCTGCCCTGTCTCTCAACAAGGCCACACGCTCCAATGTCCGGGTGACAGTGACTGCAACAGCAG TCATCATCAACCTCGTGGTCATCCTCATCTTGGATGAGATCTATGGTGCTGTGGCCAAGTGGCTTACCAA AATCGAGGTTCCGAAAACAGAACAGACTTTTGAAGAACGGTTGATCCTCAAAGCTTTCTTGCTCAAGTTT GTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAGGGGAGGTTTGTGGGCAGACCTGGAAGCT ATGTCTACGTGTTCGACGGTTACCGCATGGAAGAGTGTGCTCCGGGAGGCTGTCTCATGGAGCTGTGCAT CCAACTCAGCATCATCATGTTGGGCAAGCAGCTGATCCAGAACAATATCTTTGAGATTGGAGTCCCGAAG CTAAAGAAACTCTTTCGGAAGCTGAAAGATGAGACAGAGCCTGGAGAAAGTGACCCTGACCACTCAAAAC GTCCAGAGCAGTGGGACCTGGACCACAGCCTAGAGCCTTACACTGGGCTGACTCCTGAGTACATGGAAAT GATCATCCAGTTTGGTTTTGTCACCCTTTTTGTGGCCTCCTTTCCTCTGGCCCCTGTGTTTGCTCTCCTC AACAATGTCATTGAAGTGCGGCTGGATGCAAAAAAGTTTGTTACAGAACTGAGACGCCCAGATGCTGTGA GGACCAAAGACATTGGAATTTGGTTTGACATTCTCTCTGGAATTGGCAAGTTCTCCGTCATCATTAATGC TTTTGTCATTGCTGTCACCTCCGACTTTATCCCCCGCCTTGTGTACCAGTACTCCTATAGCCACAACGGG ACACTACATGGCTTTGTGAACCATACACTCTCCTTTTTCAACGTCAGTCAGCTGAAGGAGGGAACACAGC CTGAAAACTCACAGTTCGACCAGGAGGTTCAGTTTTGCAGGTTCAAAGATTATCGAGAACCACCATGGGC ACCAAACCCTTATGAGTTTTCCAAGCAGTACTGGTCTGTTTTGTCTGCCCGTCTGGCCTTTGTTATCATC TTCCAGAACCTAGTGATGTTCCTGAGTGTCCTGGTGGACTGGATGATTCCGGATATCCCCACTGACATCA GCGACCAGATCAAGAAAGAGAAGAGCTTGCTGGTAGACTTCTTTCTGAAGGAGGAGCATGAGAAGGTCAA GCTGGCTGATGAGCCCACCCAGAGGAGCCAGGGAGGTGGGGATCGGAGCAGGAGGAGCCGTGCAGCCAGC TCTGCACCATCAGGCCGGAGCCAGCCAGGCAGCATAGCATCCTCAGGGTCCCAGCACACCAATGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219751 representing NM_153589
Red=Cloning site Green=Tags(s) MAAPGLRDIPLLPGSPRRLSSRTVARGSQGPKHGQQYLKVPGHRAPGQRDNSSLHPSQVSRRESSRDRSV INNYLDANEPPSSEARLSRMHFHDNQRKVDYVLAYHYRKRGAHLGHGSPGHSLAVISNGETGKERHGGGP GDVELGPLDALEEERREQRDEFEHNLMAAGLELEKDLESKSQGSVFVRIHAPWQVLAREAEFLKIKVPTK KMYEIKAGGSIAKKFSAILQTLSSPLQPRVPEHSNNRMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRI VHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPGDDMNDRKLLYQEWARYGVFYKFQPIDL IRKYFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSKEMCDHQNAFTMCPLCDKSCDYW NLSSACGTARASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERSQEHSRPEYE TKVREKLLKESGKSAVQKLEANSPEDDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRIT TAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKF VNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPK LKKLFRKLKDETEPGESDPDHSKRPEQWDLDHSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALL NNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVIINAFVIAVTSDFIPRLVYQYSYSHNG TLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWSVLSARLAFVII FQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKVKLADEPTQRSQGGGDRSRRSRAAS SAPSGRSQPGSIASSGSQHTNV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153589 |
| ORF Size | 3006 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153589.2, NP_705817.2 |
| RefSeq Size | 3949 bp |
| RefSeq ORF | 3009 bp |
| Locus ID | 243634 |
| UniProt ID | Q8CFW1 |
| Gene Summary | Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205812 | Ano2 (untagged) - Mouse anoctamin 2 (Ano2), (10ug) |
CNY 2,000.00 |
|
| MR219751 | Ano2 (Myc-DDK-tagged) - Mouse anoctamin 2 (Ano2) |
CNY 6,976.00 |
|
| MR219751L3 | Lenti ORF clone of Ano2 (Myc-DDK-tagged) - Mouse anoctamin 2 (Ano2) |
CNY 10,640.00 |
|
| MR219751L4 | Lenti ORF clone of Ano2 (mGFP-tagged) - Mouse anoctamin 2 (Ano2) |
CNY 10,640.00 |
